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Radix salvia miltiorrhiza fingerprint establishment method and standard fingerprint

A technique of fingerprinting and establishing methods, which is applied in the field of drug analysis, can solve problems such as inability to effectively reflect and control the overall quality of traditional Chinese medicine, and achieve good stability and reproducibility

Inactive Publication Date: 2013-02-13
哈药集团中药二厂
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the overall quality of the traditional Chinese medicine cannot be effectively reflected and controlled in the substance identification and component analysis of the existing traditional Chinese medicine, thereby providing a method for establishing the fingerprint of Danshen medicinal material and its standard fingerprint

Method used

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  • Radix salvia miltiorrhiza fingerprint establishment method and standard fingerprint
  • Radix salvia miltiorrhiza fingerprint establishment method and standard fingerprint
  • Radix salvia miltiorrhiza fingerprint establishment method and standard fingerprint

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Experimental program
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specific Embodiment approach 1

[0016] Specific implementation mode one: the method for establishing the fingerprint of the salvia miltiorrhiza medicinal material of the present embodiment is realized according to the following steps:

[0017] 1. Preparation of the test solution: according to the mass volume ratio of 1g: 100 ~ 300mL, mix the Danshen medicinal material powder with water evenly, heat and reflux for 45 ~ 60min, after cooling, take the supernatant and filter it with a microporous membrane, and the filtrate is used as the Test solution;

[0018] Two, high performance liquid chromatography analysis: the need testing solution that step one obtains is injected high performance liquid chromatography, is that 1% glacial acetic acid aqueous solution is A phase with mass percentage composition, and acetonitrile is B phase and carries out mobile phase gradient elution, Analyze the solution of the test product to obtain the fingerprint of the salvia miltiorrhiza; wherein, the high-performance liquid chrom...

specific Embodiment approach 2

[0019] Embodiment 2: This embodiment differs from Embodiment 1 in that the microporous membrane filtration described in step 1 is filtered by a 0.45 μm microporous membrane. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in the mobile phase gradient elution process described in step two, the phase change of eluent A and B is: use gradient elution mode 0min~ 50min, acetonitrile 0% ~ 30%. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a traditional Chinese medicine (TCM) fingerprint establishment method, in particular to a radix salvia miltiorrhiza high-performance liquid chromatograph (HPLC) fingerprint establishment method and a radix salvia miltiorrhiza standard fingerprint obtained by adopting the method, provides a TCM fingerprint establishment method and a standard fingerprint, and aims to solve the problem that TCM substance identification and composition analysis can not effectively reflect and control the total body mass of TCM at present. The method comprises the following steps: firstly, preparation of a test solution; and secondly, high-performance liquid chromatograph analysis. The radix salvia miltiorrhiza fingerprint can be obtained by adopting the method. The method is used in the technical field of medicine analysis.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing fingerprints of Danshen medicinal materials with high performance liquid chromatography (HPLC), and the standard fingerprints of medicinal materials Danshen obtained by the method. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Salvia miltiorrhiza is also known as red ginseng, purple salvia miltiorrhiza, red root and so on. The main effects are promoting blood circulation and regulating menstruation, dispelling blood stasis and relieving pain, cooling blood and eliminating carbuncle, clearing away heart-fire and restlessness, nourishing blood and calming nerves. Salvia root mainly contains fat-soluble diterpenes and water-soluble phenolic acids, and also contains flavonoids, triterpenes, sterols and other components. It is mainly used clinically for the treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 秦绪江单钰毓赛春梅刘文亮曲林凌雪宇李殿明
Owner 哈药集团中药二厂
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