67results about How to "Applicable to on-site inspection" patented technology

The invention provides a portable copper ion concentration detection method based on click chemistry and belongs to the field of analytical chemistry. Under the condition that sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-dipole cycloaddition reaction is carried out between NDA modified with an azide group and DNA modified with an alkynyl group, so that DNA modified with cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a cane sugar solution to carry out enzymatic hydrolysis, and then a reaction solution after enzymatic hydrolysis is measured by adopting a blood glucose meter, so that copper ion concentration can be detected. The portable copper ion concentration detection method provided by the invention takes the blood glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.

The invention discloses a method for detecting pesticide residues in sulfur-containing vegetables by an enzyme inhibition method. The method comprises the following steps of 1, preparing a standard solution, 2, preparing a sample, 3, testing control groups through the processes of adding a buffer solution into a beaker, adding dropwisely acetate or sodium hydroxide into the beaker, adjusting a pH value of the mixed solution to a pH value of 9, adding an enzyme solution and a color-developing agent into the mixed solution with a pH value of 9, shaking up, standing at a temperature of 37 DEG C for 15 minutes, adding a substrate solution into the mixed solution treated by the previous step, shaking up, and detecting an absorbance variation value delta A0 of the mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, 4, adding sulfur-containing vegetable sample extract into a beaker, carrying out processes same as the processes of the step 3, and detecting an absorbance variation value delta A1 of a vegetable sample mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, and 5, calculating an enzyme inhibition percentage according to a formula of an enzyme inhibition percentage=[(delta A0-delta A1)/delta A0]*100, wherein when the calculated enzyme inhibition percentage is great than or equal to 50%, it is shown that there are pesticide residues in the detected sulfur-containing vegetable. The method eliminates the interference of sulfides in a sulfur-containing vegetable on pesticide residue detection through adjustment of a pH value of a vegetable sample solution, can rapidly, simply and accurately detect a pesticide residue ratio of a sulfur-containing vegetable, has a low cost, and is suitable for on-site detection.

The invention discloses a portable method for detecting content of adenosine triphosphate (ATP). The method comprises the following steps of: respectively modifying a magnetic bead and invertase on an ATP aptamer which is split into two sections, performing specific identification and combination on the ATP aptamer which is split into two sections and a target ATP so as to form a sandwich structure, so that the aptamer modified with the invertase is fixed on the magnetic bead; separating the magnetic bead from the solution, dissolving the cleaned magnetic bead in a sucrose solution for carrying out an enzymatic hydrolysis reaction, measuring the reaction solution subjected to enzymatic hydrolysis by employing a glucometer, and indirectly detecting the concentration of ATP. The method is successfully applied to detection of the ATP concentration in a human serum sample, and the method has the advantages of simple operation, low cost, high sensitivity, high specificity and the like.

The invention relates to a method for rapidly detecting iodate by surface enhanced Raman spectroscopy and an application of the method. A Raman signal of the iodate is very weak, an SERS (Surface Enhanced Raman Scattering) signal of iodate is also weak, hydroxylamine is oxidated into nitrite by the iodate, coupling dye is generated from nitrite ions and a diazo-coupling reagent under an acidic or alkalescent condition, the SERS signal of the coupling dye is strong, and qualitative and quantitative detection of the iodate is indirectly carried out by measuring the dosage of the coupling dye through SERS. According to the method and the application thereof, Au/SiO2 is used as an SERS substrate material, the stability of particles is improved by means of a silicon dioxide shell, so that the result repeatability is better. The method is suitable for rapid detection of the iodate in complex samples, such as tablet salt, drinking water and environment water and has the advantages of simpleness and rapidness (about 2 minutes), suitability for field detection and benefits for popularization and use and the like.

The invention is suitable for the technical field of detection of novel coronavirus SARS-CoV-2, provides a primer and probe for normal-temperature and isothermal rapid detection of novel coronavirus SARS-CoV-2, and also provides a reagent, method and kit for normal-temperature and isotherma rapid detection of novel coronavirus SARS-CoV-2. The novel coronavirus SARS-CoV-2 can be detected rapidly and specifically in real time under the normal-temperature and isothermal conditions. Under the combined action of the specific primer, specific probe, five engineering enzymes and other chemical components, rapid detection of the target is achieved through nucleic acid colloidal gold test strips. No large-scale equipment is needed, a short detection time, convenient operation and high accuracy areachieved, result interpretation is simple and intuitive, and the primer, probe, reagent, method and kit are especially suitable for on-site detection.

The invention provides a method and a kit for on-site rapid detection of bovine-derived and duck-derived components, and belongs to the technical field of animal provenance component molecular detection. The kit provided by the invention comprises a combination of bovine and duck specific recombinase polymerase amplification (RPA) labeled primers and probes, and the sequences of the combination are shown as SEQ ID No.1-SEQ ID No.3 and SEQ ID No.4-SEQ ID No.6. The kit provided by the invention also comprises a detection test strip which can be used for carrying out multiple detection on RPA amplification products. When the combination of RPA primers and probes is adopted to amplify DNA of a sample, whether the sample contains bovine and duck-derived components or not can be judged by detecting amplification products by the test strip. The detection method provided by the invention has the advantages of high sensitivity, strong specificity, no need of special equipment and reaction timeof only 30 minutes. The invention provides an effective, accurate and reliable means for detection of bovine-derived and duck-derived components, has the characteristics of simplicity, convenience, rapidness and strong specificity, and is suitable for on-site detection.

The invention provides a hand-written surface-enhanced Raman scattering substrate, comprising a flexible substrate and metal nanoparticles distributed in the surface and interior of the flexible substrate, wherein the flexible substrate is paper or a polylactic acid film. The hand-written surface-enhanced Raman scattering substrate has the advantages of good enhancing effect, high stability, excellent detection consistency and low price, and is favorable for rapid analysis of a large number of samples in a laboratory and outdoor on-site detection. The invention also provides a preparation method for the hand-written surface-enhanced Raman scattering substrate. The preparation method comprises the following steps: providing a metal nanoparticle fluid suspension; filling a writing pen with the metal nanoparticle fluid suspension; and writing lines on the flexible substrate with the writing pen to allow the metal nanoparticles to be distributed in the surface and interior of the flexible substrate, thereby obtaining the hand-written surface-enhanced Raman scattering substrate. The preparation method is simple, does not need expensive advanced apparatuses and is suitable for large-scale production.

The invention discloses a method for improving a Sudan red I detection kit and belongs to the field of analytic chemistry. According to the method, a sample is pretreated to selectively enrich Sudan red I by a magnetic solid-phase extraction mode and then is detected by the Sudan red I detection kit. The method comprises the following steps of accurately weighing the sample, putting the sample into a container, adding an extraction solvent, oscillating and standing the container, and taking supernate as upper sample liquid; adding the upper sample liquid into a container holding a magnetic solid-phase extraction material, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding normal hexane, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding acetone, oscillating the container, dropwise adding the supernate on a sample application plate, and drying the sample application plate in a blowing manner; and executing other operations according to an operating manual of the detection kit. According to the method disclosed by the invention, the Sudan red I in the sample can be effectively and quickly enriched through magnetic solid-phase extraction, so that the detection limit of the kit is reduced by 3 times, and the phenomena of false positive and missing detection are avoided to an extremely large extent. The method has the advantages of simplicity, quickness, easiness in operation and the like, and is suitable for field test, and solvents can be saved.

The invention discloses a primer, a probe and a kit for detecting clonorchis sinensis through a RAA fluorescence method. The kit comprises the primer, the probe, a RAA fluorescence universal reactant,a reaction buffer solution, a negative quality control substance, and a positive quality control substance. The kit carries out isothermal amplification detection at a temperature of 30 to 42 DEG C,the detection is finished within 5 to 20 minutes; the operation is simple, and the kit can be co-used with a small instrument, is portable, has the characteristics of rapidness, sensitivity, accuracy,and good repeatability, is suitable for basic detection and on-site detection, and has a good application prospect.

The invention discloses a rapid quantitative detection method of cocaine suitable for field detection, comprising the following steps: (1), preparing a paper base detector: using paper as a detection base, outlining a hydrophilic detection area on the surface of the paper by using a hydrophobic barrier, and covalently coupling water-soluble up-conversion fluorescent nano-material and aptamer ACA-1; (2), surface modifying gold nanoparticles with aptamer ACA-2 to obtain solution AuNPs-ACA-2; (3), deeply optimizing the solution AuNPs-ACA-2; (4), preparing a standard solution of cocaine; (5), drawing a standard curve for cocaine detection; (6) measuring cocaine concentration in bodily fluid to be detected.The unsolved problem with rapid quantitative detection in the field of cocaine detection is solved, rapid, quantitative and highly sensitive detection of cocaine concentration in a bodily fluid sample is implemented, and quantitative detection sensitivity is up to 0.05 MuMu.

The invention provides an African swine fever virus nucleic acid rapid detection method and kit, and belongs to the technical field of biology. The kit provides specific RPA labeled primers ASF-F and ASF-R for detecting nucleic acid of the African swine fever virus, and the sequences of the specific RPA labeled primers ASF-F and ASF-R are shown as SEQ ID No.1 and SEQ ID No.2. The kit also provides a detection test strip which can be used for detecting RPA amplification products. The RPA primers are adopted to amplify sample DNA respectively, the amplification products are detected through the test strip, and whether the sample is infected with the African swine fever virus or not can be judged. The detection method established aiming at the nucleic acid of the African swine fever virus does not need special equipment, the reaction time is only 25 minutes, the sensitivity is up to 100 cp/microliter, no cross reaction exists in four common swine-origin viruses, and the specificity is high. The African swine fever virus nucleic acid rapid detection kit provides a simple and effective detection means for detecting the nucleic acid of the African swine fever virus, and is suitable for field detection.

The present invention discloses a seafood product detection kit, a preparation method and applications thereof. The test kit comprises: a test paper strip, wherein the test paper strip comprises a filtration pad, a sample pad, a detection pad and the like, a detection line and a quality control line are not coincident with each other, are distributed on the detection pad, and respectively contain a first antibody and a second antibody; and immunized nanometer silver gold core shell particles, wherein the immunized nanometer silver gold core shell particles contain functional polymer grafted nanometer silver gold core shell nanoparticles and a third antibody covalently bonded to a functional polymer, wherein the first antibody, a target and the third antibody are specifically bonded in a sandwich manner only in the presence of the target, and the second antibody and the third antibody are bonded in the presence or absence of the target. With the kit and the detection method of the present invention, the detection range and the detection sensitivity of the common food-borne pathogenic bacteria (especially Vibrio parahaemolyticus) in the seafood product can be substantially increased, the operation is simple, the detection time is short, and the favorable way is provided for the large batch and rapid detection of the seafood product.

The invention discloses a gas relay leakproofness detection device. The gas relay leakproofness detection device comprises a detection platform, a support is mounted on the detection platform, a clamping component for clamping a to-be-detected gas relay is mounted on the support, an oil filling component is mounted on the detection platform, connected with an oil filling port of the gas relay and used for filling the gas relay with oil and pressurizing the gas relay for leakproofness detection. The gas relay leakproofness detection device has the advantages of simple structure, small size, simplicity in operation and high detection efficiency.

The invention discloses an anti-infectious hematopoietic necrosis virus (IHNV) monoclonal antibody 6G7, and preparation and application thereof. The monoclonal antibody 6G7 is secreted by a hybridoma cell strain IHNV-6G7; the preservation number of the cell strain is CCTCC C201360. The hybridoma cell strain IHNV-6G7 can stably generate the monoclonal antibody 6G7. The antibody has the advantages of being high in valence, high in sensitivity, strong in specificity, strong in affinity with natural antigen and the like. The invention also provides a quick test strip containing the monoclonal antibody, and a kit thereof. By adopting a fluorescent nanometer microspheres immune chromatography detection technology, the monoclonal antibody 6G7 has the characteristics of being good in sensitivity, specificity, stability, repeatability, reproducibility and the like. Meanwhile, the test strip and the kit are simple and convenient to use, high in test speeds, and applicable to a field test; the testing requirements of food safety, slaughter, testing mechanisms and the like can be met.

The invention discloses a primer probe composition and probe for real-time fluorescence RAA detection of cyclosporidia DNA. The primer probe composition comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO.1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO.2. The nucleotide sequence of the probe is shown as SEQ ID NO. 7. In addition, the invention also discloses a kit containing the primer probe composition and application of the primer probe composition. The cyclosporidia can be rapidly and effectively detected, the detection accuracy, the specificity and the sensitivity are high, and the detection kit is sensitive, stable and effective.