The invention discloses a non-destructive inspection method of total amount of meat bacteria, comprising the following steps: S1, obtaining an original reflection spectrum image Rs of a sample to be detected and storing the image; S2, detecting the TVC of the sample as standard reference data with a standard plate colony counting method according to National Standard; S3, obtaining a relative reflection spectrum image R of the sample according to the FORMULA that R=(Rs-Rd)/(Rr-Rd), wherein Rd is a black image when an image obtaining system works with dark current, and Rr is a reflection spectrum image of a standard reference whiteboard; S4, correcting the image with an SNV method; S5, choosing a second-order differential spectrum for the image R after being corrected, and selecting the best wavelength representing the TVC of the sample to be detected with a step-by-step regression method; S6, extracting the data and TVC detection result of the second-order differential spectrum relative to the best wavelength, and dividing the data and TVC detection result into two sets respectively as correction set data and validation set data; S7, establishing an LS-SVM data model, and detecting the value of the TVC of the sample to be detected. The method is human-oriented and advanced with quick and pollution-free detection process so that our country is in line with developed countries in the field of food quality and safety.