Real-time monitoring system and method of physical characteristics of unicellular organisms

Abstract

The invention discloses a real-time monitoring system and method of physical characteristics of unicellular organisms. The system comprises an optical projector, a PC, a photo-induction electro-deposition chip, a three-dimensional mobile platform, a signal generator, an impedance analyzer, a temperature controller, a micro pump and a camera; the optical projector is connected with the PC and projects light patterns onto the photo-induction electro-deposition chip; the photo-induction electro-deposition chip is arranged on the three-dimensional mobile platform, and the three-dimensional mobileplatform can change the position of the chip; the signal generator is connected with the chip and provides AC voltage; the impedance analyzer is connected with the chip and measures the impedance; thechip is arranged in the temperature controller and controls the continuous culture temperature of cells; the micro pump is connected with a solution inlet of the chip and provides a cell culture medium; and a microscopic tube is arranged above the chip, and the camera is connected with the microscopic tube and acquires an image in real time. By adopting the apparatus and method of the invention,the electric characteristics of adherent single cells in a full life cycle can be monitored in real time, and the location and addressing growth of the single cells can be performed.

Description

Technical field [0001] The invention relates to the field of cell monitoring, in particular to a real-time monitoring system and method for the biophysical characteristics of single cells. Background technique [0002] The occurrence of diseases is closely related to cell behaviors such as cell proliferation, migration and canceration. Malignant tumor is a serious disease threatening human health. The main manifestation of its occurrence is the uncontrolled proliferation, invasion and metastasis of cancer cells. In traditional cell research, cell samples are usually regarded as uniform and stable, so that cell populations are mainly studied. However, in fact, cells are usually heterogeneous, and many diseases occur, and there are always very few cells in the early stage. Carcinogenesis occurs, and the signals of these cancerous cells are usually masked by the signals of a large number of normal cells. Therefore, the study of single cells is not only helpful for understanding th...

AI Technical Summary

Problems solved by technology

However, the above two methods can only detect suspension cells, and cannot monitor the electrical characteristics of the whole life cycle of single cells in the adherent state and the positioning and addressing growth of single cells.

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