Detection Method of Fingerprint of Lingguizhugan Decoction
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A fingerprint and detection method technology, which is applied in the detection field of traditional Chinese medicine prescriptions, can solve the problems of less research on detection methods, and achieve the effect of good reproducibility, high precision and good stability
Active Publication Date: 2021-04-06
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +2
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[0004] At present, there are few studies on the fingerprint detection methods of Lingguizhugan Decoction
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Embodiment 1
[0043] 1, a kind of detection method of Linggui Zhugan Decoction fingerprint, is characterized in that, comprises the following steps:
[0044] Step 1, the preparation of Linggui Zhugan Decoction test solution:
[0045] Precisely weigh 10 batches of Poria cocos, Guizhi, Atractylodes macrocephala and licorice, including 40g of Poria cocos, 30g of cinnamon twig, 20g of Atractylodes macrocephala and 20g of licorice. Add 1L of water for ultrasonic extraction, collect the medicinal solution by filtration, evaporate the medicinal solution to dryness, add 4 times the amount of 95% ethanol and heat under reflux for extraction for 3 hours, filter the supernatant, evaporate to dryness in a water bath, add 20mL methanol solution to dissolve, pass through 0.45μm Pore filter membrane, get 10 batches of need testing solution;
[0046] Step 2, preparation of reference substance solution:
[0047] , the preparation method of the reference substance solution in step 2 is as follows: take acc...
Embodiment 2
[0064] Embodiment 2 methodological investigation
[0065] 1. Precision experiment
[0066] Precisely draw the reference substance solution under embodiment 1, and continuously inject samples 6 times by the chromatographic conditions under embodiment 1, the RSD of its main common peak retention time is all less than 0.95%, and the relative peak area RSD is all less than 2.44%, showing the precision of the instrument The degree is good. The specific experimental results are shown in Table 6 and Table 7.
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Abstract
The invention discloses a method for detecting fingerprints of Linggui Zhugan Decoction. The establishment of fingerprints includes the following steps: step 1, preparation of Linggui Zhugan Decoction test solution; step 2, preparation of reference solution; step 3, precise absorption respectively The test solution and the reference solution are injected into the liquid chromatograph, and the chromatogram is recorded; step 4, the Linggui Zhugan Decoction fingerprint instrument obtained in step 3 is exported, and imported into the Chinese medicine chromatographic fingerprint similarity evaluation system, and different batches are selected. The chromatographic peaks that all exist in the chromatograms of Linggui Zhugan Decoction of the second time are used as common peaks, and the control fingerprint of Linggui Zhugan Decoction is generated by the average value calculation method; the relative retention time and relative peak area of each common peak are calculated. The detection method of the fingerprint of Linggui Zhugan Decoction provided by the present invention has the advantages of good stability, high precision, good reproducibility, etc., can accurately analyze the active ingredients in Linggui Zhugan Decoction, and can comprehensively and objectively evaluate Linggui Zhugan Decoction. quality is of great significance.
Description
technical field [0001] The invention relates to a detection method of a traditional Chinese medicine prescription, in particular to a detection method of a fingerprint of Linggui Zhugan Decoction. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine, and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and traditional Chinese medicine preparations. Traditional Chinese medicine and its preparations are multi-component complex sys...
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