Detection Method of Fingerprint of Xiaoer Ganmaoshu Granules
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A technology of Xiaoer Ganmaoshu Granules and Ganmaoshu Granules, which is applied in the detection field of traditional Chinese medicine, to achieve the effect of avoiding one-sidedness, good reproducibility, and reducing the possibility
Active Publication Date: 2022-05-17
JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD +1
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[0004] At present, the fingerprint detection method of Xiaoer Ganmaoshu Granules has not been reported yet. In order to objectively, comprehensively and accurately evaluate the quality of Xiaoer Ganmaoshu Granules, control and guarantee the quality of Xiaoer Ganmaoshu Granules and ensure the clinical efficacy, it is necessary On-Development Fingerprint Research
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Embodiment 1
[0043] Embodiment 1 A detection method for the fingerprint of Xiaoer Ganmaoshu Granules, comprising the following steps:
[0044] Step 1, preparation of Xiaoer Ganmaoshu Granules test solution:
[0045] Take the 12 batches of Xiaoer Ganmaoshu granules listed in Table 1 above, accurately weigh 5g of Xiaoermaoshu granules samples, place them in a 50mL conical flask, add 30mL of methanol, ultrasonicate for 30min, filter, evaporate the filtrate in a water bath, dissolve and evaporate with methanol Dry the matter and make it to a volume of 25 mL, then take 1 mL of each sample solution, make it to a volume of 5 mL, and pass through a 0.22 μm microporous membrane to obtain the test solution.
[0046] Step 2, preparation of reference substance solution:
[0047] Accurately weigh the reference substances of puerarin, chlorogenic acid, liquiritin, arctiin, and harpagoside, place them in a volumetric flask, dilute to the mark with methanol, and shake well to make 75.02 μg / mL of puerarin...
Embodiment 2
[0054] Example 2 Legal Research on Fingerprint Detection Method
[0055] 1. Precision research
[0056] The sample number prepared by the method of Example 1 is S1 need testing solution, analyzed according to the detection method of Example 1, parallel sampling 6 times, the injection volume is 10 μ L, with chlorogenic acid, puerarin, liquiritin, arctiin , harpagoside as the reference peak, by analyzing the peak area and retention time of the common peaks of the sample HPLC fingerprints and calculating the RSD value, the results are shown in Table 3, using the "Chinese medicine chromatographic similarity evaluation software 2004A" for fingerprint comparison and data Analysis, the result similarity is 0.96, the result shows that the parallel injection precision of this equipment is good.
[0057] Table 3 Precision Study Peak Area and Retention Time
[0058]
[0059] 2. Stability research
[0060] The sample number prepared by the method of Example 1 is S1 need testing solu...
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Abstract
The invention discloses a method for detecting the fingerprint of Xiaoer Ganmaoshu Granules. The method comprises the following steps: step 1, preparation of the test solution of Xiaoer Ganmaoshu Granules; step 2, preparation of a single-standard reference substance solution; step 3, precision Draw the test solution into the liquid chromatograph, and record the chromatogram; step 4, export the fingerprint instrument of Xiaoermaoshu granules obtained in step 3, and import it into the similarity evaluation system of Chinese medicine chromatographic fingerprints, and select different batches of Xiaoermaoshu The chromatographic peaks that exist in the chromatograms of Shu granules are taken as common peaks; the control fingerprint of Xiaoermaoshu granules is generated by the average value calculation method; the relative retention time and relative peak area of each common peak are calculated. The fingerprint of Xiaoer Ganmaoshu granules provided by the present invention can comprehensively and objectively characterize the quality of Xiaoer Ganmaoshu granules. The fingerprint detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Description
technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of the fingerprint of Xiaoermaoshu granule. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic study of the chemical components of traditional Chinese medicine and is mainly used to evaluate the authenticity, excellence and stability of the quality of Chinese medicinal materials and Chinese medicinal preparations. Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation of its ...
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