Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for evaluating quality of loquat leaf medicinal materials

A technology of quality evaluation and loquat leaf, which is applied in the field of identification and quality evaluation of loquat leaf medicinal materials, achieves the effects of high precision, simple method, cost and time saving

Inactive Publication Date: 2009-01-28
PUTIAN INST OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on identifying and evaluating the quality of loquat leaves using capillary electrophoresis fingerprints. The applicant investigated and compared loquat leaves from different origins, and built a loquat leaf GAP demonstration planting base in Putian, Fujian in 2002, implementing GAP management

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating quality of loquat leaf medicinal materials
  • Method for evaluating quality of loquat leaf medicinal materials
  • Method for evaluating quality of loquat leaf medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Loquat Leaf High Performance Capillary Electrophoresis Fingerprint Method Establishment

[0031] 1 Instruments and reagents

[0032] P / ACE MDQ capillary electrophoresis system (Beckman Coulter Co., Ltd., USA), diode array detector; uncoated quartz capillary (Hebei Yongnian Optical Fiber Factory), METTLERAL204 electronic balance (Germany); KUDOS SK3310HP ultrasonic instrument (Shanghai); GZX-9070MBE constant temperature blast drying oven (Shanghai); Aikepu ultra-pure water machine; fingerprint computer-aided similarity evaluation system software (recommended software by the National Pharmacopoeia Commission). Methanol is chromatographically pure, n-butanol is analytically pure, and water is ultrapure water. The reference substance ursolic acid was purchased from China Institute for the Control of Pharmaceutical and Biological Products, batch number 110742-200415.

[0033]The loquat leaf test samples were collected from the old leaves of hanging trees in th...

Embodiment 2

[0057] The establishment of embodiment two loquat leaves HPLC fingerprint method

[0058] 1. Instruments and reagents

[0059] HP1100 high performance liquid chromatography, Eclipse XDB-C18 column (4.6mm×150mm, 5μm), diode array detector; METTLER AL204 electronic balance (Germany); KUDOS SK3310HP ultrasonic instrument (Shanghai); GZX-9070MBE constant temperature drum Air drying oven (Shanghai); fingerprint computer-aided similarity evaluation system software (compiled by Fujian Key Laboratory of Medical Testing). Methanol is chromatographically pure, glacial acetic acid is analytically pure, and water is purified water. The reference substance ursolic acid was purchased from China Institute for the Control of Pharmaceutical and Biological Products, batch number 110742-200415.

[0060] The loquat leaves tested were collected from Changtai Town Loquat Leaf GAP Medicinal Material Base (high mountain area), Xindu Town Fruit Farm (plain area), Putian Fruit Tree Research Institute...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for building a fingerprint of loquat leaf medicinal materials and a fingerprint thereof. The method comprises the following steps of: firstly collecting the loquat leaf medicinal materials for over 10 batches, preparing test sample solution and a reference product solution, implementing measurement by referring to and applying a high-efficiency liquid chromatography method and a high-efficiency capillary electrophoresis method, and finally implementing analysis by applying a computation software of the similarity between the fingerprint of traditional Chinese medicines (which is recommended by the State Pharmacopoeia Committee). The method of the invention has the advantages of simplicity, stability, high precision, good reproducibility and easy grasp, can grasp the variety and the quality condition of the loquat leaves from the integral characteristic face and the high profile of chromatogram, and can be used as one of the methods for evaluating the quality of the loquat leaves and distinguishing the loquat leaves between true and false.

Description

technical field [0001] The present invention relates to the identification and quality evaluation method of loquat leaf medicinal materials, in particular to the method for identifying and evaluating the quality of loquat leaves by using fingerprints, in particular to using high-efficiency capillary electrophoresis fingerprints or high-performance liquid chromatography fingerprints to identify the authenticity of loquat leaves and quality-constant methods. Background technique [0002] Loquat leaves are the dried leaves of Eriobotrya japonica (Thunb.) Lindl., a plant of the Rosaceae family. Hot vomiting, dysphoria and thirst, etc. (Edited by the National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China. The first part. Beijing: Chemical Industry Press, 2005: 142). In recent years, a large number of studies have found (Lin Yulin, Lin Wenjin, Lin Liqiang. Research status and development prospects of loquat leaves, Chinese medicinal materials. 2006, 29(...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00G01N1/28G01N30/06
Inventor 林玉霖徐榕青林文津林力强
Owner PUTIAN INST OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com