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Reagent for detecting multiple myeloma characteristic protein by mass spectrum

Inactive Publication Date: 2009-01-28
许洋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2-DE was first used in clinical proteomics research, but its resolution for hydrophobic, strongly acidic and strongly basic proteins is not enough, and it cannot detect low-abundance proteins, so its practical value is limited
[0007] However, there has been no report on the use of magnetic beads to capture biomarkers and the use of mass spectrometry to detect serum characteristic proteins of multiple myeloma

Method used

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  • Reagent for detecting multiple myeloma characteristic protein by mass spectrum
  • Reagent for detecting multiple myeloma characteristic protein by mass spectrum
  • Reagent for detecting multiple myeloma characteristic protein by mass spectrum

Examples

Experimental program
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Embodiment 1

[0075] Example 1 The distinction between normal and multiple myeloma patients and the preparation of mass spectrometry kits

[0076] (1) Experimental method

[0077] A total of 54 consecutive patients with multiple myeloma (37 males and 17 females) were used in this study. Their average age was 58 (range 46 to 83). 25 patients were newly diagnosed, according to the Durie / Salmon staging system, 7 of them were multiple myeloma stage II, 18 were stage III, 29 underwent chemotherapy after blood samples were collected (6 achieved complete remission , 13 partial responses, 5 relapses, and 5 other conditions). Among the 29 patients who underwent chemotherapy, 13 were selected for the second chemotherapy after 2 or 3 sampling chemotherapy. Blood samples from these patients were collected from serum. 108 non-multiple myeloma control serum samples from healthy volunteers (n=62) or patients with other diseases that must be differentiated from multiple myeloma (POEMS syndrome, n=4); N...

Embodiment 2

[0097] Embodiment 2 clinical trial and double-blind test;

[0098] Since the combination of multiple characteristic proteins can completely separate multiple myeloma from normal people, any two or more of the above-mentioned 56 characteristic proteins are selected, and according to the mass-to-charge ratio m / Based on the z value and the critical peak value M of the protein, patients with multiple myeloma were identified from normal people and multiple myeloma (POEMS syndrome; NHL; MGUS; CRF; rheumatism; macroglobulinemia ; or other tumors with bone metastases) two-to-two identification of serum signature protein detection mass spectrometry model ( figure 1-5), said specific protein mass-to-charge ratio m / z and critical peak value M are respectively m / z=8139, M≤5.88; m / z=11682, M≤0.78; m / z=22778, M≤ 0.64 ((The upper part of Figure 2(a), (b), (c) is the calibration map representing MM (marked as MM1 and MM2); the middle part of Figure 2(a), (b), (c) is the non- MM control [di...

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Abstract

The invention builds a blood serum protein mass spectrometric model that is used for detecting multiple myeloma, finds out that the blood serum protein of multiple myeloma patients has 56 characteristic proteins, chooses any two or over two proteins from the 56 characteristic proteins, and uses mass spectrometric analysis, thus detecting diseases including the multiple myeloma. According to the mass spectrometric model, the invention develops a reagent and a kit that are used for detecting the characteristic proteins in a biological sample through a mass spectrometric method.

Description

Technical field: [0001] The invention relates to a detection reagent for multiple myeloma protein, in particular to a reagent for detecting multiple myeloma characteristic protein by mass spectrometry. Background technique: [0002] Multiple myeloma (MM) is a clonal B-cell disorder in which malignant plasma cells (PCs) accumulate in the bone marrow resulting in osteolytic lesions and excessive production of monoclonal proteins. Although substantial progress has been made in the treatment of multiple myeloma, prevention and early detection are still an effective way to reduce the mortality of multiple myeloma and the key to improving the survival rate of patients. [0003] The sensitivity and specificity of current laboratory testing methods for screening multiple myeloma are low, which has become a bottleneck in the early detection of multiple myeloma. Moreover, these methods are usually invasive and expensive, so a highly sensitive, specific, non-invasive, cheap and conven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N30/02
Inventor 王清涛陈文明梁玉芳翟玉华赵冠飞许洋
Owner 许洋
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