30 results about "Unicellular organism" patented technology

The present invention is directed to a unicellular organism system, such as a yeast, for producing geranylgeranyl pyrophosphate and a diterpene in vivo. The yeast cell preferably comprises an inducible nucleic acid sequence encoding geranylgeranyl pyrophosphate synthase, an inducible nucleic acid sequence encoding a soluble form of HMG-CoA reductase, a nucleic acid sequence of an allele that confers an increase in sterol metabolic flux and, in the diterpene-producing cell, a diterpene synthase.

The present disclosure relates to methods of integrating recombinant polynucleotides into genomes of unicellular organisms. In particular, the present disclosure relates to the modified unicellular organisms that contain integrated recombinant polynucleotides in their genomes and methods for production of commodity chemicals by the use of such organisms.

The present invention is directed to nucleic acid sequences of Ginkgo biloba diterpene synthases, particularly of a levopimaradiene synthase. More specifically, the invention is directed to a cell of a unicellular organism, such as Saccharomyces cerevisiae or Escherichia coli, comprising levopimaradiene synthase for the metabolically engineered in vivo biosynthesis of a diterpene and a ginkgolide.

A method of determining structural data of a prototype for a lightweight technical structure by selecting shell architectures of natural bio-mineralized unicellular organisms in accordance with an aspect set very closely adapted to the lightweight structure to be produced and by directly copying and thereafter scaling their structural data to the prototype before optimizing the Prototype by perfect adaptation to the lightweight structure to be produced.

Provided are novel compounds and uses thereof in preventing antifouling by unicellular organisms and in attracting cells from multicellular organisms.

Provided is a mineral functional water useful for controlling unicellular organisms or viruses. The mineral functional water satisfies all of requirements (i) through (iii) and manifests an excellent controlling effect on unicellular organisms and viruses. (i) In a sample wherein 15 parts by weight or more of the mineral functional water is fixed with respect to 100 parts by weight of a ceramic carrier, the average radiation ratio to black body at a wavelength of 5 to 7 [mu]m and wavelength of 14 to 24 [mu]m (measurement temperature: 25 DEG C) is 90% or higher, (ii) pH of the mineral functional water is 12 or higher, and (iii) a controlling effect is displayed against at least one of unicellular organisms and viruses.

The invention discloses a method for feeding wild catfish and carp living in Yellow River in ecosystem environment. The method comprises the following steps: injecting Yellow River water into common earth, crushing planted fodder grass; adding a composite micro-ecological viable bacteria preparation into the crushed fodder grass; adopting a closed anaerobic fermentation process to prepare a yeastmaterial; scattering the yeast material once every ten days so as to provide nutrient components required by the growth of beneficial alga and plankton unicellular organisms to feed and breed daphnia, algae and float grass, and to feed wild trash fish fries and river prawns; and using Yellow River circulating water and living food to feed the wild catfish and carp. The method uses natural ecological resources to develop natural Yellow River wild fishes for breeding, forms a complete a natural food chain, and maintains natural quality of Yellow River fishes. The method is applied to both coasts of Yellow River valley, utilizes natural resources to develop wild fish ecological feeding in large scale, and provides a novel approach for developing domestic food resources.

The invention discloses a real-time monitoring system and method of physical characteristics of unicellular organisms. The system comprises an optical projector, a PC, a photo-induction electro-deposition chip, a three-dimensional mobile platform, a signal generator, an impedance analyzer, a temperature controller, a micro pump and a camera; the optical projector is connected with the PC and projects light patterns onto the photo-induction electro-deposition chip; the photo-induction electro-deposition chip is arranged on the three-dimensional mobile platform, and the three-dimensional mobileplatform can change the position of the chip; the signal generator is connected with the chip and provides AC voltage; the impedance analyzer is connected with the chip and measures the impedance; thechip is arranged in the temperature controller and controls the continuous culture temperature of cells; the micro pump is connected with a solution inlet of the chip and provides a cell culture medium; and a microscopic tube is arranged above the chip, and the camera is connected with the microscopic tube and acquires an image in real time. By adopting the apparatus and method of the invention,the electric characteristics of adherent single cells in a full life cycle can be monitored in real time, and the location and addressing growth of the single cells can be performed.

A system and method for micromanipulating samples are described to perform automatic, reliable, and high-throughput sample microinjection of foreign genetic materials, proteins, and other molecules, as well as drawing genetic materials, proteins, and other molecules from the sample. The system and method overcome the problems inherent in traditional manual micromanipulation that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for adherent cell microinjection but can be readily extended to aspiration, isolation, and electrophysiological measurements of microorganisms, unicellular organisms, or cells.

The invention discloses an unicellular organism-based high-hydrophobicity micrometer powder material, and a preparation method thereof. According to the preparation method, unicellular organism cells are washed repeatedly, an activation agent is used for activation of the unicellular organism cells so as to obtain surface activated unicellular organism cells; at the same time, Fe3O4 nanoparticles are subjected to washing and modification so as to realize combination of the nanoparticles with agglutinin molecules, and obtain agglutinin molecule-modified nanoparticles; the agglutinin molecule-modified nanoparticles are combined with the surface-activated unicellular organism cells; construction of unicellular organism surface rough micro-nano structures is realized via immobilizing of the nanoparticles on the surface of the unicellular organism cells; and the surface of an obtained product is subjected to octadecylamine modification so as to reduce particle surface energy further, and obtain the unicellular organism-based high-hydrophobicity micrometer powder material.

The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacteria Defluviimonas sp. 20V17, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. The present nuclease possesses unusual properties and can be used as an instrument for introducing changes at strictly specified places in a genomic DNA sequence of single-celled and multi-celled organisms. The invention thus increases the universality of accessible CRISPR-Cas9 systems, which makes it possible to use Cas9 nuclease from various organisms to cut genomic or plasmid DNA in a large number of specific sites and under various conditions.