Disclosed are juvenile abalone fodder and a preparation method thereof. The juvenile abalone fodder comprises white fishmeal, sargassum fusiforme powder, kelp powder, gracilaria lemaneiformis powder, undaria pinnatifida powder, sargassum thunbergii powder, cylindrotheca powder, haematococcus pluvialis powder, diatom powder, schizochytrium powder, sodium alginate, fucoidin, mannitol, schizochytrium oil, chlorella oil, chaetoceros mulleri oil, zinc sulfate medical stone powder, VB2, inositol, vitamin-C phosphate magnesium, VA, VD3, calcium pantothenate, and zeolite powder. The method includes uniformly mixing and sieving all the powder, adding dissolved sodium alginate to mix to obtain bio-material, feeding the bio-material into a double-axis differential tempering machine to obtain ripe materials, expanding and shaping into wet flake fodder, and drying the flake fodder, pouring schizochytrium oil and then spraying and filming; drying in vacuum to obtain flake fodder. The fodder can meet the nutrition needs of juvenile abalone, is beneficial to invigorating juvenile abalone, promoting growth and increasing survival rate.