A real-time monitoring system and method for single cell biophysical properties

Abstract

The invention discloses a real-time monitoring system and method for single cell biophysical characteristics. The system includes: an optical projector, a PC, a light-induced electrodeposition chip, a three-dimensional mobile platform, a signal generator, an impedance analyzer, a temperature controller, a micro-pump and a camera; the optical projector is connected to the PC and the light pattern is Projected onto the light-induced electrodeposition chip; the light-induced electrodeposition chip is located on a three-dimensional moving platform, and the three-dimensional moving platform can change the position of the chip; the signal generator is connected to the chip to provide AC voltage; the impedance analyzer is connected to the chip to measure impedance; Place the chip in a temperature controller to control the temperature of continuous cell culture; the micropump is connected to the solution inlet of the chip to provide cell culture medium; the microtube is located above the chip, and the camera is connected to the microtube to collect images in real time. The device or method of the present invention can be used to conduct real-time monitoring of the electrical characteristics of the entire life cycle of adherent single cells and the positioning and addressable growth of single cells.

Description

technical field [0001] The invention relates to the field of cell monitoring, in particular to a real-time monitoring system and method for single cell biophysical characteristics. Background technique [0002] The occurrence of diseases is closely related to cellular behaviors such as cell proliferation, migration and cancerization. Malignant tumor is a disease that seriously threatens human health. The main manifestation of its occurrence is the uncontrolled proliferation, invasion and metastasis of cancer cells. In traditional cell research, the cell sample is usually regarded as uniform and stable, so as to mainly study the cell population, but in fact cells are usually heterogeneous, and many diseases are always caused by a small number of local cells in the early stage. Cancerous changes occur, and the signals of these cancerous cells are often masked by the signals of a large number of normal cells. Therefore, the study of single cells not only helps to understand t...

AI Technical Summary

Problems solved by technology

However, the above two methods can only detect suspension cells, and cannot monitor the electrical characteristics of the whole life cycle of single cells in the adherent state and the positioning and addressing growth of single cells.

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