Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas

A technology of Ganoderma lucidum spore oil and Ganoderma lucidum spores, which is applied in the field of standard fingerprints, can solve the problems of few research reports on the quality of Ganoderma lucidum spore oil, and achieves the effects of being easy to master, having good reproducibility and reducing possibility

Active Publication Date: 2008-01-16
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many reports on the quality research of Ganoderma lucidum spores and their fat-soluble extract Ganoderma lucidum spore oil, especially the variety and quality of Ganoderma lucidum spores and their fat-soluble extract Ganoderma spore oil can be grasped from the overall characteristics of the chromatography.

Method used

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  • Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas
  • Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas
  • Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the establishment method of Ganoderma lucidum spore HPLC standard fingerprint

[0026] 1. Instruments and reagents

[0027] HP 1100 High Performance Liquid Chromatograph; Evaporative Light Scattering Detector; Aotai Chromatography Star Workstation. Triolein reference substance, purity greater than 99%; Acetonitrile, chromatographically pure; Isopropanol, chromatographically pure; Ganoderma lucidum spores, batch numbers: 01, 02, 03, 04, produced in Guangdong; 07, produced in Shandong; Ganoderma lucidum spores, batch numbers: 08, 09, 10, produced in Anhui; Ganoderma lucidum spore oil, provided by Guangzhou Hanfang Modern Chinese Medicine Research and Development Co., Ltd.

[0028] 2. Chromatographic conditions

[0029] Column: kromasil C 18 Column (4.6×250mm, 5μm), American Alltech Company; mobile phase: acetonitrile-isopropanol (53:47); detection by evaporative light scattering detector; flow rate: 0.8ml / min; column temperature: 30°C; theoretical plate ...

Embodiment 2

[0052] Embodiment 2, the establishment method of Ganoderma lucidum spore oil HPLC standard fingerprint

[0053] The method is the same as the establishment method of the Ganoderma lucidum spore HPLC standard fingerprint, the difference is:

[0054] Preparation of Ganoderma lucidum spore oil test solution: Take 120 mg of Ganoderma lucidum spore oil, accurately weigh, add mobile phase: acetonitrile-isopropanol (53:47) to dissolve and set the volume to 50ml, shake well, filter, and take the subsequent filtrate as the supply Test solution

[0055] Take 10 μl each of the reference substance solution and the Ganoderma lucidum spore oil test solution for sample injection, measure according to high performance liquid chromatography, and record the chromatogram for 60 minutes, see accompanying drawing 3. Calculate the relative retention time of other chromatographic peaks and calculate the relative peak area with the relative retention time of the chromatographic peak (S peak) of trio...

Embodiment 3

[0065] Get Ganoderma lucidum spores from a certain production area in Anhui, according to the conditions and methods in the establishment method of the HPLC standard fingerprint of Ganoderma lucidum spores in Example 1, get 0.4g of Ganoderma lucidum spores, add mobile phase: acetonitrile-isopropanol (53:47) 50ml, precision Weigh, soak for 2 hours, sonicate (power 300W, frequency 50kHz) for 50 minutes, take it out, let it cool, weigh it, add mobile phase to make up the lost weight, shake well, filter, and get the continued filtrate as the test solution Get respectively 10 μ l sample injections of reference substance solution and above-mentioned need testing solution, measure according to high performance liquid chromatography, record the chromatogram of 60 minutes, get fingerprint chromatogram, see Fig. 4. Calculate the relative retention time of other chromatographic peaks and calculate the relative peak area with the relative retention time of the chromatographic peak (S peak)...

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Abstract

The invention is related to method for controlling quality of traditional Chinese medicinal materials and productions. Comparing fingerprints of high performance liquid chromatography HPLC for ten batches of ganoderma lucidum spore, oil of ganoderma lucidum spore determines mutual characters so as to obtain standard fingerprints. There are 15 mutual peaks in fingerprints. There are 4 peaks with its area exceeding 5% of total peak area. Features of the invention are: simple, stable, high accuracy, good reproducibility, easy of mastering. The invention discloses total new method for controlling quality of ganoderma lucidum spore, and oil of ganoderma lucidum spore, and distinguishing true or false products.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine and its products, in particular to a method for constructing a high-performance liquid chromatography (HPLC) fingerprint of ganoderma lucidum spores or ganoderma spore oil and the obtained standard fingerprints. Background technique [0002] Ganoderma lucidum is the general term for red lucidum Glucidum.karst and purple lucidum G.japonicum Lloyd of the basidiomycete family Polyporaceae (Polyporaceae) Ganoderma (Ganoderma). Ganoderma lucidium spores are extremely small spores ejected from the cap during the mature growth stage of Ganoderma lucidum. They are the germ cells of Ganoderma lucidum and contain all the genetically active substances of Ganoderma lucidum. Their medicinal value is also increasingly valued. The pharmacological effects of Ganoderma lucidum spores mainly include anti-tumor, immune regulation, regulation of blood lipids, and regulation and improvement o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 李吉来宁德山刘菊妍陈路林吴德玄黄海
Owner GUANGZHOU HANFANG PHARMA
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