SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and application of primer group in germplasm identification

A technology of transcriptome sequencing and velvety wax, applied in the biological field, can solve the problems such as the inability to meet the research of ash and the small quantity.

Inactive Publication Date: 2014-09-17
CHINA AGRI UNIV +1
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  • Application Information

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Problems solved by technology

Compared with woody plants such as poplar and tea tree, white wax has only European white wax SSR markers (only 11 pairs), and Wang Jianbing et al. have developed 3 pairs of polymorphic white wax SSR markers th

Method used

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  • SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and application of primer group in germplasm identification
  • SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and application of primer group in germplasm identification
  • SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and application of primer group in germplasm identification

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Embodiment 1

[0069] 1. Extraction of white wax genomic DNA:

[0070] (1) Material

[0071] Twelve representative species were selected from the core germplasm material of Baiwa, namely Fraxinus sogdiana, Fraxinus chinensis Roxb, Fraxinus velutina Torr Hongyebaila, Fraxinus hupehensis , Fraxinus velutina Torr Lula No.2, Fraxinus velutina Torr Lula No.3, Fraxinus pennsylvanica Lula No.6, Fraxinus mandshurica Rupr , Fraxinus rhynchophylla, Ash (Fraxinus americana Linn), European white wax (Fraxinus Excelsior), golden leaf white wax (Fraxinus Linn).

[0072] In May every year, fresh leaves just grown in spring were taken, sealed in fresh-keeping bags, put in an ice box, and brought back to Shandong Academy of Forestry Sciences. After being frozen in liquid nitrogen, they were stored in a -70°C refrigerator for later use.

[0073] (2) Using the CTAB method to extract the genomic DNA of the leaves of the white wax

[0074] 1) Preheat the prepared CTAB extraction buffer solution in a water bat...

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Abstract

The invention discloses an SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and an application of the primer group in germplasm identification, belonging to the technical field of molecular biology. The SSR primer group comprises 42 pairs of primers, wherein the nucleotide sequences of the first pair to the forty-second pair of primers are shown in SEQ ID No.1 to SEQ ID No.84. Experiments verify that the SSR primer group disclosed by the invention has the advantages of rich polymorphism, good repeatability, clear electrophoretic band and the like. The SSR primer group can be effectively used for researches on germplasm identification, DNA fingerprint establishment and the like. A germplasm identification method is sensitive and reliable, can be used for rapidly and accurately realizing and finishing distinguishment and identification of Fraxinus chinensis germplasm, and has important significances in identification and intellectual property protection of Fraxinus chinensis germplasm resources and genetic breeding of genetic breeding molecules.

Description

technical field [0001] The invention relates to a set of SSR primers developed based on the sequence information of the transcriptome of A. tomentosa and its application in germplasm identification, belonging to the field of biotechnology. Background technique [0002] Fraxinus Velutina Torr. is a deciduous tree of the genus Fraxinus Linn. in the family Leaceae. It is native to the United States and was introduced to Jinan, Shandong Province in 1911 (Meng Zhaohe et al., 2001). It is fast-growing, beautiful, and stress-resistant. It has become the main landscaping tree species in the coastal cities of North China and East China and excellent saline-alkali land afforestation tree species (Shi Mingzhi, 1996; Wang Qin et al., 2000; Ni Guoxiang et al., 1995; Wang Youping, 2007), and it is also the only large tree species in saline-alkali land. Due to the lack of a large number of polymorphic molecular markers, the research on its genetics and genomics lags behind the tree species...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 燕丽萍刘翠兰夏阳李丽孙超
Owner CHINA AGRI UNIV
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