A real-time monitoring system and method for single cell biophysical properties

Abstract

The invention discloses a real-time monitoring system and method of physical characteristics of unicellular organisms. The system comprises an optical projector, a PC, a photo-induction electro-deposition chip, a three-dimensional mobile platform, a signal generator, an impedance analyzer, a temperature controller, a micro pump and a camera; the optical projector is connected with the PC and projects light patterns onto the photo-induction electro-deposition chip; the photo-induction electro-deposition chip is arranged on the three-dimensional mobile platform, and the three-dimensional mobileplatform can change the position of the chip; the signal generator is connected with the chip and provides AC voltage; the impedance analyzer is connected with the chip and measures the impedance; thechip is arranged in the temperature controller and controls the continuous culture temperature of cells; the micro pump is connected with a solution inlet of the chip and provides a cell culture medium; and a microscopic tube is arranged above the chip, and the camera is connected with the microscopic tube and acquires an image in real time. By adopting the apparatus and method of the invention,the electric characteristics of adherent single cells in a full life cycle can be monitored in real time, and the location and addressing growth of the single cells can be performed.

Description

technical field [0001] The invention relates to the field of cell monitoring, in particular to a real-time monitoring system and method for single cell biophysical characteristics. Background technique [0002] The occurrence of diseases is closely related to cellular behaviors such as cell proliferation, migration and cancerization. Malignant tumor is a disease that seriously threatens human health. The main manifestation of its occurrence is the uncontrolled proliferation, invasion and metastasis of cancer cells. In traditional cell research, the cell sample is usually regarded as uniform and stable, so as to mainly study the cell population, but in fact cells are usually heterogeneous, and many diseases are always caused by a small number of local cells in the early stage. Cancerous changes occur, and the signals of these cancerous cells are often masked by the signals of a large number of normal cells. Therefore, the study of single cells not only helps to understand t...

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Problems solved by technology

However, the above two methods can only detect suspension cells, and cannot monitor the electrical characteristics of the whole life cycle of single cells in the adherent state and the positioning and addressing growth of single cells.

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