OsABCG48 gene and application thereof in improving cadmium stress resistance of unicellular organisms and plants
A single-cell, gene technology, applied in the field of genetic engineering, can solve the problem that the cost of engineering is not operable
Active Publication Date: 2021-01-05
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology
Among the existing methods, soil remediation is mainly used as a treatment method to deal with cadmium pollution in soil, but neither soil cleaning nor soil replacement can do anything for large-scale pollution, and its engineering volume and huge cost make it basically impossible Operability
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[0040] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.
[0041] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.
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The invention discloses an OsABCG48 gene and an application thereof in improving cadmium stress resistance of unicellular organisms and plants. Rice OsABCG48 protein has an amino acid sequence as shown in SEQ ID NO.1 or a sequence which is obtained by substitution, deletion and / or addition of one or more amino acids and / or terminal modification for the amino acid sequence as shown in SEQ ID NO.1 and has the effect of improving cadmium stress resistance of unicellular organisms and plants. The gene for encoding the rice OsABCG48 protein is shown as SEQ ID NO.2. The OsABCG48 gene is introduced into unicellular organisms and plants, so that the OsABCG48 gene can be over-expressed in the unicellular organisms and plants, thereby improving cadmium stress resistance. The OsABCG48 gene disclosedby the invention has extremely high development potential in cultivation of new cadmium-resistant unicellular biological strains and new cadmium-resistant crop strains.
Description
technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to the application of OsABCG48 gene in improving the cadmium-resistant ability of single-celled organisms and higher plants, including crops. Background technique [0002] Cadmium (Cd) is a toxic heavy metal, one of the most threatening pollutants to the environment and one of the most toxic elements to living cells. As a biologically non-essential metal, it can replace other essential metals. After entering the human body, it can cause ion homeostasis imbalance, protein dysfunction, DNA and plasma membrane damage, and the accumulation of reactive oxygen species. Cadmium is also a carcinogen, and accumulation of cadmium in the body can lead to weak bones and kidney failure. Cadmium is mainly dependent on food intake. Cadmium has a long biological half-life in the kidney (about 10-30 years), and even long-term low-dose intake can cause chronic poisoning. [...
Claims
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Login to View More IPC IPC(8): C07K14/415C12N15/29C12N15/70C12N15/81C12N15/84C12N1/21C12N1/19A01H5/00A01H6/20C12R1/645C12R1/19
CPCC07K14/415C12N15/70C12N15/815C12N15/8271
Inventor 王昌虎蔡兴喆区永祥
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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