Tissue culture vegetative propagation method of fraxinus chinensis

Abstract

The invention provides a tissue culture vegetative propagation method of fraxinus chinensis. The tissue culture vegetative propagation method comprises the following steps: firstly, taking positions of 3cm at root tips of the fraxinus chinensis as tissue culture objects; secondly, uniformly slitting sterilized tissue culture objects into 5 to 6 sections, and inoculating into a culture medium for induced culture of cluster buds; thirdly, cutting off the cluster buds obtained in the second step, taking base parts of the cluster buds by 2 to 3 cm, inoculating into a rooting culture bottle, and sealing for induced rooting; fourthly, growing in the rooting culture bottle until the heights of seedlings reach 6 to 8cm, then opening a bottle cap, hardening seedlings for 4 days, then taking out the tissue culture seedlings, and transplanting in a seedbed culture medium. According to the tissue culture vegetative propagation method provided by the invention, root tips of the fraxinus chinensis are used as explants, and complete regenerated plants can be obtained. For the regenerated plants obtained by the method, botanic characters are stable, a multiplication coefficient reaches 4 to 6, the rooting rate reaches 78 percent, and the transplantation survival rate reaches 97 percent; a rapid propagation method is good in stability and can be used for rapidly propagating the fraxinus chinensis in batches.

Description

technical field [0001] The invention relates to the technical field of artificial breeding of ash evergreen, in particular to a method for asexual reproduction of ash evergreen through tissue culture. Background technique [0002] Evergreen ash is a species of light-loving trees in warm temperate zones. The evergreen ash wood is tough and can be used to make furniture, farm tools, vehicles, plywood, etc.; Evergreen ash is a multifunctional tree species in landscaping. It is resistant to cold and drought, salt and alkali, and barrenness. It is a rare evergreen broad-leaved tree species in the northern region, and the market reflects it strongly. Its promotion and application prospects will be very impressive. According to its biological characteristics such as resistance to pruning and shaping and the configuration requirements of scenic spots, it can be built into spherical, single-stem-shaped balls, shrub-like high hedges, column-shaped high hedges, street trees, etc. It...

AI Technical Summary

Problems solved by technology

[0004] At present, evergreen ash cutting propagation is easily affected by cutting substrates, temperature and humidity, and plant growth regulators. The roots of cutting seedlings are adventitious roots with poor absorption capacity. Now the common propagation method of evergreen white wax, but the grafting method is easily restricted by seasons and regions, the operation is complicated, the reproduction coefficient is low, the growth speed of seedlings is different, the management is complicated, and it is not suitable for batch and large-scale reproduction.