Kit for detecting Tn antigen on CA125 surface

A CA125 and kit technology, applied in the field of molecular biology, especially glycobiology, can solve the problems of antibody-lectin ELISA kits that have not yet been seen, and achieve the effects of high accuracy, time-saving detection, and easy access

Active Publication Date: 2015-03-11
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] Researchers in this field try to use the above-mentioned differences in glycosylation status to distinguish the elevated CA125 caused by benign or malignant diseases, so as to further improve the accuracy of clinical diagnosis, but so far, there is no antibody for the detection of Tn antigen on the surface of CA125- Report on Lectin ELISA Kit

Method used

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  • Kit for detecting Tn antigen on CA125 surface
  • Kit for detecting Tn antigen on CA125 surface
  • Kit for detecting Tn antigen on CA125 surface

Examples

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Embodiment 1

[0029] 1) Collect serum samples

[0030] Collect 91 gynecological cases with elevated serum CA125 levels, including 40 patients with malignant gynecological tumors and 51 patients with benign gynecological diseases; according to the conventional serum separation method, first draw 5ml of venous blood, and centrifuge at 2000g for 10 minutes in the coagulation tube after clotting. Aspirate the upper serum, freeze at -80°C, and thaw at 4°C before use.

[0031] 2) Derivatization of antibody sugar chains

[0032] (1) Coating: CA125 antibody (clone number: [X325]) is used as a coating solution (pH 9.6, 0.05MNa) 2 CO 3 -NaHCO 3 Solution) Dilute to 1μg / ml, add to the microtiter plate, 100μl / well, 4℃, overnight;

[0033] (2) Wash the plate: 0.1PBST, 200μl / well, wash the plate three times;

[0034] (3) Blocking: blocking solution (1*PBS containing 1% BSA), 200μl / well, 37°C, 1h;

[0035] (4) Oxidation: Oxidation solution (containing 20mM NaIO 4 0.1M sodium acetate solution, pH5.5), 200μl / well, 4℃,...

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Abstract

The invention belongs to the molecular biology field, and especially the glycobiology field, which provides a kit for detecting a Tn antigen on a CA125 surface. The invention relates to the kit for detecting an antibody-agglutinin ELISA of Tn antigen on the CA125 surface. The kit can capture the CA125 in serum through the CA125 antibody coated on a 96 orifice plate, the specificity is used for recognizing the agglutinin VVA of Tn antigen to detect the Tn antigen content on the CA125 surface, thereby whether the patient has malignant gynecologic tumor due to rising of CA125 or not can be determined. The time for kit detection is saved, a required serum sample is easy to be acquired, and the kit has high degree of accuracy used for distinguishing rising of benign CA125 and malignant CA125.

Description

Technical field [0001] The present invention belongs to the field of molecular biology, especially glycobiology, and relates to a kit for detecting the surface Tn antigen of CA125 (cancer antigen 125, cancer antigen 125). Specifically, it relates to an antibody-lectin ELISA kit for detecting Tn antigen on the surface of CA125. Background technique [0002] The prior art discloses that cancer antigen 125 is a highly glycosylated mucin, in which the sugar chain part accounts for about 20%-70% of the entire molecular mass. Studies have shown that about 80% of patients with epithelial ovarian cancer have elevated serum CA125 levels, and their levels can well reflect the body’s tumor burden. Therefore, cancer antigen 125 (CA125) has become the most commonly used in gynecology. Serum tumor markers and main monitoring and follow-up indicators after clinical ovarian cancer treatment. According to research, in addition to ovarian cancer, serum CA125 levels are also found to be abnormall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
Inventor 徐丛剑王宜生尧良清刘晓艳李小平孙立新曹锐高迎春张蓉金志军滕银成陆佳琦程明军张晓燕
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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