Detection method of fingerprint spectrum of double-flower sangju drink
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A technology of fingerprints and detection methods, which is applied in the detection field of fingerprints of Shuanghua Sangju Decoction, can solve the problems of few quality detection methods of Shuanghua Sangju Decoction, etc., and achieve the possibility of reducing the possibility of manual processing, good reproducibility, avoid one-sided effects
Pending Publication Date: 2021-06-01
南京南医堂健康产业发展有限公司
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[0003] At present, there are few quality testing methods for Shuanghua Sangju Decoction
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Embodiment 1
[0043] Embodiment 1 A kind of detection method of fingerprint of Shuanghua Sangju Drink comprises the following steps:
[0044] Step 1, the preparation of Shuanghua Sangju drink test solution:
[0045] Take 8 batches of Shuanghua Sangju Decoction samples (made from 30 parts of honeysuckle, 35 parts of mulberry leaves, 10 parts of chrysanthemum, 10 parts of mint, and 15 parts of cassia seed) 5g into a 100mL round bottom bottle, add 50mL of methanol solution, and reflux extraction 2h, filter, put the filtrate in a 100mL volumetric flask, add methanol to constant volume, and pass through a 0.45μm microporous membrane to obtain the test solution;
[0046] Step 2, preparation of mixed reference solution:
[0047] Take accurately weighed reference substances of chlorogenic acid, rutin, luteolin, cryptochlorogenic acid, and arurinine cassiatin, place them in a volumetric flask, set the volume to the mark with methanol, and shake well to prepare 0.0674mg / mL Chlorogenic acid, 0.0902 mg...
Embodiment 2
[0054] Legal research of embodiment 2 fingerprint spectrum detection method:
[0055] 1. Precision research
[0056] Get Shuanghua Sangju drink sample (30 parts of honeysuckle, 35 parts of mulberry leaves, 10 parts of chrysanthemum, 10 parts of peppermint, 15 parts of cassia seed are made), the sample number prepared by the method of embodiment 1 is S1 need testing solution, according to implementation Analysis of the detection method in Example 1, 6 parallel injections, the injection volume is 10 μL, with chlorogenic acid, rutin, luteolin, cryptochlorogenic acid, and orange cassiatin as reference peaks, by analyzing the HPLC fingerprint of the sample The peak area and retention time of the common peaks were calculated and the RSD value was calculated. The results are shown in Table 2. The results show that the parallel injection precision of the equipment is good.
[0057] Table 2 Precision Study Peak Area and Retention Time
[0058]
[0059] 2. Stability research
[00...
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Abstract
The invention discloses a detection method of a fingerprint spectrum of a double-flower sangju drink. The detection method comprises the following steps: step 1, preparing a test solution of the double-flower sangju drink; step 2 preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording a chromatogram map; step 4, exporting the fingerprint spectrum instrument of the double-flower sangju drink in the step 3, importing the fingerprint spectrum instrument into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of double-flower sangju drink as common peaks; generating a contrast fingerprint spectrum of the double-flower sangju drink by using an average value calculation method; and step 5, comparing the fingerprint spectrum of the double-flower sangju drink with the spectrum of the mixed standard substance, and identifying main components. The fingerprint spectrum of the double-flower sangju drink can comprehensively and objectively characterize the quality of the double-flower sangju drink. The fingerprint detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Description
technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of the fingerprint of Shuanghua Sangju Decoction. Background technique [0002] Shuanghua Sangju Decoction is a kind of recuperating tea drink that contains the theory of traditional Chinese medicine developed with the strong support of Nanjing Nanyitang Health Industry Development Co., Ltd., based on decades of clinical experience summed up by Professor Xie Yingbiao, a famous Chinese medicine doctor in Nanjing. This product is mainly composed of honeysuckle, mulberry leaves, chrysanthemum, mint, cassia seeds and other ingredients. It has the effects of removing acne, smoothing skin, clearing heat and dampness, reducing inflammation and reducing fire, and dispersing wind-heat. It can effectively regulate human metabolic disorders and endocrine disorders, and can remove acne. Acne, acne, pimples, rashes, etc. It is often used clinically to r...
Claims
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