Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis

A quality control method and technology of Cordyceps fungus powder, which can be applied to plant raw materials, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve problems such as not seen, and achieve simple operation, strong specificity, and good repeatability. Effect

Active Publication Date: 2008-10-29
SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the analysis of the fingerprint of Cordyceps sinens...

Method used

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  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis
  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis
  • Fingerprint pattern quality control method for cordyceps sinensis bacterium powder raw material in herbs medicaments for strengthening the body resistance and activating blood and dissolving stasis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Fingerprint test method

[0060] 1. Reagents and medicinal materials

[0061] Purified water (Shui Sen live, Coca-Cola Company); Acetonitrile (chromatographically pure, Tedia, the United States); Adenosine (China Pharmaceutical and Biological Products Identification Institute, for content determination, batch number: 879-200202); Uridine (China Pharmaceutical and Biological Products Identification Institute, for identification, batch number: 887-200202); guanosine (purchased from Xinxiang Tuosheng Chemical Technology Co., Ltd.); Cordyceps mycelium powder (produced by the present invention).

[0062] 2 experimental equipment

[0063] HP1100 Liquid Chromatograph (DAD Diode Array Detector, Agilent, USA); Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd., KQ-50E); Chromatographic Column Thermostat (Tianjin Hengao Technology Development Co., Ltd., HT-130 ); HPLC membrane (Jiangsu Hanbang Technology Co., Ltd., 0.45um)

[0064] 3 Extraction of Cordyceps mycelium p...

Embodiment 2

[0071] Take 10mL primary seeds into a 500mL Erlenmeyer flask containing 100mL secondary seed medium, rotating speed: 150rpm, pH6.2-6.8; cultivate under aerobic conditions at 28°C for 2-5 days; Strain, to start large-scale Cordyceps fermentation with fermenter; use fermentation medium in fermenter, rotating speed 150-250rpm; cultivate at 28°C dissolved oxygen concentration: 20-90 for 2-5 days, pH 6.2-6.8; The culture medium is removed, and the mycelium is dried to obtain the product Cordyceps fungus powder.

[0072] The seed medium is: glucose 2%, peptone 1%, ammonium nitrate 0.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, ferrous sulfate 0.001%, sodium chloride 0.1%, bran 5%;

[0073] The fermentation medium is: glucose 2%, peptone 1%, ammonium nitrate 0.5%, magnesium sulfate 0.05%, dipotassium hydrogen phosphate 0.1%, ferrous sulfate 0.001%, sodium chloride 0.1%, 5% silkworm chrysalis powder 5%;

[0074] Fermentation medium, 5% bran, 5% silkworm chrysalis...

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PUM

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Abstract

The invention relates to a control method of the fingerprint spectrum quality of cordyceps sinensis powder raw material in botanical drug for strengthening vital qi and removing blood stasis, comprising the steps that: (1) cordyceps sinensis powder is extracted: 0.100g of cordyceps sinensis powder is taken, purified water is added, the ultrasonic extraction, the filtration and the sample injection are carried out; (2) the gradient elution with mobile phase is carried out: octadecyl silane bonded silica gel is taken as a filler, water and acetonitrile are taken as mobile phase to carry out the gradient elution for 0 to 30min and 0 to 7 percent B; (3) a standard fingerprint spectrum is established: the HPLC standard fingerprint spectrum of the cordyceps sinensis powder is determined, and 3 characteristic peaks are selected; (4) the quality control of the fingerprint spectrum is carried out: the relative retention time of No.2 peak uridine, No.3 peak guanosine and No.4 peak adenosine are 0.44 plus or minus 0.03, 0.68 plus or minus 0.03 and 1.00 respectively; the HPLC fingerprint spectrum of the sample is compared with the contrast fingerprint spectrum. The similarity calculated by the 5 common peaks is not less than 0.9, (5) the preparation of the cordyceps sinensis powder raw material is carried out; the control method has good repetitivity and can fully reflect the basic characteristics of nucleoside ingredients of the cordyceps sinensis powder.

Description

technical field [0001] The invention belongs to the field of quality control methods for raw materials of Cordyceps fungus powder, and in particular relates to a method for quality control of fingerprints of raw materials of Cordyceps fungus powder in herbal medicines for strengthening health and removing blood stasis. Background technique [0002] Cordyceps sinensis is a compound of the subunits and larvae corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of bat moths. It is a famous and precious traditional Chinese medicine for a long time. It is distributed in Qinghai, Tibet, Sichuan and other places in China, and exists in grassy soils in mountainous areas at an altitude of 3000-4000m in Northwest and Southwest China. The part exposed on the soil surface is the fruiting body of the fungus, 3-6cm long, black in color, and shaped like a blade. The body part buried in the soil is 2.5-4m long, yellow to dark brown. Because its shape changes uniquely...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61P15/10A61P11/00A61P29/00G01N30/02G01N30/86C12N1/14
Inventor 胡坪潘一峰
Owner SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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