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SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group

A core primer and genome-wide technology, applied in the field of molecular biology, can solve the problem of the small number of SSR markers, and achieve the effect of rich polymorphism, uniform distribution, and easy identification

Active Publication Date: 2014-08-13
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with rice, wheat and other crops, the number of existing millet SSR markers is small, which cannot meet the needs of millet research. The development of a large number of SSR markers covering the whole genome is still one of the important tasks of millet research.

Method used

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  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group
  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group
  • SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and application of SSR core primer group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Application of millet genome SSR markers in variety identification

[0023] 1. Extraction of millet genomic DNA

[0024] (1) Material

[0025] 41 representative local varieties and bred varieties were selected from the core germplasm materials of millet, involving a total of 6 ecological regions in the Northeast Plain, North China Plain, south of the Huaihe River, Loess Plateau, Inner Mongolia Plateau and Northwest Inland (see Table 1). , for the evaluation of millet primers and the research on variety identification.

[0026] Table 1 is used for the material information of millet variety identification in the present invention

[0027]

[0028]

[0029]

[0030] (2) Genomic DNA extraction by CTAB method

[0031] The modified CTAB method was used to extract millet DNA, and the specific steps were as follows:

[0032] 1) Germinate and cultivate the tested millet materials, and extract DNA at the stage of two leaves and one heart.

[0033] 2) Cut ...

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Abstract

The invention discloses an SSR (Simple Sequence Repeat) core primer group developed based on whole genome sequence of foxtail millet and an application of the SSR core primer group, and belongs to the technical field of molecular biology. The core primer group comprises 30 pairs of primers, wherein nucleotide sequences are represented by SEQ ID NO. 1-60. The primer has advantages of clear electrophoretic band and rich polymorphism, and is uniformly distributed and stable in amplification. The invention also provides the application of the SSR core primer group in identifying the genetic diversity and variety of the foxtail millet. The primer group can be used for precisely and quickly identifying the variety of the foxtail millet and precisely reflecting a genetic relationship among the varieties of the foxtail millet.

Description

technical field [0001] The invention relates to millet SSR markers, in particular to an SSR core primer set developed based on the whole genome sequence of millet and its application, belonging to the technical field of molecular biology. Background technique [0002] Millet (Setaria Italica Beauv.) belongs to Gramineae (Gramineae) Setaria (L.) Beauv.], it is the world's oldest crop originated from China and the nourishing crop of the Chinese nation. The problem played a very important role, and it was also the strategic grain reserve at that time. Millet is rich in nutritional value, rich in vitamins A and E. The methionine in the essential amino acids for human body is 118 times that of Bayi powder and 2125 times that of rice. It has good health care effects and can be developed and processed into a series of convenient foods. Cereal grain protein also has anti-atherosclerotic effect. [0003] Millet is a C4 plant with the characteristics of drought tolerance, salt toler...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68C12N15/10
Inventor 李汝玉张晗王雪梅王东建姚凤霞许金芳孙加梅郑永胜段丽丽李华
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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