80 results about "Fraxinus chinensis" patented technology

The method provides an original soil afforesting method of moderate and severe saline and alkaline land. After tamarix chinensis seedlings in a greenhouse nursery garden are exercised and restrained in growth for root development, the seedlings are transplanted in the moderate and severe saline and alkaline land to perform original soil cultivation. Simultaneously, salix psammophila, bamboo willow and fraxinus chinensis roxb are planted between tamarix chinensis planting belts. According to the original soil afforesting method of the moderate and severe saline and alkaline land, the seedlings are selected to perform interplanting, original soil seedling planting is adopted to afforest the moderate and severe saline and alkaline land, and the problem that methods of traditional soil dressing planting, soil improvement, desalinization through irrigation and the like are high in cost and easy to suffer saline erosion is avoided. The original soil afforesting method of the moderate and severe saline and alkaline land is low in cost, becomes effective rapidly, can form novel ecological environment at the moderate and severe saline and alkaline land rapidly, achieves ecological balance, and avoids saline erosion. Four kinds of seedlings are interplanted simultaneously, and the tree species can supplement each other to achieve ecological balance so as to enable the seedlings to survive and grow easily and form a virtuous cycle.

The invention discloses a method for identifying fraxinus chinensis species by virtue of an SSR (Simple Sequence Repeat) fingerprint spectrum. The method comprises the following steps: (1) extracting total DNA of fraxinus chinensis; (2) performing PCR (Polymerase Chain Reaction) amplification on SSR sequences; (3) electrophoresing PCR amplification products, dyeing and identifying, wherein fraxinus chinensis DNA can be subjected to PCR amplification by virtue of four pairs of SSR primer combinations disclosed in the step (2) to obtain a result that the difference of the amplification products passing through polyacrylamide gel electrophoresis detection strips is remarkable, and then the SSR fingerprint spectrum of the fraxinus chinensis species is built. According to the method, fraxinus chinensis species resources are accurately identified in molecular level, and the problem of difficult identification of the fraxinus chinensis species is solved; moreover, the method is short in detection time, good in repeatability and high in accuracy, and has specificity in collected 12 species.

The invention relates to a grafting method of sweet osmanthus. The grafting method of the sweet osmanthus includes following steps: (1) selecting rootstocks: selecting glossy privet and fraxinus chinensis in the south, and selecting Chionanthus retusus in the north; (2) selecting scions: selecting excellent single plants which grow strongly, do not suffer from plant diseases or insect pests, are pure in breed and flower exuberantly from a scion picking seed tree; (3) gathering the scions: gathering scions used for spring grafting before spring budding, and using the other scions for autumn plate budding and stem grafting once the autumn scions are picked; (4) processing the scions: cutting each scion for the stem grafting into a shoot section with 4-6 buds in the grafting, sealing the shoot sections with paraffin, and using a water bath method to melt the paraffin; (5) using a notch grafting method. Compared with other propagation methods, the grafting method of the sweet osmanthus has the advantages of being simple to operate and large in propagation amount, obtaining grafting trees rapid in growth and the like.

The present invention discloses a composition with functions of preventing wrinkles and removing dark eye circles and eyelid pouches, and a preparation method and an application thereof. The composition is composed of a Chinese ash bark extract, caffeine silanol C, an aesculus hippocastanum extract, superoxide dismutase, a humectant, a chelating agent, a preservative and water. The composition caninhibit melanin production from multiple angles, reduces pigmentation, enables skin color to be even, reduces eye edema, diminishes the wrinkles, fine lines and dark eye circles, conducts deep moisturizing, improves skin moisturizing degrees, and thus enables eye skin to be full of vitality.

The invention discloses a salt-tolerant rhizosphere growth-promoting enterobacter cloacae and an application thereof. The strain is classified and named as enterobacter cloacae TIA062, which is preserved in China General Microbiological Culture Collection Center on January 7, 2016 with a preservation number of CGMCC No.11974. The salt-tolerant rhizosphere growth-promoting enterobacter cloacae disclosed by the invention can improve the saline-alkaline tolerance of fraxinus chinensis. Compared with the fraxinus chinensis which is not inoculated with inoculant, the fraxinus chinensis of which rhizosphere is inoculated with the salt-tolerant rhizosphere growth-promoting enterobacter cloacae disclosed by the invention has the advantages that rhizosphere water content of the fraxinus chinensis can be improved and the growth of the fraxinus chinensis can be promoted after salt stress for a certain time; therefore, the enterobacter cloacae can be applied to a salt environment as an inoculant, so that a high-quality strain source is provided for improving the saline-alkaline tolerance of the fraxinus chinensis.

The invention discloses a method for the tissue culture and the rapid breeding of Fraxinus chinensis. According to the method, axillary buds or stem tips of Fraxinus chinensis are propagated into nursery seedlings through several steps: material disinfection, startup culture, proliferation and differentiation culture, rooting culture, transplantation, strong seedling culture and the like, and the survival rate of the transplantation of test-tube seedlings is over 90%. The method has the advantage of high breeding speed, so that breeding can be carried out annually, the breeding process of the Fraxinus chinensis can be significantly accelerated, and the problem of shortage of green plant seedling sources for large area afforestation on saline-alkali lands in China is effectively solved. The method can also greatly reduce the cost for afforestation and save public resources.

The invention discloses a planting method for increasing the bare-rooted seedling afforestation survival rate with water-saving irrigation. The planting method comprises the following steps: I, selecting tree species: selecting any one of the trees species pinus thunbergii, platycladus orientalis, elm, robinia pseudoacacia, cotinus coggygria and fraxinus chinensis; II, digging a planting hole: confirming the shape and the specification of the planting hole according to the type of the afforestation land and the specification of a seedling, wherein the planting hole generally takes the shape of a circular truncated cone in a plain area, the specification of the planting hole can be slightly large in a non-salt and alkali area, the diameter of the upper part of the planting hole is 60-80 cm, the diameter of the lower part of the planting hole is 40-60 cm, and the depth of the planting hole is 60-80 cm, and in a plain salt and alkali area, the diameter of the upper part of the planting hole is 50-60 cm, the diameter of the lower part of the planting hole is 40-50 cm, and the depth of the planting hole is 50-60 cm. By adoption of the planting method, limited moisture can be concentrated around the root system of the seedling and is not easy to evaporate or diffuse, water supply to the root system of the seedling in the key survival period can be assisted, sucking of weeds to moisture and nutrient in soil can be reduced, an ideal small environment is provided for survival and growth of seedling root systems, the afforestation survival rate can be ensured, and the problems that the afforestation irrigation cost is high and the afforestation survival rate is low in a water-deficient area are solved.

The invention provides a composition for removing dark circles and under-eye puffiness as well as a preparation method and application thereof. Based on the total mass of the composition for removingdark circles and under-eye puffiness, the composition for removing dark circles and under-eye puffiness is prepared from 0.01%-3% of fraxinus chinensis bark extract, 0.01%-1.5% of an asparagopsis armata extract, 0.01%-5% of glucosyl hesperidin, 0.001%-0.05% of acetyl tetrapeptide-5, and 0.1-10% of a coffee extract. According to the composition for removing dark circles and under-eye puffiness, allthe components are mixed for use, the purpose of synergistic interaction can be achieved, and therefore the problems of the dark circles and the under-eye puffiness are effectively solved.

The invention provides a composition for resisting wrinkles, removing dark circles and removing eye bags and application thereof. The composition comprises propyl gallate, epigallocatechin gallate glucoside, diglucosyl gallic acid, fraxinus chinensis bark extract, glucosyl hesperidin and palmitoyl tripeptide-5. The composition is applied to a nursing product and has the effects of replenishing water, reducing fine wrinkles, reducing dark circles and astringing eye bags; effective components can penetrate into eye skin bottoms to care eye skin.

The invention discloses a shrub type tree form pruning method of a Chinese ash. The shrub type tree form pruning method of the Chinese ash comprises the following steps: (1) confirming a base trunk of the Chinese ash used for growing branches, (2) pruning an upright main root of the Chinese ash and reserving branch roots of the Chinese ash, (3) selecting sturdy branches as reserved first-level branches from the branches grown from the base trunk in different directions through a direction complementary principle after the branches grow a period of time, pruning the other grown branches from the base trunk growing parts, and shortening the selected first-level branches after the first-level branches grow 1-2 years, (4) selecting reserved second-level branches according to the selection principle of the first-level branches, and (5) processing routine pruning and management to each level of follow-up growing branches according to the shrub type tree form. By means of the shrub type tree form pruning method of the Chinese ash, reuse of a Chinese ash with a damaged trunk is realized, waste of plantlet resources in avoided, economic benefit is notable, adaptive capacity of Chinese ashes to environment of coastal saline areas is improved through transform of the tree form, large shrub species needed in green construction are enriched, and Chinese ashes have notable landscape value and ecological benefits after being pruned.

The invention provides facial cream. According to the facial cream, 0.1%-1.0% of tocopheryl acetate, 0.5%-5.5% of simmondsia californica seed oil, 0.1%-5.0% of acmella oleracea extract, 2.0%-5.0% of butanediol, 0.1%-4.0% of nicotinamide, 0.1%-5.0% of fraxinus excelsior bark extract, 0.01%-0.1% of potassium citrate, 0.1%-5.0% of oat kernel extract, 0.01%-5.0% of roe extract and 0.01%-5.0% of hydrolyzed soybean protein are added according to the component ratios; and the plant extracts (including the fraxinus excelsior bark extract, the acmella oleracea extract and the oat kernel extract) are used as the components and have the effects of maintaining moisture and moisturizing and regaining the moisture of skin.

The invention discloses fraxinus mandshurica extract for treating rheumatoid arthritis and belongs to the technical field of traditional Chinese medicines. The fraxinus mandshurica extract comprises the following compounds in percentage by weight: 50%-95% of fraxinol glucoside, 2%-15% of fraxin, 0.1%-1% of isoscopolin and 0.1%-1% of isofraxidin-beta-D-glucoside. The fraxinus mandshurica extract has the beneficial effects of being low in toxicity, safe and reliable in comparison with chemical medicines such as hormones and the like while being applied to the medicine for treating rheumatoid arthritis; moreover, the fraxinus mandshurica leaf resources are abundant, the waste material is changed into things of value and the extraction process is simple; the fraxinus mandshurica extract and pharmaceutically acceptable excipients or auxiliaries materials can be used for preparing a medical composition; and the medical composition can be prepared into various dosage forms such as gastrointestinal tract dosage forms consisting of tablets, pills, capsules, particles, tinctures and oral liquid as well as gastrointestinal tract external dosage forms consisting of injections, external-use preparations and the like by adopting the conventional method of the galenic pharmacy.

The invention provides application of fraxin in preparation of drugs for preventing or treating an acute respiratory distress syndrome (ARDS). The fraxin is a coumarins compound which is extracted from dry barks of oleaceae fraxinus chinensis, Fraxinus szaboana Lingelsh or Fraxinus stylosa Lingelsh. Existing researches show that the fraxin has effects of resisting inflammation, reducing blood uricacid, resisting bacteria and inducing diuresis. On the basis of constructing an anima damage model of acute respiratory distress syndrome, by HE, Evan blue staining, fluorescence probes, immunofluorescent labeling, western blotting and the like, it verifies that the fraxin can reduce generation and releasing of reactive oxygen (ROS), inhibit generation and releasing of inflammatory factors and inhibit activation of signal paths MAPKs, therefore, degradation of a blood vessel endothelial polysaccharide coating is relieved, and vascular permeability is relieved to prevent or treat ARDS.

The invention discloses an SSR (Simple Sequence repeats) primer group based on Fraxinus Velutina Torr. transcriptome sequencing information development and an application of the primer group in germplasm identification, belonging to the technical field of molecular biology. The SSR primer group comprises 42 pairs of primers, wherein the nucleotide sequences of the first pair to the forty-second pair of primers are shown in SEQ ID No.1 to SEQ ID No.84. Experiments verify that the SSR primer group disclosed by the invention has the advantages of rich polymorphism, good repeatability, clear electrophoretic band and the like. The SSR primer group can be effectively used for researches on germplasm identification, DNA fingerprint establishment and the like. A germplasm identification method is sensitive and reliable, can be used for rapidly and accurately realizing and finishing distinguishment and identification of Fraxinus chinensis germplasm, and has important significances in identification and intellectual property protection of Fraxinus chinensis germplasm resources and genetic breeding of genetic breeding molecules.

The invention discloses an alderleafed whitebeam scion grafting method. The alderleafed whitebeam scion grafting method comprises the following steps that the portion, away from the ground by 20-25 cm, of fraxinus chinensis stock is stumped before grafting; one side of the section of the stock is cut once slightly to expose the cambium, and then the portion which is located at the inner side of the cambium and slightly has xylem is vertically cut by 2-3 cm; the side, without buds, of a scion is cut downwards into the bottom in the direction parallel to the scion axis, part of xylem is cut off, the section is 2-3 cm long, and the opposite side of the cut section is cut into a 45-degree small oblique plane with the length of 0.5 cm; the big cut face of the scion is inserted into a stock notch inwards, the cambium of the stock and the cambium of the scion are aligned, the upper end of the scion cut face need to be exposed by about 0.2 cm, the stock and the scion are bound from bottom to top, and a worker should pay attention to the requirement that the grafting portion cannot be exposed. The alderleafed whitebeam scion grafting method is convenient to operate, high in propagation speed, low in cost and short in seedling raising period and saves labor and materials, the grafting survival rate reaches 95% or more, and nursery stock is high in growth speed and good in quality.

The invention provides a tissue culture vegetative propagation method of fraxinus chinensis. The tissue culture vegetative propagation method comprises the following steps: firstly, taking positions of 3cm at root tips of the fraxinus chinensis as tissue culture objects; secondly, uniformly slitting sterilized tissue culture objects into 5 to 6 sections, and inoculating into a culture medium for induced culture of cluster buds; thirdly, cutting off the cluster buds obtained in the second step, taking base parts of the cluster buds by 2 to 3 cm, inoculating into a rooting culture bottle, and sealing for induced rooting; fourthly, growing in the rooting culture bottle until the heights of seedlings reach 6 to 8cm, then opening a bottle cap, hardening seedlings for 4 days, then taking out the tissue culture seedlings, and transplanting in a seedbed culture medium. According to the tissue culture vegetative propagation method provided by the invention, root tips of the fraxinus chinensis are used as explants, and complete regenerated plants can be obtained. For the regenerated plants obtained by the method, botanic characters are stable, a multiplication coefficient reaches 4 to 6, the rooting rate reaches 78 percent, and the transplantation survival rate reaches 97 percent; a rapid propagation method is good in stability and can be used for rapidly propagating the fraxinus chinensis in batches.

The invention discloses a rapid propagation method for fraxinus chinensis. The rapid propagation method comprises the following steps: collecting cuttings; preparing a cutting seedbed: spraying a 0.3% potassium permanganate solution to sterilize a substrate, and putting the sterilized substrate into seedling raising cups, wherein the substrate of the cutting seedbed consists of peat, mushroom residues, mulberry twig powder and vermiculite in a weight ratio of 10:(1-3):(3-5):(4-6); soaking the cuttings in the 0.3% potassium permanganate solution for sterilization, and further soaking in a 2000mg/L indolebutyric acid solution so as to obtain treated cuttings; cutting the treated cuttings into the seedling raising cups, and thoroughly watering the substrate after the cutting depth of the cuttings in the substrate is 3-4cm; and performing cottage management and acclimatization and transplanting after cottage, thereby obtaining fraxinus chinensis seedlings. By adopting the rapid propagation method disclosed by the invention, the rooting rate can be up to 90% or higher after 15-20 days of cottage culture, the whole cottage process only needs 22-30 days, the cottage survival rate can be up to 85%, the rapid propagation method has the advantages of simple and practicable operation, low cost, and the like, and a good basis is made for large-scale and industrial planting of the fraxinus chinensis.

The invention discloses a fraxinus chinensis seedling breeding box capable of achieving layered cultivation. The fraxinus chinensis seedling breeding box capable of achieving layered cultivation comprises a cultivation box, wherein supporting bottom plates are horizontally and fixedly connected to the left side and the right side of the bottom of the cultivation box; hydraulic rods are horizontally and fixedly connected to the left end and the right end of the lower side of a telescopic mechanism; inclined top plates are horizontally and fixedly connected to the telescopic ends of the hydraulic rods; the end faces, close to the inclined top plates, of the supporting bottom plates are inclined sliding faces; and rolling wheels are fixedly connected to the lower sides of the front ends and the rear ends of the inclined top plates. According to the fraxinus chinensis seedling breeding box capable of achieving layered cultivation provided by the invention, high-pressure water pumps are started, liquid in power water boxes is filled into piston cylinders, and piston blocks are jacked upwards by means of hydraulic pressure of the liquid, so that jacking rods drive a planting plate to ascend to the upper layer position of the cultivation box; and in the process, one ends of pull ropes are pulled upwards along with the planting plate, and then the other ends of the pull ropes change the direction through fixed pulleys to pull a box cover obliquely downwards, so that the box cover is rotated to be opened, and the planting plate can conveniently carry the seedlings to be located at an upper opening of the cultivation box and directly make contact with the external natural environment.

The invention discloses a rapid propagation method of excellent male strains of fraxinus chinensis. The method comprises steps as follows: obtaining of fraxinus chinensis explants; disinfection and sterilization of the fraxinus chinensis explants; screening of stem tip and stem segment proliferation media of the fraxinus chinensis; screening of rooting medium of the fraxinus chinensis; domestication and transplanting of fraxinus chinensis test-tube plantlets. With the adoption of the method, propagation coefficient of the male plants of the fraxinus chinensis is greatly increased, seedling cycle is shortened, meanwhile, excellent characteristics of the male plants of the fraxinus chinensis are reserved, excellent effects of stable inheritable characters, high proliferation speed and high propagation coefficient are realized, and technical guarantee and support are provided for large-scale seedling culture of the excellent male strains of the fraxinus chinensis.

The invention provides a saline-alkali land original soil planting method for planting fraxinus chinensis by rooting agents containing raspberry extracts. The method comprises the step of spraying and coating root agents. The root agents are sprayed and coated on a tree pit bottom wall and a side wall, and are prepared from the following ingredients including indoleacetic acid, 6-benzylamino adenine, fulvic acid, soil activators, sustained-releasing agents, caryophyllene, raspberry extracts, pyrite powder, medical stone ball powder, amino acid salt, shikimic acid, biomass extraction liquid, sleeve-fish paste, ferric oxide, anti-blocking agents and aloe. The original soil planting method provided by the invention has the advantages that the newly growing root diameter and the rooting rate are effectively improved; the newly growing root average root diameter reaches 1.6 to 2.3mm; the rooting rate reaches 90.5 to 96.1 percent.