The invention discloses an MIMO-OFDM channel estimation method considering same frequency interference. The method comprises the following steps that 1), an observation sample of MIMO-OFDM pilot signals is utilized, an estimation criterion of the least square method is based, and then initial estimation of a channel impulse response is obtained; 2), the initial estimation of the channel impulse response is utilized, a residual error estimation method is based, and then an initial estimation value of an interference covariance matrix is obtained; 3), the initial estimation value of the interference covariance matrix is utilized, Cholesky decomposition and time domain 'low-pass filtering' are based, and then an improved estimation value of the interference covariance matrix is obtained; 4), the improved estimation value of the interference covariance matrix is utilized, a maximum likelihood estimation method is based, and then an improved estimation value of the channel impulse response and an improved estimation value of the channel frequency response are obtained. The MIMO-OFDM channel estimation method considering the same frequency interference is low in computation complexity and good in estimation error performance, and the performance gain is larger as the number of antennas is increased.