The invention discloses a preparation method of soluble ampicillin powder for exported poultry. Soluble ampicillin powder is prepared from 10% of ampicillin, deoxyglucose, an anti-inflammatory synergist and nimesulide, wherein ampicillin is semi-synthesized broad-spectrum penicillin, deoxyglucose is a broad-spectrum antiviral agent, the anti-inflammatory synergist can improve the non-specific anti-inflammation effect of a medicine, nimesulide is a novel pain-relieving, anti-inflammatory and antipyretic medicine, and the soluble ampicillin powder is prepared from 10% of ampicillin, deoxyglucose, an anti-inflammatory synergist and nimesulide through mixing. The soluble ampicillin powder for exported poultry has a special effect on low immunity, high fever and inflammation caused by various viruses and toxins, the design is optimized, and the requirements of design are met.