Device and method for rapidly detecting surviving unicellular organisms in ship ballast water

Abstract

The invention discloses a device and a method for rapidly detecting surviving unicellular organisms in ship ballast water. The device comprises a PDMS micro-fluidic chip and glass substrate G, wherein the PDMS micro-fluidic chip comprises a liquid storage hole A, a liquid storage hole B, a liquid storage hole C, a liquid storage hole D, a liquid storage hole E, a main channel, a first focusing channel, a second focusing channel, a detection channel, a first sorting channel and a second sorting channel, wherein the liquid storage hole A, the liquid storage hole B, the liquid storage hole C, the liquid storage hole D and the liquid storage hole E are inserted into a platinum electrode. The device has the beneficial effects that (1) full-automatic detection, sorting, counting and size determination of the surviving unicellular organisms in the ship ballast water are realized on the micro-fluidic chip, and the device is small in size and is portably and rapidly used for on-site analysis; and (2) a differential micro-fluidic chip and a signal differential amplification mechanism are adopted, and the signal to noise ratio of a detection signal can be improved, so that the detection precision is improved.

Description

technical field [0001] The invention relates to a device and method for rapidly detecting surviving single-celled organisms in ship's ballast water, and in particular to a fully automatic detection, sorting, counting and counting of surviving single-celled organisms in ship's ballast water on a microfluidic chip Apparatus and method for dimension determination. Background technique [0002] The ship's ballast water is used to provide stability for the ship and adjust the draft and draft difference of the ship to meet the good maneuverability requirements of the ship. Loading part of the ballast water can ensure the safety of the ship when it is empty or partially empty and under severe sea conditions. [0003] Ship ballast water contains a large number of aquatic organisms, and the invasion of alien marine organisms caused by ship ballast water has been recognized by the Global Environment Fund (GEF) as one of the four major threats to the ocean. In order to better manage ...

AI Technical Summary

Problems solved by technology

However, these methods require the use of relatively mature equipment and instruments in the laboratory for analysis, which are bulky, time-consuming, and cannot be easily brought to the scene. Some detection methods cannot distinguish the life and size of microorganisms

Moreover, some testing instruments require specialized technicians to operate, so they are not suitable for

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