30 results about "Unicellular organism" patented technology

The invention discloses a device and a method for rapidly detecting surviving unicellular organisms in ship ballast water. The device comprises a PDMS micro-fluidic chip and glass substrate G, wherein the PDMS micro-fluidic chip comprises a liquid storage hole A, a liquid storage hole B, a liquid storage hole C, a liquid storage hole D, a liquid storage hole E, a main channel, a first focusing channel, a second focusing channel, a detection channel, a first sorting channel and a second sorting channel, wherein the liquid storage hole A, the liquid storage hole B, the liquid storage hole C, the liquid storage hole D and the liquid storage hole E are inserted into a platinum electrode. The device has the beneficial effects that (1) full-automatic detection, sorting, counting and size determination of the surviving unicellular organisms in the ship ballast water are realized on the micro-fluidic chip, and the device is small in size and is portably and rapidly used for on-site analysis; and (2) a differential micro-fluidic chip and a signal differential amplification mechanism are adopted, and the signal to noise ratio of a detection signal can be improved, so that the detection precision is improved.

The present invention is directed to a unicellular organism system, such as a yeast, for producing geranylgeranyl pyrophosphate and a diterpene in vivo. The yeast cell preferably comprises an inducible nucleic acid sequence encoding geranylgeranyl pyrophosphate synthase, an inducible nucleic acid sequence encoding a soluble form of HMG-CoA reductase, a nucleic acid sequence of an allele that confers an increase in sterol metabolic flux and, in the diterpene-producing cell, a diterpene synthase.

The present disclosure relates to methods of integrating recombinant polynucleotides into genomes of unicellular organisms. In particular, the present disclosure relates to the modified unicellular organisms that contain integrated recombinant polynucleotides in their genomes and methods for production of commodity chemicals by the use of such organisms.

The invention discloses a real-time monitoring system and method of physical characteristics of unicellular organisms. The system comprises an optical projector, a PC, a photo-induction electro-deposition chip, a three-dimensional mobile platform, a signal generator, an impedance analyzer, a temperature controller, a micro pump and a camera; the optical projector is connected with the PC and projects light patterns onto the photo-induction electro-deposition chip; the photo-induction electro-deposition chip is arranged on the three-dimensional mobile platform, and the three-dimensional mobileplatform can change the position of the chip; the signal generator is connected with the chip and provides AC voltage; the impedance analyzer is connected with the chip and measures the impedance; thechip is arranged in the temperature controller and controls the continuous culture temperature of cells; the micro pump is connected with a solution inlet of the chip and provides a cell culture medium; and a microscopic tube is arranged above the chip, and the camera is connected with the microscopic tube and acquires an image in real time. By adopting the apparatus and method of the invention,the electric characteristics of adherent single cells in a full life cycle can be monitored in real time, and the location and addressing growth of the single cells can be performed.

The invention discloses a method for feeding wild catfish and carp living in Yellow River in ecosystem environment. The method comprises the following steps: injecting Yellow River water into common earth, crushing planted fodder grass; adding a composite micro-ecological viable bacteria preparation into the crushed fodder grass; adopting a closed anaerobic fermentation process to prepare a yeast material; scattering the yeast material once every ten days so as to provide nutrient components required by the growth of beneficial alga and plankton unicellular organisms to feed and breed daphnia, algae and float grass, and to feed wild trash fish fries and river prawns; and using Yellow River circulating water and living food to feed the wild catfish and carp. The method uses natural ecological resources to develop natural Yellow River wild fishes for breeding, forms a complete a natural food chain, and maintains natural quality of Yellow River fishes. The method is applied to both coasts of Yellow River valley, utilizes natural resources to develop wild fish ecological feeding in large scale, and provides a novel approach for developing domestic food resources.

Mineral functional water useful for controlling unicellular organisms and/or viruses is provided. The mineral functional water satisfies all of requirements (i), and (iii) and shows excellent controlling effects upon unicellular organisms and/or viruses. (i) In a sample wherein 15 pst·wt. or more of the mineral functional water is fixed with respect to 100 pst·wt. of a ceramic carrier, the average emissivity to black body at wavelength of 5 to 7 micrometers and wavelength of 14 to 24 micrometers (measurement temperature: 25 Centigrade) is 90% or more, (ii) pH of the mineral functional water is 12 or higher, and (iii) controlling effects against at least one of unicellular organisms and viruses are manifested.

The invention discloses a method for extracting pure diatom shells from marine unicellular diatom. The method comprises the following steps: (1) preparing algae mud; (2) treating algae mud by an acid-heat method; and (3) washing and drying. Compared with the prior art, the method has the advantages that the operation process is simple and feasible; the completeness is good, and the diatom shell treated by using the mixed acid solution of sulfuric acid and nitric acid is complete in structure and clear in pore; the cleanliness is high, organic matter and harmful solvent residues do not exist on the surfaces of the diatom shells, and the purity is high; the invention provides a simple and efficient method for extracting and separating high-purity diatom shells from marine diatom and other unicellular organisms containing the diatom shells, and further research of a factory-like large-scale diatom shell extraction and separation process is facilitated.

The invention discloses an OsABCG48 gene and an application thereof in improving cadmium stress resistance of unicellular organisms and plants. Rice OsABCG48 protein has an amino acid sequence as shown in SEQ ID NO.1 or a sequence which is obtained by substitution, deletion and/or addition of one or more amino acids and/or terminal modification for the amino acid sequence as shown in SEQ ID NO.1 and has the effect of improving cadmium stress resistance of unicellular organisms and plants. The gene for encoding the rice OsABCG48 protein is shown as SEQ ID NO.2. The OsABCG48 gene is introduced into unicellular organisms and plants, so that the OsABCG48 gene can be over-expressed in the unicellular organisms and plants, thereby improving cadmium stress resistance. The OsABCG48 gene disclosedby the invention has extremely high development potential in cultivation of new cadmium-resistant unicellular biological strains and new cadmium-resistant crop strains.

A system and method for micromanipulating samples are described to perform automatic, reliable, and high-throughput sample microinjection of foreign genetic materials, proteins, and other molecules, as well as drawing genetic materials, proteins, and other molecules from the sample. The system and method overcome the problems inherent in traditional manual micromanipulation that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for adherent cell microinjection but can be readily extended to aspiration, isolation, and electrophysiological measurements of microorganisms, unicellular organisms, or cells.

The invention discloses a high efficient foliar fertilizer utilizing algae culture complexing and a preparation method thereof. The foliar fertilizer is prepared from the following raw materials: plant derived fresh feed, ethyl acetate, ethanol, diethyl aminoethyl hexanoate, chitin, green algae powder, straw ash, spirulina powder, sugar dregs, pig bone soup, ammonium molybdate, sodium selenite, soybean milk, potassium chloride, rice washing water, and a proper amount of water. Cheap organic substances are used to culture algae; algae is a unicellular organism and the ancestor of most plants in the earth and has the function of enriching trace elements; trace elements are added into the culture solution and enriched by the algae cells, the inorganic trace elements are converted into organic substances, finally the cultured algae cells are killed to expose complexed trace elements; the trace elements are used to prepare the foliar fertilizer, and the absorption efficiency of trace elements is improved.

Provided are novel compounds and uses thereof in preventing antifouling by unicellular organisms and in attracting cells from multicellular organisms.

A process to ferment lactose and molasses, to obtain unicellular biomass is disclosed. The described process allows obtaining a product of high protein value which will be in the range of 50 to 90% for its application in the food industry. The process uses as a means of propagation whey of cheese or molasses, for the use by Kluyveromyces marxianus to obtain unicellular biomass and subsequently unicellular protein.

The present invention is directed to nucleic acid sequences of Ginkgo biloba diterpene synthases, particularly of a levopimaradiene synthase. More specifically, the invention is directed to a cell of a unicellular organism, such as Saccharomyces cerevisiae or Escherichia coli, comprising levopimaradiene synthase for the metabolically engineered in vivo biosynthesis of a diterpene and a ginkgolide.

A wastewater treatment plant and related method comprise a treatment stage including a biological-process substage configured for growing unicellular organisms adapted to reduce contaminants in the wastewater which are dissolved, including at least one of organic matter and nitrogenous matter, by digestion thereof, and which are adapted to floc after digestion and a floc-removal substage downstream from the biological-process substage, relative to the flow of wastewater, and configured for substantially removing the unicellular organisms that have flocked. The treatment stage is configured to form majority and minority flows of treated wastewater, and the minority flow is configured to be recycled upstream of the biological-process treatment substage. The plant includes a heat transfer assembly configured for transferring heat from the majority flow of treated wastewater to the minority flow thereof to increase temperature of wastewater to be treated.

A comprehensive nutrition pet food that satisfies a nutritional food standard for a dog or a cat, the comprehensive nutrition pet including a unicellular organism ingredient containing astaxanthin and being free of meals, in which a content of the astaxanthin is 0.0012% to 0.0080% by mass, relative to a total amount of the comprehensive nutrition pet food, and an a* value of the comprehensive nutrition pet food in an L*a*b* color space is 8.0 to 20.