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Method for tissue culture and rapid breeding of Fraxinus chinensis

A tissue culture, ash tree technology, applied in horticultural methods, botanical equipment and methods, climate change adaptation and other directions, can solve the problems of long cycle, low efficiency, inability to meet the greening process, etc., and achieve good quality of seedlings and fast reproduction. Effect

Active Publication Date: 2015-03-11
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0004] There are large areas of saline-alkali land in my country's coastal areas, and the number of seedlings required for greening is huge. However, the current breeding method of ash, which is an excellent salt-resistant plant, has a long cycle and low efficiency, and cannot meet the needs of the greening process. This problem needs to be solved urgently

Method used

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Embodiment

[0029] The ash tree used for breeding and drawing materials can be a healthy plant growing in the wild, such as the ash tree that grows in the heavy saline-alkali soil of Haixing County, Cangzhou City, Hebei Province. Take the ash tree with a tree age of 8 years as an example, select the new shoots that grew in that year, remove Extra blades and unsuitable parts.

[0030] A. Material disinfection: Take the axillary buds or stem tips of the ash tree with growth points, the length can be selected as 2-3cm, rinse with clean flowing water for 2-4 hours, then use filter paper to absorb the water attached to the surface, and use 75% Disinfect the surface with alcohol for 20-30 seconds, then disinfect with 5% sodium hypochlorite for 20-25 minutes, and rinse with sterile water for 5-6 times;

[0031] B, start culture: absorb the water attached to the surface of the sterilized material in step A with sterilized filter paper, then use a scalpel (12 cm long, blades can be replaced) to cu...

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Abstract

The invention discloses a method for the tissue culture and the rapid breeding of Fraxinus chinensis. According to the method, axillary buds or stem tips of Fraxinus chinensis are propagated into nursery seedlings through several steps: material disinfection, startup culture, proliferation and differentiation culture, rooting culture, transplantation, strong seedling culture and the like, and the survival rate of the transplantation of test-tube seedlings is over 90%. The method has the advantage of high breeding speed, so that breeding can be carried out annually, the breeding process of the Fraxinus chinensis can be significantly accelerated, and the problem of shortage of green plant seedling sources for large area afforestation on saline-alkali lands in China is effectively solved. The method can also greatly reduce the cost for afforestation and save public resources.

Description

technical field [0001] The invention relates to a method for breeding trees, in particular to a method for cultivating and breeding ash trees. Background technique [0002] Ash tree (Fraxinus chinensis), also known as green palm tree and white wattle tree, is a deciduous tree belonging to the genus of Oleaceae. There are about 70 species in the world, and there are more than 20 species in my country. Most of them are timber species and landscaping species, mainly distributed in temperate zones area. Ash tree has luxuriant branches and leaves, well-developed root system, rapid growth, straight trunk, beautiful tree shape, strong adaptability, good salt resistance, and can be planted in saline-alkali land. It is an important tree species for saline-alkali landscaping. [0003] For a long time, ash trees have been mainly propagated by seeds. Due to their long juvenile period and obvious fruiting interval, the reproductive cycle is long. Not only that, but there are many disadva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 王玉珍刘小京张秀梅封晓辉谢志霞
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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