The invention provides a method for measuring three active ingredients in Zhikeningsu capsules at the same time. The method comprises the specific steps that firstly,
chlorogenic acid,
hesperidin and
hyperoside are precisely weighed, then placed in 5 mL volumetric flasks respectively and dissolved with methyl
alcohol respectively, the volume is set to the calibration line, standard substance solutions are prepared respectively, then the standard substance solutions are transferred respectively to be mixed, and a mixed reference substance solution is prepared; secondly, the Zhikeningsu capsules are obtained, the
capsule shells are removed, precise weighing is conducted, Soxhlet extraction is conducted by using methyl
alcohol as a
solvent for 5 h, the extracting solution is cooled to the
room temperature, then transferred to a 100 mL
volumetric flask for
volume setting with methyl
alcohol and used as a
test sample solution, and the
test sample solution is used for detection; thirdly, separation detection is conducted, wherein the TC-C18
chromatographic column is used,
acetonitrile as a mobile phase A and a 0.2% glacial
acetic acid water solution as a mobile phase B are used for
gradient elution, the flow velocity is 1.0 mL.min<-1>, the VWD is adopted to detect the wave length, the
wavelength is 327 nm within 0-10 min, the
wavelength is 280 nm within 10-48 min, the
column temperature is 25 DEG C, and the sample size is 10 microliter. The method is simple in operation, reliable in result and capable of providing a reference basis for the
quality control over the Zhikeningsu capsules.