ssr primer set for analysis of genetic structure of powdery mildew of rubber tree and its application

A technology of powdery mildew of rubber tree and population genetics, applied in the direction of proteomics, microbe-based methods, microorganisms, etc., can solve the problems of SSR marker research reports on powdery mildew of rubber tree, and achieve rich polymorphism, easy statistics, and expansion The effect of increasing stability

Active Publication Date: 2022-07-26
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

Due to its co-dominant inheritance, high polymorphism, good repeatability, and widespread genome distribution, it has become one of the most widely used molecular markers in the analysis of the genetic structure of plant pathogen populations. Tagged Research Reports

Method used

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  • ssr primer set for analysis of genetic structure of powdery mildew of rubber tree and its application
  • ssr primer set for analysis of genetic structure of powdery mildew of rubber tree and its application
  • ssr primer set for analysis of genetic structure of powdery mildew of rubber tree and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 SSR primer development

[0027] (1) Genomic SSR locus analysis

[0028] Download the whole genome sequence of P. rubra from the NCBI (National Center for Biotechnology Information) website, and use MISA software to search for SSR sites of 2 to 6 nucleotides in the genome sequence, and the search condition is the number of repetitions of 2 nucleotides It must be ≥6, and the number of repetitions of 3-6 nucleotides must be ≥5.

[0029] A total of 2,720 SSR sites were found that met the search criteria, of which dinucleotide SSR sites were the most, with 1,619 sites; followed by tri-nucleotide SSR sites, with 682 sites; they accounted for 59.52 of the total SSR sites. and 25.07%; there were 330 tetranucleotide SSR sites, accounting for 12.13%; and neither pentanucleotide nor hexanucleotide SSR sites exceeded 50, accounting for less than 2%.

[0030] Table 1. Distribution of SSRs in the Genotypic SSR of Helicobacter

[0031]

[0032] (2) SSR primer design an...

Embodiment 2

[0053] Embodiment 2, utilizes SSR primer to carry out population genetic structure analysis to rubber tree powdery mildew in different regions

[0054] (1) 15 powdery mildew strains (Table 6) were obtained from Guangdong, Yunnan and Hainan respectively, and after DNA was extracted with the Genome Extraction Kit of Tiangen, PCR amplification was performed, and the specific operation was the same as that in Example 1.

[0055] Table 6. Information on 45 rubber tree powdery mildew strains

[0056]

[0057]

[0058] (2) Capillary fluorescence electrophoresis was used to detect the amplified products using DNA sequencer ABI3730x1, and GeneMarker V3.0.0 was used to read the capillary electrophoresis data, and the detection results were counted (Table 7).

[0059] Table 7. Amplification results of 16 pairs of SSR primers in 45 rubber tree powdery mildews

[0060]

[0061] (3) GenAlEx 6.5 software was used to calculate the observed allele number Na, effective allele number N...

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Abstract

The invention discloses an SSR primer set for analyzing the genetic structure of the rubber tree powdery mildew population and its application. The SSR primer set for analyzing the genetic structure of the rubber tree powdery mildew population includes 16 pairs of primers, and the nucleotide sequences of the primers are shown in SEQ ID NO. 17-48. The invention also provides the application of the primer set in the analysis of the genetic structure of the rubber tree powdery mildew population. The primer set has the characteristics of good specificity, stable amplification, rich polymorphism, good repeatability, convenient statistics, etc., and provides a new technology and means for the research on the genetic structure of the rubber tree powdery mildew population.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an SSR primer set used for the analysis of the genetic structure of the rubber tree powdery mildew population and its application. Background technique [0002] Natural rubber is an important national strategic material, and it is also one of the objects in the national food production functional area and important agricultural product production protection areas. Powdery mildew caused by the obligate parasitic Oidium heveae Steinm is one of the most serious diseases on rubber trees in my country. The spring of the rubber tree with a lot of young leaves. Once the disease breaks out, it will cause the new leaves of the gum tree to fall off in a large area, the growth of the gum tree will be weak, and the new shoots will die, which will seriously affect the latex yield and delay the cutting time of the gum tree. [0003] By studying the population genetic str...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11G16B20/30C12R1/645
CPCC12Q1/6895C12Q1/6858G16B20/30C12Q2600/156C12Q2531/113C12Q2525/151C12Q2565/125C12Q2563/107
Inventor 曹学仁车海彦罗大全韩乔慧何永祥
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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