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Method for extracting pure diatom shells from marine single-cell diatom

A technology of pure diatom frustules and single cells, applied in the direction of silicon oxide, silicon dioxide, etc., can solve the problems of cumbersome operation process, mutual adhesion, broken shell, etc., and achieve simple operation process, simple operation process, and shell structure full effect

Inactive Publication Date: 2021-06-18
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this patent are: the operation process is relatively cumbersome, the purity of the diatom shells is not high, and they are adhered to each other; multiple centrifugation and calcinations lead to broken shells and a decrease in the number of silanol groups, which affects downstream applications
[0005] Chinese patent CN201780038312.3 discloses a diatom frustule extracted from benthic diatoms harvested by industrial biofilm treatment. The main disadvantage of diatom frustule extraction in this patent is that this method is more suitable for diatoms , for central diatoms, hydrogen peroxide is not enough to remove all organic matter, and continuous high-temperature calcination will destroy the surface groups of silica shells, affecting the application of diatom shells in the micro-nano field
[0006] The extraction method of siliceous shells (acid Combination of washing and roasting), the disadvantages of this method are: the operation process is relatively cumbersome (gradient ethanol dehydration + pickling + water washing + roasting), and long-term high-temperature calcination will cause the diatom shell to collapse, structural damage will also reduce its surface silanol content

Method used

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  • Method for extracting pure diatom shells from marine single-cell diatom
  • Method for extracting pure diatom shells from marine single-cell diatom
  • Method for extracting pure diatom shells from marine single-cell diatom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: purify Cyclotella shell

[0038] The above-mentioned cultivated Cyclotella cryptica ( Cyclotella cryptica ) into 100 mL centrifuge tubes, put them into a centrifuge for centrifugation after balancing, the centrifugal force is 2000 g, and the centrifugation time is 5 min. After the centrifugation is completed, discard the supernatant, combine the diatom mud in each tube, and wash with distilled water 3 times to remove inorganic salts and some impurities. Add 25 mL of concentrated sulfuric acid to the algae mud as a treatment solution to fully mix the algae cells with concentrated sulfuric acid, and place them in a water bath at 60°C for 45 min. The heated mixture was left at room temperature for 24 h; an equal volume of concentrated nitric acid was added to the mixture again, heated at 60°C until clear, and left at room temperature for 24 h. The treated diatom shells were collected by natural filtration through a sieve to avoid damage to the diatom shell...

Embodiment 2

[0044] Embodiment 2: Purification Thalassiosira wischii shell

[0045] The above-mentioned cultivated Thalassiosira wilsonii ( Thalassiosira weissflogii ) into 100 mL centrifuge tubes, and put them into a centrifuge for centrifugation with a centrifugal force of 2000 g and a centrifugation time of 5 minutes. After the centrifugation is completed, discard the supernatant, combine the algae mud in each tube, and wash 3 times with distilled water. The method for extracting pure diatom frustules is the same as in Example 1.

[0046] Stick the diatom frustule of Thalassiosira wilsonii on the double-sided tape, and spray gold on it, figure 1 It is a scanning electron microscope picture of Thalassiosira wilsonii. The results show that the shell structure of Thalassiosira wilsonii is complete, the pore size is transparent, and there is no organic matter residue ( figure 1 c).

[0047] figure 2 It is the result of energy spectrum analysis of Thalassiosira wilsonii, by figure...

Embodiment 3

[0050] Embodiment 3: purify ovale shell

[0051] The above-mentioned cultivated ovoid algae ( Cocconeiopsis orthoneoides ) with a 2500-mesh sieve to collect the algae cells and wash them three times with distilled water to remove inorganic salts and some impurities. The method for extracting ovale diatom frustules is the same as in Example 1. Through scanning electron microscopy (SEM) morphology analysis and energy dispersive X-ray spectrometer (EDXS) analysis of the extracted samples, the main components of diatom frustules were quantitatively analyzed with an elemental analyzer.

[0052] figure 1 It is a scanning electron microscope image of the ovoid algae shell, and the results show that the structure of the ovoid algae diatom shell obtained by the present invention is complete, the original shape and microstructure of the shell are retained, and there is no organic matter residue.

[0053] image 3 It is the infrared spectrogram of ovale algae. The results show that...

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Abstract

The invention discloses a method for extracting pure diatom shells from marine unicellular diatom. The method comprises the following steps: (1) preparing algae mud; (2) treating algae mud by an acid-heat method; and (3) washing and drying. Compared with the prior art, the method has the advantages that the operation process is simple and feasible; the completeness is good, and the diatom shell treated by using the mixed acid solution of sulfuric acid and nitric acid is complete in structure and clear in pore; the cleanliness is high, organic matter and harmful solvent residues do not exist on the surfaces of the diatom shells, and the purity is high; the invention provides a simple and efficient method for extracting and separating high-purity diatom shells from marine diatom and other unicellular organisms containing the diatom shells, and further research of a factory-like large-scale diatom shell extraction and separation process is facilitated.

Description

technical field [0001] The invention relates to the extraction of diatom frustules, in particular to a method for extracting complete and pure diatom frustules from single-cell diatoms. Background technique [0002] Diatoms are a group of photoautotrophic unicellular eukaryotic algae that are widely distributed in marine and freshwater ecosystems around the world. It is one of the most important primary productivity in the marine ecosystem, which can produce 4.5-5 billion tons of organic carbon every year, accounting for 40% of the primary productivity of the ocean and 20% of the global primary productivity. Diatoms have very strong adaptability to the environment, grow rapidly, and some species can be cultivated on a large scale, and can accumulate a large amount of biomass in a short period of time. Diatom frustule (diatom frustule) is the cell wall of diatoms. It is a solid shell formed by absorbing silicate in the environment and depositing it through the biomineralizat...

Claims

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Application Information

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IPC IPC(8): C01B33/18
CPCC01B33/18C01P2004/03C01P2002/82
Inventor 韩吉昌王路路张琳潘克厚
Owner OCEAN UNIV OF CHINA
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