A kind of detection method of anti-lung cancer sea Mu recipe fingerprint

A technology of fingerprint spectrum and detection method, applied in the field of detection of traditional Chinese medicine compounds, can solve the problems of no anti-lung cancer Haimu prescription fingerprint spectrum, individual components cannot fully reflect the comprehensive information of drugs, complex components of Haimu prescription, etc., and achieve repeatability. and stability, the method is simple, the effect of accurate detection and evaluation

Active Publication Date: 2022-07-05
QINGDAO MARINE BIOPHARMACEUTICAL RES INST +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] However, the compound ingredients of Haimufang are complicated, and the qualitative and quantitative analysis of individual components is difficult to fully reflect the comprehensive information of the drug, and there is no related research on the fingerprint of Haimufang for anti-lung cancer.

Method used

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  • A kind of detection method of anti-lung cancer sea Mu recipe fingerprint
  • A kind of detection method of anti-lung cancer sea Mu recipe fingerprint
  • A kind of detection method of anti-lung cancer sea Mu recipe fingerprint

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Experimental program
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Embodiment 1

[0046] Step 1. Preparation of reference peak solution: take a certain amount of solanine, solanine, solanine, and solanine, and dissolve in methanol to prepare a mixed reference solution containing 0.5 mg per milliliter.

[0047] Step 2. Take 10 batches of Haimu Fang for testing (batch number: NO.1: 20180701, NO.2: 20180702, NO.3: 20180703, NO.4: 20180704, NO.5: 20181001, NO. 6: 20181002, NO.7: 20190101, NO.8: 201904001, NO.9: 201904002, NO.10: 201908001), take about 50 mg, accurately weigh, add 2 mL of 5% methanol to dissolve; Cleanert S C18 solid phase extraction Column treatment, the treatment process is: methanol activation, water equilibration, sample loading, eluting with 2 column volumes of water and 15% methanol aqueous solution, 3 column volumes of 0.5% formic acid methanol, collecting the eluent, N 2 Blow dry, add 70 to residue μ Redissolve in L methanol, centrifuge, and take the supernatant to obtain 10 batches of the test solution.

[0048] Step 3. Precisely draw...

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Abstract

The invention discloses a method for detecting the fingerprint of anti-lung cancer sea mulberry recipe, comprising the following steps: step 1: preparation of a reference peak solution; step 2: preparation of a test solution of anti-lung cancer ha mu recipe; step 3: separately precise Inject the quantitative reference peak solution and the test solution into the liquid chromatograph, and record the chromatogram; Step 4: Export the Haimu Fang fingerprint instrument obtained in step 3, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, select different The chromatographic peaks that exist in the chromatograms of the batches of Haimufang tested are regarded as common peaks, and the average value calculation method is used to generate the control fingerprint of Haimufang, and the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint spectrum of the Haimu prescription provided by the invention can comprehensively and objectively characterize the quality of the Haimu prescription, and is beneficial to comprehensively monitor the quality of the medicine. The fingerprint detection method provided by the invention has the advantages of simple method, stability, high precision, good reproducibility and the like.

Description

technical field [0001] The invention relates to a method for detecting a traditional Chinese medicine compound, in particular to a method for detecting the fingerprint of anti-lung cancer Haimu prescription and its standard fingerprint. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the chromatographic or spectral spectrum of a certain type or several types of components that are common in a certain Chinese medicinal material or proprietary Chinese medicine. At the present stage, when most of the active ingredients of traditional Chinese medicine are not clear, the fingerprint of traditional Chinese medicine is of great significance for effectively controlling and evaluating the quality of traditional Chinese medicinal materials or Chinese patent medicines. [0003] Anti-lung cancer Haimu prescription is a clinical experience prescription of a famous old Chinese medicine. The whole prescription is composed of seaweed and oyster, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8679G01N2030/324Y02A50/30
Inventor 刘红兵管华诗胡淑曼马晓青魏宝红
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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