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Methods for establishing fingerprints of cistanche extracts and identifying quality of drugs

A technology of Cistanche deserticola extract and fingerprint spectrum, which is applied in the identification of the quality of Cistanche deserticola medicinal materials, the establishment of high performance liquid chromatography fingerprints of Cistanche deserticola extracts, and the establishment of fingerprints of Chinese medicinal materials, and can solve the problems of lack of universality, few chromatographic peaks, and separation. problems such as poor conditions, to achieve the effects of good stability and reproducibility, easy production and easy realization of chromatographic conditions

Inactive Publication Date: 2010-05-05
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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AI Technical Summary

Problems solved by technology

There are obvious deficiencies in several literatures on fingerprint research. Xia Pengxiao et al. published a paper titled "HPLC Fingerprint Research of Cistanche" in the fifth issue of "Chinese Drug Application and Monitoring" in 2006. Chromatographic elution, the separation is not good, especially within 10min, there are a large number of overlapping peaks; they also published a paper entitled "Research on the HPLC Fingerprint of Cistanche" in the 4th issue of "Pharmaceutical Services and Research" in 2008, Changing to binary gradient for chromatographic elution, the separation effect is significantly improved, but there are still a lot of overlapping phenomena in the areas of 7-9min, 11-18min and 21-23min. In the paper titled "Research on the HPLC Fingerprint of Desert Cistanche" published by Xie Jiena et al. in the second issue of "Chinese Herbal Medicine" in 2005, although medicinal materials from different origins were selected, the number of chromatographic peaks was small and there were common characteristic peaks. less (only two)

Method used

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  • Methods for establishing fingerprints of cistanche extracts and identifying quality of drugs
  • Methods for establishing fingerprints of cistanche extracts and identifying quality of drugs
  • Methods for establishing fingerprints of cistanche extracts and identifying quality of drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Instruments and medicines: Shimadzu LC20A high performance liquid chromatography was used; 15 batches of Cistanche medicinal materials were purchased from Chinese herbal medicine stores from Inner Mongolia, Xinjiang, Gansu, Ningxia, Qinghai and other places; other reagents were chromatographically pure or analytically pure .

[0023] (2) Preparation of the test solution: Accurately weigh 2.0000 g of dried Cistanche medicinal material powder, place in a stoppered container, add 120 mL of methanol, stopper and soak for 2 hours, ultrasonically shake for 40 minutes, transfer to a Soxhlet extractor, and reflux Extract for 3 hours, concentrate the extract to less than 25 mL, filter through a 0.45 μm microporous membrane, and dilute the filtrate to 25 mL with methanol;

[0024] (3) HPLC analysis: the chromatographic separation column filler is 5 μm Kromasil-C18, 250mm × 4.6mm, the eluent phase A is 0.15% phosphate buffer solution (pH=7.7), and phase B is methanol-acetonitr...

Embodiment 2

[0028] (1)~(4) steps are identical with embodiment 1;

[0029] (5) get a kind of Cistanche medicinal material that needs to be identified and prepare the sample solution by the same dosage and process as the preparation method of the test solution in (2), and carry out separation and analysis under the chromatographic conditions listed in (3) to obtain fingerprints, such as image 3 As shown, compared with the standard fingerprint spectrum determined in (4), check the situation of common characteristic peaks, find that the similarity (retention time and peak area) of its common peaks is less than 60%, show that this medicinal material is unqualified Cistanche deserticola herbs.

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Abstract

The invention provides methods for establishing fingerprints of cistanche extracts and identifying quality of drugs, comprising preparation of test solution, facture of fingerprints, determination of standard fingerprints and identification of cistanche drugs. HPLC fingerprints are established for methanol extracts of the cistanche drugs with different places of production, different quality or different storage time, and 21 common characteristic peaks are determined through analysis and comparison and form the fingerprint characteristics of the cistanche drugs, and the fingerprint characteristics can be used as the standard fingerprints of the cistanche drugs. The fingerprints of the cistanche drugs needing to be identified can be compared with the standard fingerprints and the common characteristic peaks are detected to identify the quality of the cistanche drugs. The methods are characterized by simpleness and convenience, good reproducibility, multiple characteristic peaks, accuracy, reliability and the like, and ensure the quality control of the cistanche drugs to be more perfect and scientific.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing high-performance liquid chromatography fingerprints of Cistanche deserticola extracts, and a method for identifying the quality of medicinal materials Cistanche deserticola using the fingerprints as a standard. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. At present, fingerprints have become one of the most effective means to identify the varieties of traditional Chinese medicines and evaluate the quality of traditional Chinese medicines recognized at home and abroad. In the "FDA ...

Claims

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Application Information

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IPC IPC(8): G01N30/36G01N30/86A61K36/64
Inventor 周考文杨烨周璐
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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