Quantitative detection method for multiple components of Qiju Dihuang Oral Liquid

A technique for rehmannia oral liquid and a detection method, which is applied in the detection field of traditional Chinese medicine, can solve problems such as the inability to comprehensively and objectively evaluate the quality of Qiju Dihuang oral liquid, and achieve the effects of simple method, avoiding one-sidedness, and good reproducibility

Active Publication Date: 2019-04-05
JIANGSU HAISHENG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current quality identification methods for Qiju Dihuang Oral Liquid only measure the content of a single or several active ingredients, and cannot comprehensively and objectively evaluate the quality of Qiju Dihuang Oral Liquid

Method used

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  • Quantitative detection method for multiple components of Qiju Dihuang Oral Liquid
  • Quantitative detection method for multiple components of Qiju Dihuang Oral Liquid
  • Quantitative detection method for multiple components of Qiju Dihuang Oral Liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 A kind of detection method of Qiju Dihuang oral liquid multi-component quantification comprises the following steps:

[0047] Step 1, the preparation of Qiju Dihuang Oral Liquid need testing solution:

[0048] Take 10 mL of Qiju Dihuang oral liquid from the above three batches in Table 1, measure them accurately, place in an evaporating dish, evaporate to dryness in a water bath, add 5 ml of methanol for ultrasonic treatment (power 150W, frequency 20kHz) for 30 minutes, let cool, filter to obtain clear liquid, and set Make up to 10mL, pass through a 0.45μm microporous membrane to obtain the test solution.

[0049] Step 2, preparation of acid mixed reference substance solution:

[0050] Take an appropriate amount of chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, salvianolic acid B, and paeonol, weigh them accurately, and add methanol to make each 1 mL of green A mixed reference solution of 90u...

Embodiment 2

[0059] Embodiment 2 methodological investigation

[0060] 1. Precision research

[0061] The sample batch number prepared by the method of Example 1 is 140401 need testing solution, analyzed according to the detection method of Example 1, parallel injection 6 times, the injection volume is 10 μ L, with 5-HMF, morroniside, chlorogenic acid, RSD ( %) values ​​were 1.32, 2.58, 3.45, 2.01, 1.94, 1.53, 1.49, 2.05, 2.53, 2.41, 0.98, 2.40, 2.58. The results showed that the parallel injection precision of the equipment was good.

[0062] 2. Stability research

[0063] The batch number of the sample prepared according to the method of Example 1 is 140401 test solution, analyzed according to the detection method of Example 1, using 0, 2, 6, 12, 18, and 24 hours for sample injection analysis, and the injection volume is 10 μL , with 5-HMF, morroniside, chlorogenic acid, cryptochlorogenic acid, loganin, paeoniflorin, verbascoside, luteolin, isochlorogenic acid B, isochlorogenic acid A,...

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Abstract

The invention discloses a multicomponent quantitative detection method of a lycium-rehmannia oral liquid. The establishment of the multicomponent quantitative method of the lycium-rehmannia oral liquid comprises the following steps: Step 1, preparing a test solution of the lycium-rehmannia oral liquid; Step 2, preparing a mixed reference solution; Step 3, respectively and precisely absorbing the test solution and the mixed reference solution and injecting into a liquid chromatogram, and recording a chromatogram; and Step 4, calculating contents of components in a sample through the chromatogram of the mixed reference substance and the lycium-rehmannia oral liquid according to retention time and peak area. Through the multicomponent quantitative analysis method of the lycium-rehmannia oralliquid, quality of the lycium-rehmannia oral liquid can be represented comprehensively and objectively. The multicomponent quantitative detection method provided by the invention has advantages of simple and stable method, high precision, good repeatability and the like.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a quantitative detection method of multi-component Qiju Dihuang Oral Liquid. Background technique [0002] Qiju Dihuang Pill corresponds to the treatment of yin deficiency and hyperactivity of fire, plus clearing the liver and improving eyesight. Hyperactivity of fire due to yin deficiency refers to the imbalance of yin and yang, yin deficiency leads to hyperactivity of yang and heat (formation) fire. In addition to the phenomenon of internal heat caused by yin deficiency when traveling, there are still flushing, red eyes, dry throat, sore throat, bleeding, upset, less fur, tongue texture Red thin, rapid pulse and other evidence. Excessive fire due to yin deficiency refers to the deficiency of yin in the viscera, loss of nourishment, and endogenous deficiency and heat. This can occur in the heart, liver, lung, spleen, and kidney. Kidney yin deficiency is mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 郑艳萍蔡宝昌王欣玲金俊杰秦昆明杨冰孙戡平彭思颖
Owner JIANGSU HAISHENG PHARMA
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