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Preparation method of spirulina extract

A technology of spirulina extraction and spirulina, which is applied in the field of biological extraction of the main chemical components of spirulina, can solve the problems that the application value is not fully reflected, cannot destroy spirulina, and is difficult to release proteins, etc., achieving good application prospects and promotion value , easy absorption and utilization, and the effect of increasing the content of oligopeptides

Inactive Publication Date: 2012-06-27
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, commercially available spirulina products are mainly spirulina fine powder or tablets or capsules made by conventional preparation methods with spirulina fine powder, which has the following problems: one, spirulina has a heavier fishy smell, and now Some spirulina products fail to eliminate the fishy smell, causing olfactory stimulation and taste discomfort; second, the protein contained in spirulina mainly exists in the cells of spirulina, and simple processing methods cannot destroy the cell wall of spirulina, and it is difficult to release the protein contained in spirulina. Third, most of the proteins contained in spirulina are high proteins with a molecular weight of more than 50,000, which can only be absorbed and utilized after being digested into small molecule peptides by the human body, but the effective digestion and absorption rate of the human body is very low; in addition, When high protein intake is too much, there will be more side effects
Therefore, although the nutritional value of spirulina is very high, its real application value has not been fully reflected at present.

Method used

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  • Preparation method of spirulina extract
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  • Preparation method of spirulina extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of embodiment one spirulina extract

[0063] The preparation method of the present embodiment spirulina extract comprises the following steps:

[0064] a. Pre-fermentation treatment

[0065] Take food-grade spirulina fine powder (Shandong Wudi Foster Biological Engineering Co., Ltd.) with an average particle size of 150-250 μm, weigh it, and pass through a 60-mesh sieve to remove impurities and agglomerates. The obtained fine powder is placed in liquid nitrogen (temperature -123°C) for 4-5 times, each time for 2 minutes, so that the powder is fully frozen to the critical point of fragmentation (that is, the glass point); then the frozen powder is placed in a disc jet mill, and the high-speed impact of the airflow Movement, crush the frozen powder into superfine powder with an average particle size of 60-80 μm, dry and set aside;

[0066] Take food-grade stachyose (Xi'an Dapeng Biotechnology Co., Ltd.), and set aside;

[0067] Take 25 g of pig liver ...

Embodiment 2

[0074] The preparation of embodiment two spirulina extracts

[0075] The present embodiment spirulina extracting method comprises the following steps:

[0076] a. Pre-fermentation treatment

[0077] Take food-grade spirulina fine powder (Shandong Wudi Foster Biological Engineering Co., Ltd.) with an average particle size of 150-250 μm, weigh it, pass through a 60-mesh sieve, remove impurities and agglomerates, and place the obtained fine powder in a deep-low temperature refrigerator ( temperature -85°C) for 1 hour, so that the powder is fully frozen to the critical point of fragmentation (that is, the glass point); then the frozen powder is placed in a circulating tubular jet mill, and the frozen powder is crushed by the high-speed impact movement of the airflow Grinding to superfine powder with an average particle size of 60-80 μm, drying, and setting aside;

[0078] Take food-grade stachyose (Xi'an Dapeng Biotechnology Co., Ltd.), and set aside;

[0079] Take 100 g of f...

Embodiment 3

[0086] The preparation of embodiment triple spirulina extract

[0087] The preparation method of the present embodiment spirulina extract comprises the following steps:

[0088] a. Pre-fermentation treatment

[0089] Take food-grade spirulina fine powder (Shandong Wudi Foster Biological Engineering Co., Ltd.) with an average particle size of 150-250 μm, weigh it, pass through a 60-mesh sieve, remove impurities and agglomerates, and place the obtained fine powder in a deep-low temperature refrigerator ( temperature -50°C) for 3 hours, so that the powder is fully frozen to the critical point of fragmentation (that is, the glass point); then the frozen powder is placed in the jet mill, and the frozen powder is crushed by the high-speed impact movement of the air flow. Grinding to superfine powder with an average particle size of 60-80 μm, drying, and setting aside;

[0090] Take food-grade stachyose (Xi'an Dapeng Biotechnology Co., Ltd.), and set aside;

[0091] Take 25 g of...

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Abstract

The invention discloses an extraction method of a spirulina organism, which comprises the following three steps: a fermentation preprocessing step: super macro pulverizing spirulina fine powders, and adding a complex medium to carry out fermentation to prepare fermentation basic solution; a fermentation step: inoculating eosinophilic spirulina bifidobacterium with the fermentation basic solution, and carrying out fermentation to prepare fermentation liquor; and a fermentation postprocessing step: carrying out thallus removal and enzyme inactivation on the fermentation liquor, concentrating, and drying to prepare a spirulina main chemical componet extract. According to the invention, a process combining a super macro pulverization physical method and a bifidobacterium fermentation biological method is adopted for processing spirulina fine powder, so that the unpleasant fishy smell of the spirulina can be effective removed, and the taste of an end product is improved; an eucaryotic cell structure of the spirulina can be effectively damaged, and the contained protein is disassociated, so that the extraction rate of the protein is improved; the high protein can be completely resolved to translate into oligopeptides, and the content of the oligopeptides is improved, so that the end product is easy to absorb and utilize; and the method is simple and convenient to operate, has low cost and little environment pollution.

Description

technical field [0001] The invention relates to an extraction method of main chemical components in plants, in particular to a biological extraction method of main chemical components of spirulina. Background technique [0002] Spirulina (Spirulina) is a kind of multicellular filamentous microalgae, which is extremely rich in nutrients, with a protein content of 60-70%, and a reasonable protein composition. It is rich in eight kinds of amino acids that are necessary for the human body but cannot be synthesized by itself. The best natural protein food source found so far, in addition, spirulina is also rich in vitamins and minerals. A large number of studies have shown that spirulina has health effects in reducing blood fat, anti-cancer, anti-aging, anti-radiation, protecting the liver, improving the body's immunity and hematopoietic function, and regulating metabolic functions. At present, commercially available spirulina products are mainly spirulina fine powder or tablets...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/337A23L17/60
Inventor 吴力克毛青王宇明
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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