The invention belongs to the technical field of
spectral analysis and chemical quantitative detection, and particularly relates to a reverse
solvent spectral stripping quantitative
analysis method, which comprises the following steps of: acquiring a Raman spectrum by selecting reference solutions with different concentrations, performing
spectral subtraction by taking a high-concentration reference spectrum as a reference spectrum and a low-concentration spectrum as a to-be-processed spectrum, and eliminating a
solvent background, so as to obtain a Raman spectrum; the method comprises the following steps: obtaining a difference spectrum only containing solute characteristic peaks, carrying out
curve fitting on the difference spectrum, extracting a solute Raman characteristic
peak intensity value, establishing a
linear relation model of concentration and characteristic
peak intensity, taking a spectrum of a solution to be detected as a spectrum to be processed, and subtracting the spectrum from a high-concentration reference spectrum to obtain a reverse difference spectrum; and calculating the solute concentration in the solution to be detected according to the established model. The problems of poor detection precision, high
detection limit and the like of the low-concentration solute in the prior art are solved, thorough deduction and
signal enhancement of a
solvent background can be realized under the condition of not depending on pure solvent reference, and high-precision quantitative analysis of the low-concentration solute is realized.