Method for detecting HPLC fingerprint spectrum of wine steamed coptidis rhizoma

A detection method and fingerprint technology, which is applied in the field of HPLC fingerprint detection of wine steamed Coptis chinensis, can solve the problems of lack of specific research on wine steamed Coptis chinensis, and achieve the effects of good consistency, simple and fast preparation, and strong regularity

Active Publication Date: 2019-08-16
雅安迅康药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control of wine-steamed Coptis chinensis is based on the basis of Coptis chinensis, and there is a lack of specific research on wine-steamed Coptis chinensis
In addition, there is no report on the simultaneous determination of Grandisine, African tetrandrine, epiberberine, jatrorrhizine hydrochloride, coptisine hydrochloride, berbererythrine, palmatine hydrochloride, and berberine hydrochloride in wine steamed Coptis chinensis

Method used

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  • Method for detecting HPLC fingerprint spectrum of wine steamed coptidis rhizoma
  • Method for detecting HPLC fingerprint spectrum of wine steamed coptidis rhizoma
  • Method for detecting HPLC fingerprint spectrum of wine steamed coptidis rhizoma

Examples

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Effect test

Embodiment 1

[0046] Embodiment 1 The fingerprint detection method of steamed Coptis chinensis in wine of the present invention

[0047] 1) Preparation of reference solution:

[0048] Accurately weigh the reference substances Grandicin, Tetradine, epiberberine, jatrorrhizine hydrochloride, coptisine hydrochloride, berbererythrine, palmatine hydrochloride, and berberine hydrochloride, and place them in a 25mL volumetric flask. Dissolved in methanol to obtain the concentrations of Grandisine, Tetradine, Epiberberine, Jatrorrhizine Hydrochloride, Coptisine Hydrochloride, Berbererythrine, Palmatine Hydrochloride, and Berberine Hydrochloride were 1.36, 7.12, and 0.643 , 4.176, 18.33, 0.515, 15.708 and 58.32μg / ml mixed reference solution;

[0049] 2) Preparation of the test solution:

[0050] Accurately weigh 0.2g of the powder of Huanglian steamed with wine (through the No. 2 sieve), put it in a stoppered Erlenmeyer flask, add 50ml of methanol-hydrochloric acid (100:1) mixed solution precisely, ...

Embodiment 2

[0061] Example 2 Detection of component content in wine-steamed Coptis chinensis of the present invention

[0062] 1) Preparation of reference solution:

[0063] Accurately weigh the reference substances Grandicin, Tetradine, epiberberine, jatrorrhizine hydrochloride, coptisine hydrochloride, berbererythrine, palmatine hydrochloride, and berberine hydrochloride, and place them in a 25mL volumetric flask. Dissolved in methanol to obtain the concentrations of Grandisine, Tetradine, Epiberberine, Jatrorrhizine Hydrochloride, Coptisine Hydrochloride, Berbererythrine, Palmatine Hydrochloride, and Berberine Hydrochloride were 1.36, 7.12, and 0.643 , 4.176, 18.33, 0.515, 15.708 and 58.32μg / ml mixed reference solution;

[0064] 2) Preparation of the test solution:

[0065] Accurately weigh 0.2g of the powder of Huanglian steamed with wine (through the No. 2 sieve), put it in a stoppered Erlenmeyer flask, add 50ml of methanol-hydrochloric acid (100:1) mixed solution precisely, seal i...

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Abstract

The invention provides an HPLC fingerprint spectrum of wine steamed coptidis rhizoma. The HPLC fingerprint spectrum is detected by adopting HPLC. A detection method comprises the steps of 1) preparinga reference solution; 2) preparing a test solution; and 3) sucking the reference solution and the test solution to be injected into a liquid chromatograph, and recording a chromatogram. According tothe detection method for the HPLC fingerprint spectrum of the wine steamed coptidis rhizoma, the established fingerprint spectrum has strong specificity and is strong in characteristic peak regularityand good in consistency; the detection method can be used as a quality evaluation method of the wine steamed coptidis rhizome, and also can be used for measuring the content of groenlandicine, columbamine, epiberberine, jatrorrhizine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, berberine hydrochloride and/or berberrubine in the wine steamed coptidis rhizome; and the wine steamed coptidis rhizoma can be effectively identified.

Description

technical field [0001] The invention specifically relates to an HPLC fingerprint detection method of wine-steamed Coptidis rhizoma. Background technique [0002] Traditional Chinese medicine (TCM) is a medicine produced under the guidance of TCM theory, which is a collection of many chemical substances, and is effective in one or more of these components. Therefore, it is difficult to truly reflect the pleiotropic effects and integrity of traditional Chinese medicines, and it is difficult to accurately reflect the internal comprehensive quality of traditional Chinese medicines with the existing quality evaluation methods. Therefore, the quality control model of simultaneous determination of multiple components has become the key to the quality control of traditional Chinese medicine in the future. Quantitative analysis of multi-indicator components is the most direct method for quality control of traditional Chinese medicine, which can reveal the change of chemical componen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06Y02A50/30
Inventor 张海岳顺疆曹勇纪奇森王瑾曾海蓉
Owner 雅安迅康药业有限公司
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