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Method for determining content of multi-component chemical components in Chinese pulsatilla root decoction

A technology of chemical composition and Pulsatilla decoction, applied in the field of determination of multi-component chemical components of Pulsatilla decoction, can solve the problems of not being widely used, and achieve the effects of high application value, perfect quality control research, and stable manuscript

Active Publication Date: 2022-01-04
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the clinical application of Pulsatilla Decoction is not extensive, and only the active ingredient of Pulsatilla Pulsatillae—Pulsatilla saponin is actually used clinically, which has anti-inflammatory effect

Method used

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  • Method for determining content of multi-component chemical components in Chinese pulsatilla root decoction
  • Method for determining content of multi-component chemical components in Chinese pulsatilla root decoction
  • Method for determining content of multi-component chemical components in Chinese pulsatilla root decoction

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Embodiment 1

[0064] Embodiment 1 of the present invention discloses a method for determining the content of multi-component chemical components of Pulsatillae decoction, comprising the following steps:

[0065] (1) Preparation of reference substance solution

[0066] Precisely weigh Pulsatilla saponin B4, Pulsatillae saponin B5 (Hderberry C), Black Sea Hedera saponin A1, Pulsatillae D, Pulsatillae A3, α-hederin, auretin, arculetin, aeretin B, corkine , palmatine hydrochloride, berberine hydrochloride, berberine hydrochloride, add methanol to dissolve, obtain the mass concentration of Pulsatilla saponin B4 in the reference substance stock solution is 28.2ng / mL, the mass concentration of Pulsatillae saponin B5 is 19.8ng / mL, black sea The mass concentration of hedera saponin A1 is 33ng / mL, the mass concentration of Pulsatilla saponin D is 23ng / mL, the mass concentration of Pulsatilla saponin A3 is 20.8ng / mL, and the mass concentration of α-hedera saponin is 23.4ng / mL, The mass concentration ...

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Abstract

The invention discloses a method for determining the content of multi-component chemical components in Chinese pulsatilla root decoction. The method specifically comprises the following steps: respectively establishing standard curves of anemosides B4, B5, D and A3, hederasaponin A1, alpha-hederasaponin, aesculin, aesculetin, fraxetin, phellodendrine, palmatine hydrochloride, coptisine hydrochloride and berberine hydrochloride by utilizing an HPLC-MSMS (High Performance Liquid Chromatography-Mass Spectrometry Mass Spectrometry) analysis method; measuring a Chinese pulsatilla root decoction sample extracted by a decoction method, making a standard curve, and measuring the content of the 13 chemical components. A scientific basis is provided for quality control of the Chinese pulsatilla root decoction and related preparations thereof.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for determining the content of multi-component chemical components of Pulsatilla Decoction. Background technique [0002] The chemical components in the prescription of traditional Chinese medicine are complex and there are many targets. The single decoction or joint decoction of the prescribed medicine may change its chemical composition, and the sum of various chemical components is the material basis of its therapeutic effect. Therefore, in order to study the mechanism of action of traditional Chinese medicine prescriptions and clarify the material basis of their efficacy, it is necessary to study their chemical components. [0003] Pulsatilla decoction comes from Zhang Zhongjing's "Treatise on Febrile Diseases". The prescription includes 4 traditional Chinese medicines, among which the monarch medicine is Pulsatilla, the minister medicine is Cop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/04G01N30/06G01N30/02G01N2030/042Y02A50/30
Inventor 刘振杰谭小青许琼明唐红珍高红伟覃喜军孙雪萍
Owner GUANGXI UNIV OF CHINESE MEDICINE
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