Total alkaloid of stephania delavayi and preparation and application thereof
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A technology of total alkaloids and gold not changing, applied in the field of medicine, can solve the problems such as pharmacological effects and clinical application of total alkaloids without gold changing, and achieve the effect of reducing the incidence of dysmenorrhea and reducing the contraction amplitude.
Active Publication Date: 2008-08-06
GUANGZHOU BOJI MEDICINE SERVICES
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Problems solved by technology
[0004] By analyzing the chemical components of Jinbuhuan medicinal material and the pharmacological effects of individual components, the inventors consulted domestic and foreign literature, and found no reports on the pharmacological effects and clinical application of Jinbuhuan total alkaloids.
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Embodiment 1
[0032] Take 1.0Kg of the coarse powder of Jinbuchang medicinal material, add 12 times the amount of 75% ethanol to percolate at a speed of 30ml / min / Kg medicinal material, and collect the percolation liquid. Ethanol was recovered under reduced pressure, concentrated into a thick paste (relative density 1.30, 50°C), dissolved in 5% sulfuric acid to 1000ml, filtered, adsorbed by a D101 macroporous resin column, washed with water until neutral, and then eluted with 95% ethanol. The eluate was collected, ethanol was recovered under reduced pressure, and vacuum-dried to obtain 55 g of total alkaloids of Jinbuhuan, which contained 75.8% of total alkaloids in terms of tetrahydropalmatine.
Embodiment 2
[0034] Take 1Kg of the coarse powder of the medicinal material of Jinbuchang, add 16 times the amount of 95% ethanol to percolate at a rate of 20ml / min / Kg of the medicinal material, and collect the percolation liquid. Recover ethanol under reduced pressure, concentrate to a relative density of 1.28 (50°C), dissolve with 5% sulfuric acid solution, filter, adjust the pH value of the filtrate to 9-11 with ammonia water, let it stand for 24 hours, filter, take the filter residue, and wash with water until Neutral, and then dissolved with 12 times the weight of 95% ethanol, recovered the ethanol under reduced pressure, and dried in vacuo to obtain 48 g of total alkaloids of Jin Bu Huan, containing 68.2% of total alkaloids in terms of tetrahydropalmatine.
Embodiment 3
[0036] Take 1Kg of Jinbuhuan medicinal material coarse powder, add 10 times the amount of 80% ethanol to reflux twice, filter, combine the ethanol solution, recover ethanol under reduced pressure, concentrate to a thick paste (relative density 1.30, 50°C), dissolve with 0.5% sulfuric acid 8000ml , filtered, adsorbed on a strong acidic cation exchange resin column, washed with water to remove impurities, took out the resin, added 2 times the amount of 5% ammonia water and ethanol to reflux extraction three times, each time for 1.5 hours, the extract was concentrated under reduced pressure, vacuum dried, and the total biological Alkali 40g, containing total alkaloids is 86.1% in terms of tetrahydropalmatine.
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Abstract
The invention discloses a total alkaloid of Stephania delavayi Diels, the content of which is measured by tetrahydropalmatine. Tetrahydropalmatine occupies 55-95 percent of the total alkaloid extract of Stephania delavayi Diels, wherein the weight percentage of tetrahydropalmatine contained is no less than 2 percent, while the weight percentage of palmatine hydrochloride contained is no less than 0.8 percent. The invention also discloses a preparation method of the total alkaloid of Stephania delavayi Diels, which relates to the steps as follows: adding the Stephania delavayi Diels meal into ethanol for diacolation or reflux to acquire alcohol extract, decompressing and recycling the ethanol and concentrating into a condensed ointment for dissolving and filtrating with acid to acquire a filtrate, passing the filtrate through a macroporous resin column or a strong acid cation exchange resin column for adsorption, washing with water to remove the impurities and extracting with a eluent, collecting the eluent and decompressing and recycling the extract and then concentrating and drying, thus acquiring the total alkaloid of Stephania delavayi Diels; the steps can also be decompressing a percolating liquid to recycle the ethanol, concentrating, dissolving with acid and then filtrating, adjusting the pH value of the filtrate with weak base to produce deposition, filtrating the deposition to acquire a filter residue, adding ethanol into the filter residue to resolve and decompress, recycle the ethanol and then drying, thus acquiring the total alkaloid. The results of a pharmacological study on the acquired total alkaloid show that the total alkaloid of Stephania delavayi Diels has good effect on spasmolysis, pain relieving, antiphlogosis and antibiosis, blood activating and so on, and can combine with the acceptable components in pharmacy to prepare various sustained release or controlled release formulation or other formulations.
Description
technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a medicinal material extract of Jinbuhuan-Jinbuhuan total alkaloids, and meanwhile, the invention also relates to a preparation method and application of Jinbuhuan total alkaloids. Background technique [0002] Jin Buhuan is the dry root tuber of the plants of the family Fangjiceae: Guangxi Dirong Stephania kwangsiensis H.S.Lo, Xiaohua Dirong Stephania micrantha H.S.Lo et M.Yang or Guangnan Dirong Stephaniakuinanensis H.S.Lo et M.Yang. Function Clearing heat and detoxifying, dissipating stasis and reducing swelling, invigorating the stomach and relieving pain; used for stomach and duodenal ulcer pain, upper respiratory tract infection, acute gastroenteritis, bacillary dysentery, toothache, neuralgia, carbuncle sore, swelling and pain from bruises . [0003] Studies have shown that Jinbuhuan medicinal materials mainly contain alkaloids, mainly tetrahydropalmaine, pal...
Claims
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