Method for identifying fraxinus chinensis species by virtue of SSR (Simple Sequence Repeat) fingerprint spectrum

Abstract

The invention discloses a method for identifying fraxinus chinensis species by virtue of an SSR (Simple Sequence Repeat) fingerprint spectrum. The method comprises the following steps: (1) extracting total DNA of fraxinus chinensis; (2) performing PCR (Polymerase Chain Reaction) amplification on SSR sequences; (3) electrophoresing PCR amplification products, dyeing and identifying, wherein fraxinus chinensis DNA can be subjected to PCR amplification by virtue of four pairs of SSR primer combinations disclosed in the step (2) to obtain a result that the difference of the amplification products passing through polyacrylamide gel electrophoresis detection strips is remarkable, and then the SSR fingerprint spectrum of the fraxinus chinensis species is built. According to the method, fraxinus chinensis species resources are accurately identified in molecular level, and the problem of difficult identification of the fraxinus chinensis species is solved; moreover, the method is short in detection time, good in repeatability and high in accuracy, and has specificity in collected 12 species.

Description

technical field [0001] The invention relates to a method for identifying white wax species by using SSR fingerprints, and belongs to the field of biotechnology. Background technique [0002] One of the most important applications of fingerprints is the identification of germplasm. Unclear identification of germplasm will lead to more inferior species, waste of germplasm resources, and even population degradation. Therefore, germplasm identification is very important both in production and research. The traditional identification method is complex, the identification period is long, and it is easily affected by various factors such as sampling site, developmental period, and environment, and the accuracy rate is not high. The identification efficiency is low. Especially in the identification of plant seedlings, it is difficult to distinguish between different provenances, clones, and varieties within related species. The fingerprint technology developed in recent years is...

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Problems solved by technology

At the same time, the marker technology used needs to be highly sensitive, and the markers obtained need to be individual-specific. At present, there are very few studies on the molecular level of white wax. Wang Jianbing et al. optimized the SSR-PCR reaction system of white wax and screened primers (2014) , but there are few reports on the practical application and research on the SSR fingerprint of white wax

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