Preparation method for allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis

An early diagnosis and sensor technology, applied in the field of clinical medicine, can solve the problems that cannot be used in primary hospitals or large-scale censuses, time-consuming analysis, expensive, etc., and achieve the effects of being conducive to popularization, easy operation, and low price

Inactive Publication Date: 2016-01-06
SICHUAN UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a color-changing sensor for rapid identification and early diagnosis of IgA nephropathy, which aims to solve the current in vitro early diagnosis method for IgA nephropathy that requires training of professional operators, complicated equipment, time-consuming analysis, and high price , which cannot be used in primary hospitals or large-scale censuses

Method used

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  • Preparation method for allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis
  • Preparation method for allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis
  • Preparation method for allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis

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preparation example Construction

[0025] Such as figure 1 As shown, the method for preparing a color-changing sensor for rapid identification and early diagnosis of IgA nephropathy according to the embodiment of the present invention includes the following steps:

[0026] S101: obtaining a stable dispersion of diacetylene-like vesicles by thin film method or injection method;

[0027] S102: then adding the lectin dissolved in a buffer solution with a certain pH value to the diacetylene vesicle dispersion in a certain proportion;

[0028] S103: Finally, through physical adsorption or chemical coupling at a certain temperature, the lectin in the solution is combined or modified with diacetylene vesicles to obtain IgA with specific recognition function for GalNAC (N-acetylgalactosamine) Early recognition and diagnosis tool for kidney disease. Keep away from light.

[0029] In the color-changing sensor preparation method of the present invention, the diacetylene compound is 10,12-heptacosadiynoic acid (10,12-he...

Embodiment 1

[0045] Dissolve 10,12-pentacosadiynoic acid (10,12-pentacosadiynoic acid) in chloroform, use rotary evaporation to remove chloroform, add ultrapure water, and then ultrasonically hydrate to obtain non-polymerized butanediol prepared by thin film method Alkyne vesicle dispersion, and then irradiate the vesicle solution with ultraviolet light with a wavelength of 254nm to obtain a blue polymerized diacetylene vesicle dispersion. Dissolve the broad bean lectin (VVL) in a phosphate buffer solution with a pH of 7.4, add it to the dispersion of polymerized diacetylene vesicles, incubate at 25°C, and make the probe lectin in the solution by physical adsorption Coupled with diacetylene vesicles, the molar ratio of lectin to diacetylene vesicles is 1:1, and stored at room temperature in the dark.

[0046] At room temperature, add 0.5ml of the target detection sugar fragment GalNAC with a concentration of 0.01ug / ml to 1mL of the blue detection reagent, and it will change from blue to re...

Embodiment 2

[0048] Dissolve 10,12-nonacosadiynoic acid (10,12-nonacosadiynoic acid) in chloroform, remove chloroform by rotary evaporation, add ultrapure water, and then ultrasonically hydrate to obtain non-polymerized butanediol prepared by thin film method Alkyne vesicle dispersion, and then irradiate the vesicle solution with ultraviolet light with a wavelength of 254nm to obtain a blue polymerized diacetylene vesicle dispersion. Dissolve snail agglutinin (HAA) in a citrate buffer solution with a pH of 6, add it to the dispersion of polymerized diacetylene vesicles, incubate at 10°C, and agglomerate the probes in the solution by physical adsorption The lectin is coupled to diacetylene vesicles, the molar ratio of lectin to diacetylene vesicles is 1:5, and stored at room temperature in the dark.

[0049] At room temperature, 1ml of the target detection substance GalNAC with a concentration of 5ug / ml was added to 2ml of the blue detection reagent, and the color changed from blue to red w...

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Abstract

The invention discloses a preparation method for an allochroic sensor used for rapid identification and early diagnosis of IgA nephrosis. The preparation method includes obtaining stable butadiyne-type vesicle dispersoid by using a film method or an injection method; dissolving a probe agglutinin in a buffer having pH of 6-8, adding the agglutinin into the butadiyne-type vesicle dispersoid in mol ratio of 1:1 to 1:5; modifying the surface of butadiyne-type vesicle by the agglutinin in the solution in a physical absorption or chemical coupling manner at the temperature of 4-37 DEG C to obtain a tool having a specific recognition function for N-acetylgalactosamine in serum wherein N-acetylgalactosamine is the core fragment of the IgA1 marker having abnormal O-glycosylation. The preparation method is simple, rapid, accuracy and economy, and the price of the product is far lower than that of the like product. The preparation method for an allochroic sensor is used for popularizing the rapid identification, early diagnosis and general examination of IgA nephrosis.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, and in particular relates to a method for preparing a color-changing biosensor for rapid identification, early diagnosis and general survey of IgA nephropathy. Background technique [0002] IgA nephropathy is a glomerulonephritis pathologically characterized by IgA deposition in the mesangial region of the glomeruli, and is the most common primary glomerular disease in adolescents worldwide (especially in East Asia). IgA nephropathy is a progressive disease, and statistics show that only 5-30% of patients with abnormal urine tests can be completely relieved, and most of them develop chronically and progressively. 20-40% of diagnosed patients will enter end-stage renal disease (ESRD) within 5-25 years. Because IgA nephropathy has various clinical manifestations, complex pathological changes and different prognosis, most scholars believe that IgA nephropathy is not a single disease, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 蒋波孙鹏李霞
Owner SICHUAN UNIV
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