The invention relates to an upstream channel 
estimation method and an upstream channel 
estimation system suitable for an LTE (Long Term Evolution)-Advanced 
system. The upstream channel 
estimation method comprises the steps of extracting 
pilot frequency 
subcarrier information on a receiving antenna, and carrying out conjugate multiplying on the 
pilot frequency 
subcarrier information and a local 
pilot frequency of a first layer to obtain a 
frequency domain channel coefficient of all transport 
layers subjected to 
aliasing; according to the length of a 
subcarrier, adding a virtual subcarrier with a corresponding length at the 
tail of a 
primary channel coefficient; carrying out IDFT (
Inverse Discrete Fourier Transform) calculation, and transforming to a 
time domain; adding a window in the 
time domain, because 
dislocation of a 
time domain impulse response of each 
transport layer occurs on the time domain, separating time domain impulse responses of different transport 
layers, and carrying out time domain 
cyclic shift to obtain a time domain 
impulse response corresponding to each 
transport layer; and carrying out DFT (Discrete Fourier Transformation) calculation on the time domain 
impulse response of each 
transport layer, transforming to the 
frequency domain, and removing the virtual subcarrier at the 
tail to obtain a 
frequency domain equivalent channel estimation matrix of each transport layer corresponding to the receiving antenna. With the adoption of the upstream channel estimation method and the upstream channel estimation 
system, the channel estimation problem after 
MIMO (
Multiple Input Multiple Output) is introduced into the LTE-A upstream can be effectively solved, and the channel estimation accuracy is also improved; and the method is also suitable for an LTE system.