Method for constructing hepatoma protein group fingerprint model and serum detection application thereof

A proteome and fingerprint technology, applied in the field of malignant tumor detection, can solve the problems of undetectable small liver cancer, and achieve the effects of easy detection, good repeatability, and prolonged survival

Inactive Publication Date: 2005-08-17
郭爱林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot detect small liver cancers less than 2 cm
And the test results must be verified by AFP test

Method used

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  • Method for constructing hepatoma protein group fingerprint model and serum detection application thereof
  • Method for constructing hepatoma protein group fingerprint model and serum detection application thereof
  • Method for constructing hepatoma protein group fingerprint model and serum detection application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 A serum proteome fingerprint model and construction method for detecting liver cancer according to the present invention:

[0030] 1. Serum Pretreatment

[0031] Serum samples were thawed in an ice bath and centrifuged at 3000r / min for 5 minutes, 6 μl of serum was added to 18 μl of urea, mixed thoroughly, ice bathed for 30 m, and diluted to 120 μl with WCX2 binding buffer.

[0032] 2. Pretreatment of WCX-2 protein chips

[0033] Pretreat the WCX-2 protein chip with 5 μl of 10 mmol / L HCI for 10 min, wash the chip with M-Q water for 3 times, install the chip on the Bioprocessor, add 150 uL of binding buffer (50 mmol / L sodium acetate, pH 4.0) to each point of the chip, Shake and incubate at room temperature for 5 min, discard the liquid, and repeat 1 time.

[0034] 3. Combination of chips and samples

[0035] Add 100 μl of the diluted sample to the chip and incubate with shaking. Binding buffer was added for elution twice in a row, 5 min each time. The Biopr...

Embodiment 2

[0042] Embodiment 2 Partial examples

[0043] 1. Reagents and chips

[0044] Acetonitrile, trifluoroacetic acid, urea, protease inhibitors, Hepes, Tris-HCl, CHAPS and OGP were purchased from Sigma. WCX2 protein chips were purchased from Ciphergen, USA. Protein standard Aβ-(1-42) was purchased from Sigma.

[0045] 2. Specimen source

[0046] Among the 160 serum samples, the serum samples of 60 patients with hepatocellular carcinoma were from newly diagnosed patients admitted to the surgical department of major hospitals in Guangzhou from August 2002 to August 2003; Volunteer healthy people, observed for half a year and no tumor occurred. 60 cases of liver cancer were diagnosed as hepatocellular carcinoma by postoperative pathology. There were 85 males and 15 females with hepatocellular carcinoma, aged from 32 to 75 years old, with an average of 59.6 years old. According to the "Clinical Diagnosis and Staging Standards for Primary Liver Cancer" of the Liver Cancer Professi...

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Abstract

This invention provides a non-invasion for testing liver cancer, which sets up a liver cancer protein set fingerprint atlas model used in the serum test. Said model is composed of a HAS set mass spectrum atlas and an artificial nerve net analysis method used in early serology test. The atlas is composed of the protein with five M/Z. The method for setting up the atlas model includes: 1, preparation of serum, 2, mass spectrum test and collection of data for the serum specimen, 3, artificial nerve net analysis of the spectrum data, which can test the liver cancer before there is not any pathologic change appearing to the cell.

Description

Technical field: [0001] The invention relates to the field of malignant tumor detection, and is a new non-invasive detection method, which uses human serum to detect liver cancer in vitro, and the sensitivity and specificity of the early detection can reach more than 90%. Background technique: [0002] Primary liver cancer is one of the most common clinical malignant tumors, with about 260,000 cases occurring every year in the world, of which 42% are in China. According to the statistics of the Ministry of Health, in the past 20 years, about 110,000 people died of this disease every year in my country, accounting for the second place in the death rate of malignant tumors in some cities and the first place in some rural areas. Since the onset of liver cancer is mostly hidden and the early symptoms are not obvious, once clinical symptoms appear, it is mostly in the middle and late stage, so the opportunity for treatment is often missed. Early detection, early diagnosis, and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/00G01N30/86G01N30/88G01N33/48
Inventor 郭爱林
Owner 郭爱林
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