This invention relates to the fields of
biotechnology and
pharmacogenomics detection, and discloses a kit and application for
gene detection of drugs used in general
anesthesia. The kit contains a primer set for detecting genetic polymorphic loci related to commonly used
perioperative anesthetic,
analgesic, and
muscle relaxant drugs. The primer set includes
multiplex PCR amplification primers and single-base extension primers. The kit also includes
multiplex PCR amplification reaction solution, SAP reaction solution, SAP
enzyme, extension buffer, single-base extension termination mixture, extension reaction
enzyme, desalting purification resin, standards, calibrators, deionized water, and
instructions for use. This invention uses matrix-assisted
laser desorption /
ionization time-of-flight
mass spectrometry (MALDI-TOF) to
genotype SNP loci, obtaining
genotype results for 103 genetic polymorphic loci in a
single test. This allows for the prediction of individual differences in
drug metabolism,
drug efficacy, and adverse reaction risk, providing a basis for personalized medication in general
anesthesia.