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SSR primer group for analyzing Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure, and application of SSR primer group

A technology of powdery mildew of rubber tree and population genetics, applied in the direction of proteomics, microbe-based methods, microorganisms, etc., can solve the problems of no SSR marker research reports on powdery mildew of rubber tree, achieve good repeatability, high polymorphism, Effects of genome wide distribution

Active Publication Date: 2021-12-07
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to its co-dominant inheritance, high polymorphism, good repeatability, and widespread genome distribution, it has become one of the most widely used molecular markers in the analysis of the genetic structure of plant pathogen populations. Tagged Research Reports

Method used

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  • SSR primer group for analyzing Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure, and application of SSR primer group
  • SSR primer group for analyzing Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure, and application of SSR primer group
  • SSR primer group for analyzing Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure, and application of SSR primer group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 SSR primer development

[0027] (1) Genomic SSR locus analysis

[0028] Download the whole genome sequence of rubber tree powdery mildew fungus from the NCBI (National Center for Biotechnology Information) website, use MISA software to search for SSR sites of 2 to 6 nucleotides in the genome sequence, and the search condition is the number of repetitions of 2 nucleotides Must be ≥6, and the number of repetitions of 3-6 nucleotides must be ≥5.

[0029] A total of 2720 SSR sites meeting the search criteria were found, among which dinucleotide SSR sites were the most, 1619; followed by trinucleotide SSR sites, 682; they respectively accounted for 59.52 of the total number of SSR sites and 25.07%; there are 330 tetranucleotide SSR sites, accounting for 12.13%; and the five and six nucleotide SSR sites are not more than 50, accounting for less than 2%.

[0030] Table 1. Distribution of SSRs in the Genome of Powdery mildew Hevea

[0031]

[0032] (2) SSR pr...

Embodiment 2

[0053] Embodiment 2, utilize SSR primer to carry out population genetic structure analysis to Hevea powdery mildew in different areas

[0054] (1) 15 powdery mildew strains (Table 6) were obtained from Guangdong, Yunnan and Hainan respectively, and after DNA was extracted with the Tiangen Plant Genome Extraction Kit, PCR amplification was performed. The specific operation was the same as in Example 1.

[0055] Table 6. Information of 45 strains of powdery mildew of rubber tree

[0056]

[0057]

[0058] (2) DNA sequencer ABI3730x1 was used to perform capillary fluorescence electrophoresis detection on the amplified products, and GeneMarker V3.0.0 was used to read the capillary electrophoresis data, and the detection results were counted (Table 7).

[0059] Table 7. The amplification results of 16 pairs of SSR primers in 45 Hevea powdery mildew

[0060]

[0061] (3) Using GenAlEx 6.5 software to calculate the observed allele number Na, effective allele number Ne, Shan...

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Abstract

The invention discloses an SSR primer group for analyzing a Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure, and application of the SSR primer group. The SSR primer group for analyzing the Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure comprises 16 pairs of primers, wherein the nucleotide sequences of the primers are as shown in SEQ ID NO.17-48. The invention also provides application of the primer group in the Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure analysis. The primer group has the characteristics of being good in specificity, stable in amplification, rich in polymorphism, good in repeatability, convenient in counting and the like, and a new technology and a new means are provided for research on the Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. powdery mildew colony genetic structure.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an SSR primer set for analyzing the genetic structure of powdery mildew of rubber tree populations and an application thereof. Background technique [0002] Natural rubber is an important national strategic material, and it is also one of the objects in the national grain production functional area and important agricultural product production protection area. Powdery mildew caused by obligate parasitic rubber tree powdery spore (Oidium heveae Steinm) is one of the most serious diseases on rubber trees in my country. The spring when the rubber tree draws young leaves in large quantities. Once the disease breaks out, it will cause the new leaves of the gum tree to fall off in a large area, the growth of the gum tree will be weak, and the new shoots will die, which will seriously affect the latex production and delay the cutting time of the gum tree. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11G16B20/30C12R1/645
CPCC12Q1/6895C12Q1/6858G16B20/30C12Q2600/156C12Q2531/113C12Q2525/151C12Q2565/125C12Q2563/107
Inventor 曹学仁车海彦罗大全韩乔慧何永祥
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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