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Establishment method of Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum

A technology of fingerprints and fenugreek leaves, which is applied in the field of establishment of HPLC fingerprints of fenugreek leaves, can solve the problems that pharmacological research is only in its infancy, and achieve simple preparation, good stability and reproducibility, and easy chromatographic conditions Effect

Inactive Publication Date: 2015-04-29
DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] At present, the development and utilization of A. chinensis resources are limited to the research on its medicinal parts (including the resinous heartwood), while the leaves of A. chinensis are the most abundant resource parts of the plant, and their pharmacological research is only in its infancy.

Method used

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  • Establishment method of Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum
  • Establishment method of Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum
  • Establishment method of Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum

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Embodiment 1

[0042] Embodiment 1 Method for establishing the fingerprints of the leaves of Akiras sinensis

[0043] (1) Preparation of reference substance solution

[0044] Precisely weigh 12.7 mg of mangiferin reference substance, put it in a 50ml volumetric flask, add methanol to ultrasonically dissolve and dilute to the mark, shake well, and prepare a mangiferin reference substance solution with a concentration of 0.254 mg / ml. After sealing with sealant, store in a refrigerator at 4°C and store away from light.

[0045] (2) preparation of need testing solution

[0046] Precisely weigh 3g of dry Agarwood powder (collected from the standardized planting base of Agarwood, Dongguan City, Guangdong Province, batch number: 201208), put it in a 150ml Erlenmeyer flask, add 50ml of methanol, ultrasonicate for 30min, after cooling, suction filter, and the filtrate is decompressed Concentrate until there is no alcohol smell, extract three times with diethyl ether and water-saturated n-butanol in...

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Abstract

The invention relates to the field of the quality control technology of traditional Chinese medicinal materials and in particular discloses an establishment method of an Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum. The establishment method comprises the steps of preparing reference solution, preparing test article solution, determining the chromatographic conditions of the fingerprint spectrum, and determining a standard fingerprint spectrum; the establishment method is characterized in that the chromatographic conditions of the fingerprint spectrum are as follows: a C18 chromatographic column is adopted, acetonitrile-0.1-0.5% of phosphate buffer solution is used as mobile phase for gradient elution; an ultraviolet detector is used for detection and the detection wavelength is 300-360nm. The fingerprint spectrum has strong characteristic feature, main common peaks can achieve good baseline separation, a test article can be prepared simply and conveniently, the chromatographic conditions can be realized easily, and the method is simple and convenient, has good stability and good reproducibility and is suitable for the quality control of Aquilaria sinensis leaves.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicinal materials, in particular to a method for establishing an HPLC fingerprint of arborina japonica leaves. Background technique [0002] The use of chromatographic fingerprints to obtain comprehensive characteristic information of chemical components of traditional Chinese medicines has been widely used in the quality control of traditional Chinese medicines. Chromatographic fingerprints of traditional Chinese medicines are the expression and reflection of the overall chemical characteristics of traditional Chinese medicines. At present, the US Food and Drug Administration (FDA), the British Herbal Pharmacopoeia, the German Society of Medicinal Botany, the Indian Herbal Pharmacopoeia, and the Canadian Society of Medicinal Botany all accept and record the quality control methods of fingerprints. At present, fingerprints have become internationally recognized. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/34
Inventor 易智彪肖炜华曾宝谢友良马麟王环詹丽玲陈志维赖小平
Owner DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE
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