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40results about How to "Easy and quick to prepare" patented technology

Method for rapid aided detection of micro-plastics in water environment samples by using Nile red dyeing

A method for rapid aided detection of micro-plastics in water environment samples by using Nile red dyeing belongs to the field of environmental pollution control. The method comprises the following steps: firstly preparing a Nile red dye solution with Nile red and an acetone solution; preparing an ink-dyed organic film; next, before the dyeing, adding the working solution of the Nile red dye solution filtered by the organic film to the water sample to dye the water into a light blue color; filtering the dyed water sample by the organic film dyed with ink; finally, placing the filtered organicfilm in a culture dish, and uniformly coating glue on the filter to fix the substance on the filter for subsequent analysis and use. The method utilizes micro-plastics on a hydrophobic surface to adsorb Nile red dye solution, and observes the fluorescent substance on the filter by a fluorescence microscope at a wavelength of 450-490 nm to perform Raman detection. The method provided by the invention is simple and quick to prepare the dyeing agent, good in adsorption effect, and simple in dyeing method, and is not only short in time consumption, low in cost, but also can eliminate interferenceof some non-plastic materials, compared with the direct visual inspection method.
Owner:DALIAN UNIV OF TECH

Method for characterizing damage effect of toxin to living cells by SERS spectroscopy

The invention provides a method for characterizing the damage effect of toxin to living cells by SERS (surface enhanced Raman scattering) spectroscopy. Application of SERS spectroscopy to living cellcharacterization not only expands the new application of SERS spectroscopy, but also has potential application prospects in study of living cell damage. A TAT functionalized SERS probe is employed asthe Raman enhancement substrate, and enters living cells in a transmembrane way through receptor dependence mediated endocytosis, thus enhancing the Raman signal of cells themselves. The average SERSspectrum change of the cells before and after the action of toxin is detected to realize characterization of cell damage, also differential spectrum analysis and PCA are utilized to contrast the difference of cells on which the toxin acts for different periods of time, thus identifying and differentiating cells under different states. In order to further verify the cell apoptosis level, the Annexin V-FITC/PI double-staining reagent and flow cytometry are utilized to quantify the apoptosis situation. The method provided by the invention can achieve real-time nondestructive monitoring of the damage effect of toxin to living cells, and is simple and practicable, convenient and efficient.
Owner:JIANGNAN UNIV

Metal ceramic composite riser tube and preparation method thereof

The invention provides a metal ceramic composite riser tube. The metal ceramic composite riser tube comprises a ceramic riser tube body and a metal flange annularly sleeving the ceramic riser tube body, a bonding layer is arranged between the ceramic riser tube body and the metal flange, a boss is annularly arranged on the upper side surface of the ceramic riser tube body, the metal flange comprises an upper metal flange body and a lower metal flange body which are fixedly connected through bolts, an elastic sealing gasket is arranged between the upper metal flange body and the lower metal flange body, a groove is formed in the upper end of the inner surface of the lower metal flange body, the groove is connected with the boss in a matched mode, and a reverse airflow channel is formed in the joint of the upper surface of the boss and the outer surface of the ceramic riser tube body. The invention further provides a preparation method of the metal ceramic composite riser tube. Through arrangement of the bonding layer, the matched connection of the boss and the groove and the fixed connection of the bolts, the connection tightness and the air tightness between the ceramic riser tubebody and the metal flange are improved, and the problem that molten aluminum increases iron and causes pollution due to the fact that the riser tube is easy to corrode at a high temperature is solved;and the preparation process is simple and rapid.
Owner:XIAN CHAOMA SCI TECH

Method for rapidly determining citric soluble phosphorus in feed raw material calcium hydrogen phosphate

The invention relates to a method for rapidly determining citric soluble phosphorus in a feed raw material, namely calcium hydrogen phosphate. The method comprises the following steps: (1) mixing a calcium hydrogen phosphate sample with a neutral ammonium citrate solution, maintaining the temperature at 63-67 for 0.5-1.5 hours, cooling to the room temperature, fixing the volume with water, uniformly shaking, and filtering, so as to obtain filtrate; and (2) carrying out determination by virtue of a colorimetric method, drawing a phosphorus standard working curve, acquiring the phosphorus content by virtue of the working curve, and calculating, so as to obtain the content of citric soluble phosphorus in the calcium hydrogen phosphate sample. Compared with the prior art, the method has the advantages that the safe and precise determination of citric soluble phosphorus in the feed raw material, namely calcium hydrogen phosphate can be realized within a short time, the operation is simple and convenient, the consumed time is short, the detection efficiency is high, a detection result is relatively stable, the repeatability is good, the errors are small, reference data can be provided for the purchasing and acceptance check of the feed raw material, and the quality safety of a feed is guaranteed.
Owner:ZHANGZHOU AONONG ANIMAL HUSBANDRY TECH +3
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