67results about How to "Applicable to on-site inspection" patented technology

The invention provides a portable copper ion concentration detection method based on click chemistry and belongs to the field of analytical chemistry. Under the condition that sodium ascorbate reduces cupric into cuprous as a catalyst, a 1,3-dipole cycloaddition reaction is carried out between NDA modified with an azide group and DNA modified with an alkynyl group, so that DNA modified with cane sugar invertase is fixed on magnetic beads; The magnetic beads are separated from a solution, the cleaned magnetic beads are dissolved into a cane sugar solution to carry out enzymatic hydrolysis, and then a reaction solution after enzymatic hydrolysis is measured by adopting a blood glucose meter, so that copper ion concentration can be detected. The portable copper ion concentration detection method provided by the invention takes the blood glucose meter as a platform and can portably and quickly detect the copper ion concentration on site; and the portable copper ion concentration detection method can be applied to copper ion detection in a human body serum sample, and the portable copper ion concentration detection method has the advantages of easy operation, low cost, high sensitivity and good specificity.

The invention discloses a method for detecting pesticide residues in sulfur-containing vegetables by an enzyme inhibition method. The method comprises the following steps of 1, preparing a standard solution, 2, preparing a sample, 3, testing control groups through the processes of adding a buffer solution into a beaker, adding dropwisely acetate or sodium hydroxide into the beaker, adjusting a pH value of the mixed solution to a pH value of 9, adding an enzyme solution and a color-developing agent into the mixed solution with a pH value of 9, shaking up, standing at a temperature of 37 DEG C for 15 minutes, adding a substrate solution into the mixed solution treated by the previous step, shaking up, and detecting an absorbance variation value delta A0 of the mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, 4, adding sulfur-containing vegetable sample extract into a beaker, carrying out processes same as the processes of the step 3, and detecting an absorbance variation value delta A1 of a vegetable sample mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, and 5, calculating an enzyme inhibition percentage according to a formula of an enzyme inhibition percentage=[(delta A0-delta A1) / delta A0]*100, wherein when the calculated enzyme inhibition percentage is great than or equal to 50%, it is shown that there are pesticide residues in the detected sulfur-containing vegetable. The method eliminates the interference of sulfides in a sulfur-containing vegetable on pesticide residue detection through adjustment of a pH value of a vegetable sample solution, can rapidly, simply and accurately detect a pesticide residue ratio of a sulfur-containing vegetable, has a low cost, and is suitable for on-site detection.

The invention discloses a reagent, detection method and application for the detection of African hog cholera virus. The reagent comprises a specific primer pair and a probe, upstream and downstream primers of the primer pair are respectively sequences shown in Seq ID No. 1 and 2, and the probe is a sequence shown in Seq ID No. 3 or its reverse complement sequence; in the probe sequence shown in the SEQ ID No.3, a 28th base modifies fluorescent quenching group-dT, a 31st base is replaced with a base analog, a 33rd base modifies a fluorescent group-dT, and a 3' end modifies C3 Spacer. The reagent is sensitive, specific and efficient for detection of the African hog cholera virus by recombinase polymerase amplification; compared with conventional conventional or real-time fluorescent PCR, thereagent and the detection method have short detection time and simple operation, are especially suitable for on-site testing, and are of great significance for the rapid prevention and control of theAfrican hog cholera virus and guarantee of production safety.

The invention discloses a portable method for detecting content of adenosine triphosphate (ATP). The method comprises the following steps of: respectively modifying a magnetic bead and invertase on an ATP aptamer which is split into two sections, performing specific identification and combination on the ATP aptamer which is split into two sections and a target ATP so as to form a sandwich structure, so that the aptamer modified with the invertase is fixed on the magnetic bead; separating the magnetic bead from the solution, dissolving the cleaned magnetic bead in a sucrose solution for carrying out an enzymatic hydrolysis reaction, measuring the reaction solution subjected to enzymatic hydrolysis by employing a glucometer, and indirectly detecting the concentration of ATP. The method is successfully applied to detection of the ATP concentration in a human serum sample, and the method has the advantages of simple operation, low cost, high sensitivity, high specificity and the like.

The invention relates to a method for rapidly detecting iodate by surface enhanced Raman spectroscopy and an application of the method. A Raman signal of the iodate is very weak, an SERS (Surface Enhanced Raman Scattering) signal of iodate is also weak, hydroxylamine is oxidated into nitrite by the iodate, coupling dye is generated from nitrite ions and a diazo-coupling reagent under an acidic or alkalescent condition, the SERS signal of the coupling dye is strong, and qualitative and quantitative detection of the iodate is indirectly carried out by measuring the dosage of the coupling dye through SERS. According to the method and the application thereof, Au / SiO2 is used as an SERS substrate material, the stability of particles is improved by means of a silicon dioxide shell, so that the result repeatability is better. The method is suitable for rapid detection of the iodate in complex samples, such as tablet salt, drinking water and environment water and has the advantages of simpleness and rapidness (about 2 minutes), suitability for field detection and benefits for popularization and use and the like.

The invention discloses an ionic liquid functional composite membrane modified electrode and a preparation method and application thereof to detection of chlorophenol. Special dissolution and high electrical conductivity of ionic liquid are utilized, amino functional ionic liquid conducts epoxide ring-opening on an oxidized graphene-gold nanoparticle composite, the ionic liquid functional graphene-gold nanoparticle composite is prepared, and a dispensing method is adopted to prepare the corresponding composite membrane modified electrode. The surface of the obtained modified electrode has the advantages of being large in effective area and good in dispersibility, and many active sites exist; the synergistic effect of ionic liquid, graphene and gold nanoparticles is played on the aspect of improving direct electrochemistry and electro-catalytic performance, and electrical conductivity and catalytic performance of the modified electrode are improved. An obtained chlorophenol sensor based on the composite membrane modified electrode has the advantages of being low in detection limit, wide in detection range, high in response speed and the like.

The invention is suitable for the technical field of detection of novel coronavirus SARS-CoV-2, provides a primer and probe for normal-temperature and isothermal rapid detection of novel coronavirus SARS-CoV-2, and also provides a reagent, method and kit for normal-temperature and isotherma rapid detection of novel coronavirus SARS-CoV-2. The novel coronavirus SARS-CoV-2 can be detected rapidly and specifically in real time under the normal-temperature and isothermal conditions. Under the combined action of the specific primer, specific probe, five engineering enzymes and other chemical components, rapid detection of the target is achieved through nucleic acid colloidal gold test strips. No large-scale equipment is needed, a short detection time, convenient operation and high accuracy areachieved, result interpretation is simple and intuitive, and the primer, probe, reagent, method and kit are especially suitable for on-site detection.

The invention discloses a photometric method based boat-carrying pH and pCO2 measuring device and measuring method. The measuring device comprises a pretreatment apparatus and a measuring apparatus. The pretreatment apparatus comprises a submerged pump, a seawater collecting pipeline, a seawater discharging pipeline, a seawater sample tank, a filter head, and a first thermostatic water bath. The measuring apparatus comprises a seawater sample injection pipeline, an indicator bag, an indicator sample injection pipeline, a three-way valve, a peristaltic pump, a mixer, a Teflon AF2400 pipeline, a flow-through cell, a waste discharging pipeline, a miniature deuterium-tungsten halogen lamp, a spectrometer, an air thermotank, and a second thermostatic water bath. The provided measuring device can be used to measuring seawater pH and pCO2. In the prior art, the measurement on the sea carbonate system is complicated and tedious, the power consumption cost is high, and the provided device can solve the problem. Furthermore, the measuring device uses the photometric method, thus the measurement precision is high, and the measuring device can be widely applied to sensors.

The invention provides a method and a kit for on-site rapid detection of bovine-derived and duck-derived components, and belongs to the technical field of animal provenance component molecular detection. The kit provided by the invention comprises a combination of bovine and duck specific recombinase polymerase amplification (RPA) labeled primers and probes, and the sequences of the combination are shown as SEQ ID No.1-SEQ ID No.3 and SEQ ID No.4-SEQ ID No.6. The kit provided by the invention also comprises a detection test strip which can be used for carrying out multiple detection on RPA amplification products. When the combination of RPA primers and probes is adopted to amplify DNA of a sample, whether the sample contains bovine and duck-derived components or not can be judged by detecting amplification products by the test strip. The detection method provided by the invention has the advantages of high sensitivity, strong specificity, no need of special equipment and reaction timeof only 30 minutes. The invention provides an effective, accurate and reliable means for detection of bovine-derived and duck-derived components, has the characteristics of simplicity, convenience, rapidness and strong specificity, and is suitable for on-site detection.

The invention relates to a portable tester for a time response of a merging unit. The tester comprises a microprocessor, a field programmable gate array, an optical fiber emitter, a FLASH peripheral chip and interface hardware, such as an LCD (Liquid Crystal Display), an Ethernet port and a keyboard. When the tester is used for testing the merging unit, a time tick message is sent by the optical fiber emitter through an optical fiber pulse per second or optical fiber IRIG-B code, at the moment, the output from the merging unit is received by the tester, and the rated delay, time jitter degree and synchronous punctuality of the merging unit are tested by the tester. A configured FT3 signal of a collector or PT merging unit is sent by the tester, the output from the merging unit is received by the tester, and the absolute delay and cascaded delay of the merging unit are tested by the tester. The portable tester for the time response of the merging unit has ultra-strong adaptation, is real-time and quick, is portable and is fit for the field test for the time response of the merging unit.

The invention discloses an elevator traction wheel sliding detection device and method. The elevator traction wheel sliding detection device comprises a traction wheel angular displacement measuring device, a speed limiter angular displacement measuring device and a signal processing system, wherein the traction wheel angular displacement measuring device and the speed limiter angular displacementmeasuring device are electrically connected with the signal processing system, the traction wheel angular displacement measuring device is fixedly connected to an elevator frame, and the traction wheel angular displacement measuring device, the speed limiter angular displacement measuring device and the signal processing system are all arranged on an iron plate at the top of an elevator machine room. The traction wheel angular displacement measuring device and the speed limiter angular displacement measuring device are used to calculate the slippage amount between a traction wheel and a steelwire rope, and the measurement belongs to non-contact measurement so that the defect of direct contact measurement can be overcome; and long-time and all-weather automatic detection of the slippage amount can be realized conveniently, and a scientific basis is provided for scientifically judging whether the traction wheel loses efficacy or not.

The invention discloses a recombinant enzyme amplification detection method for HPV16 and HPV18 genotypes, a test strip and application thereof. The sequences of a primer pair for the HPV16 genotype are shown in SEQ ID NO.1 and SEQ ID NO.2 respectively. The sequences of a primer pair for the HPV18 genotype are shown as SEQ ID NO.3 and SEQ ID NO.4 respectively. The double lateral flow chromatography test strip comprises a supporting plate, wherein the upper end face of the supporting plate comprises a sample loading zone, a labeling zone, a marking-off zone and a water absorption zone which arearranged in sequence; the labeling zone is bound with latex microspheres coupled with streptavidin, and detection lines T1 and T2 and a quality control line C which are bound with an FITC antibody, adigoxin and a second antibody respectively. The detection method is good in specificity, high in sensitivity, good in specificity, good in repeatability, easy to operate and high in applicability.

The invention relates to a fast water-injected meat detection method. The method comprises the steps of detecting a meat product by utilizing a fast electrical meat impedance-spectroscopy detection instrument to obtain electrical bioimpedance-spectroscopy data, obtaining the parameters R0, R[infinite], a and f[C] of a Cole impedance model by adopting an iterative least squares fitting method, acquiring an output value of a classification forecasting model by taking the parameters R0, R[infinite], alpha and f[C] of a Cole impedance model as input features of an artificial neural network classification forecasting model, and judging whether the detected meat product is water-injected meat according to the output value. According to the detection method, by adopting a two-electrode method, an alternating-current signal with constant amplitude value is inserted in the detected meat product through a pair of needle-like electrodes, and the change of voltages at two ends can be detected through the same pair of electrodes, so that the detection data can be obtained, the fast detection on the meat product can be realized without the damage to the detected sample, and the detection precision is high.

The invention provides a hand-written surface-enhanced Raman scattering substrate, comprising a flexible substrate and metal nanoparticles distributed in the surface and interior of the flexible substrate, wherein the flexible substrate is paper or a polylactic acid film. The hand-written surface-enhanced Raman scattering substrate has the advantages of good enhancing effect, high stability, excellent detection consistency and low price, and is favorable for rapid analysis of a large number of samples in a laboratory and outdoor on-site detection. The invention also provides a preparation method for the hand-written surface-enhanced Raman scattering substrate. The preparation method comprises the following steps: providing a metal nanoparticle fluid suspension; filling a writing pen with the metal nanoparticle fluid suspension; and writing lines on the flexible substrate with the writing pen to allow the metal nanoparticles to be distributed in the surface and interior of the flexible substrate, thereby obtaining the hand-written surface-enhanced Raman scattering substrate. The preparation method is simple, does not need expensive advanced apparatuses and is suitable for large-scale production.

The invention discloses a method for improving a Sudan red I detection kit and belongs to the field of analytic chemistry. According to the method, a sample is pretreated to selectively enrich Sudan red I by a magnetic solid-phase extraction mode and then is detected by the Sudan red I detection kit. The method comprises the following steps of accurately weighing the sample, putting the sample into a container, adding an extraction solvent, oscillating and standing the container, and taking supernate as upper sample liquid; adding the upper sample liquid into a container holding a magnetic solid-phase extraction material, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding normal hexane, oscillating the container, arranging a magnet on the container, and pouring out the supernate; adding acetone, oscillating the container, dropwise adding the supernate on a sample application plate, and drying the sample application plate in a blowing manner; and executing other operations according to an operating manual of the detection kit. According to the method disclosed by the invention, the Sudan red I in the sample can be effectively and quickly enriched through magnetic solid-phase extraction, so that the detection limit of the kit is reduced by 3 times, and the phenomena of false positive and missing detection are avoided to an extremely large extent. The method has the advantages of simplicity, quickness, easiness in operation and the like, and is suitable for field test, and solvents can be saved.

The invention discloses a primer, a probe and a kit for detecting clonorchis sinensis through a RAA fluorescence method. The kit comprises the primer, the probe, a RAA fluorescence universal reactant,a reaction buffer solution, a negative quality control substance, and a positive quality control substance. The kit carries out isothermal amplification detection at a temperature of 30 to 42 DEG C,the detection is finished within 5 to 20 minutes; the operation is simple, and the kit can be co-used with a small instrument, is portable, has the characteristics of rapidness, sensitivity, accuracy,and good repeatability, is suitable for basic detection and on-site detection, and has a good application prospect.

The invention discloses a rapid quantitative detection method of cocaine suitable for field detection, comprising the following steps: (1), preparing a paper base detector: using paper as a detection base, outlining a hydrophilic detection area on the surface of the paper by using a hydrophobic barrier, and covalently coupling water-soluble up-conversion fluorescent nano-material and aptamer ACA-1; (2), surface modifying gold nanoparticles with aptamer ACA-2 to obtain solution AuNPs-ACA-2; (3), deeply optimizing the solution AuNPs-ACA-2; (4), preparing a standard solution of cocaine; (5), drawing a standard curve for cocaine detection; (6) measuring cocaine concentration in bodily fluid to be detected.The unsolved problem with rapid quantitative detection in the field of cocaine detection is solved, rapid, quantitative and highly sensitive detection of cocaine concentration in a bodily fluid sample is implemented, and quantitative detection sensitivity is up to 0.05 MuMu.

The invention belongs to the field of food detection and especially relates to acid value rapid test paper and a detection method thereof. A production method of the rapid test paper comprises the following steps: (1) preparing a soak solution: putting 0.01-2g of methyl violet, 0.02-1.5 g of methyl red and 0.02-2g of Congo red in a 1000mL beaker, adding 1000mL of absolute ethyl alcohol, and fullystirring and dissolving to obtain the soak solution; and (2) taking glass cellulose paper, cutting the glass cellulose paper into strips, soaking the strips in the soak solution for 20-30 minutes in adark place, taking out the strips, drying the strips under a vacuum condition of 45-60 DEG C, cutting the dried test strips into test paper sheets of 6*6mm, and adhering the test paper sheets to oneends of PVC strips so as to obtain the rapid test paper. The method realizes one-step detection, does not need steps of titration, calculation and the like, greatly saves detection time and is especially suitable for on-site detection.

The invention provides a primer for rapidly detecting respiratory tract adenoviruses based on an RAA fluorescent method, a probe and a detection kit. The primer and the probe are suitable for RAA fluorescent method detection and can accurately detect the respiratory tract adenoviruses, the specificity can reach 100%, and the detection kit can conveniently, rapidly and accurately authenticate the respiratory tract adenoviruses. Operation is convenient, detection time is short, detection can be completed within 15 min, it is unnecessary to make DNAunqinding at the high temperature of 95 DEG C like PCR, then conduct annealing at the temperature of 50-60 DEG C and finally complete extending at the temperature of 72 DEG C, it is only needed to conduct isothermal amplification at the temperatureof 30-42 DEG C, and then detection is completed. It is also unnecessary to use 4-6 primers for amplification at the temperature of 65 DEG C like an LAMP technology, and false positive is not easily generated.

The invention provides an African swine fever virus nucleic acid rapid detection method and kit, and belongs to the technical field of biology. The kit provides specific RPA labeled primers ASF-F and ASF-R for detecting nucleic acid of the African swine fever virus, and the sequences of the specific RPA labeled primers ASF-F and ASF-R are shown as SEQ ID No.1 and SEQ ID No.2. The kit also provides a detection test strip which can be used for detecting RPA amplification products. The RPA primers are adopted to amplify sample DNA respectively, the amplification products are detected through the test strip, and whether the sample is infected with the African swine fever virus or not can be judged. The detection method established aiming at the nucleic acid of the African swine fever virus does not need special equipment, the reaction time is only 25 minutes, the sensitivity is up to 100 cp / microliter, no cross reaction exists in four common swine-origin viruses, and the specificity is high. The African swine fever virus nucleic acid rapid detection kit provides a simple and effective detection means for detecting the nucleic acid of the African swine fever virus, and is suitable for field detection.

The invention discloses a glass fiber filter paper-based viral nucleic acid extraction method. The method comprises the following steps of 1, adding protease K in a biological sample and carrying outrapid mixing to obtain mixed solution; 2, adding binding buffer and Carrier RNA and turning upside down to sufficiently mixing the materials; 3, dropwise adding the mixed solution on glass fiber filter paper, fixing the glass fiber filter paper by taking double-loop filter paper as a support, and absorbing the filtered waste liquid by using absorbent paper; 4, carrying out cleaning by using washing buffer and absorbing the waste liquid by using absorbent paper; and 5, dropwise adding eluting buffer on the glass fiber filter paper, and collecting eluent to obtain required nucleic acid. According to the method, the glass fiber filter paper is imported into the nucleic acid extraction method and can be used for the enrichment and extraction of nucleic acid in biological samples. The method iscapable of simplifying the nucleic acid extraction step and the required instrument equipment, thereby saving the operation time, reducing the extraction cost and ensuring the high-efficiency nucleicacid recovery rate at the same time.

The present invention discloses a seafood product detection kit, a preparation method and applications thereof. The test kit comprises: a test paper strip, wherein the test paper strip comprises a filtration pad, a sample pad, a detection pad and the like, a detection line and a quality control line are not coincident with each other, are distributed on the detection pad, and respectively contain a first antibody and a second antibody; and immunized nanometer silver gold core shell particles, wherein the immunized nanometer silver gold core shell particles contain functional polymer grafted nanometer silver gold core shell nanoparticles and a third antibody covalently bonded to a functional polymer, wherein the first antibody, a target and the third antibody are specifically bonded in a sandwich manner only in the presence of the target, and the second antibody and the third antibody are bonded in the presence or absence of the target. With the kit and the detection method of the present invention, the detection range and the detection sensitivity of the common food-borne pathogenic bacteria (especially Vibrio parahaemolyticus) in the seafood product can be substantially increased, the operation is simple, the detection time is short, and the favorable way is provided for the large batch and rapid detection of the seafood product.

The invention discloses a gas relay leakproofness detection device. The gas relay leakproofness detection device comprises a detection platform, a support is mounted on the detection platform, a clamping component for clamping a to-be-detected gas relay is mounted on the support, an oil filling component is mounted on the detection platform, connected with an oil filling port of the gas relay and used for filling the gas relay with oil and pressurizing the gas relay for leakproofness detection. The gas relay leakproofness detection device has the advantages of simple structure, small size, simplicity in operation and high detection efficiency.

The invention discloses a method for rapidly, simply and conveniently detecting illegal cooking oil. The method comprises the following steps of adding a nonionic surfactant into a selected to-be-detected oil sample, adequately and uniformly mixing to form sample oil-nonionic surfactant mixed liquid, adding the sample oil-nonionic surfactant mixed liquid into deionized water to be emulsified so that sample oil-nonionic surfactant mixed liquid is uniformly dispersed to form milky white liquid, measuring the electrical conductivity of the milky white liquid, and comparing the electrical conductivity of the milky white liquid with electrical conductivity of the qualified edible oil measured under the same conditions, so as to judge whether the to-be-detected oil sample is the illegal cooking oil or not. The method for rapidly, simply and conveniently detecting the illegal cooking oil has the beneficial effects that (1) solvents, particularly chemical solvents such as methanol, diethyl ether or petroleum ether are not required; (2) the illegal cooking oil can be directly detected without layered processing or layered l extraction; (3) all small molecular substances of the illegal cooking oil are detected, so that a result is accurate; (4) the detection method is high in detection speed and is applicable to field detection; (5) the technique is simple and convenient and is suitable for the use of consumers of public families; (6) the technical accuracy is high, and a small amount of the illegal cooking oil mixed in the edible oil can be detected.

The invention discloses a primer composition, a kit and a method for rapidly detecting poisonous mushroom russule japonica based on LAMP. The primer composition comprises a forward inner primer FIP, a reverse inner primer BIP, a forward outer primer F3, a reverse outer primer B3 and a loop primer LB. The LAMP primer composition, the kit and the method have very high specificity, can quickly, accurately, sensitively and conveniently detect whether a sample is toxic russule or not, are easy to distinguish from russule delicious which is eaten by ordinary people at ordinary times, can meet the requirements of basic disease control and medical units for quickly identifying a poison source after mushroom poisoning and can effectively treat and cure a patient, and excessive waste of medical resources is avoided.

The invention relates to a multi-working mode calibrator for an aircraft standby compass. The multi-working mode calibrator comprises a housing as well as a partition plate, a magnetic sensor, a horizontal angle sensor, a processor module, a compass rotation knob, an observation window rotation knob, a magnetic heading cursor rotation knob, an observation window locking button, a horizontal anglesensor orientation button, a reset switch, a rechargeable battery, a power source switch, a charging interface, a raspberry pie module and an electronic display which are all mounted on the housing. The multi-working mode calibrator of the invention has the working modes of two kinds of existing aircraft compass calibrators; and corresponding device operation methods are consistent; the two working modes can be mutually switched. The multi-working mode calibrator of the invention has the advantages of short calibration time, low maintenance cost, high portability, simple equipment, convenientoperation and high detection speed. Compared with a mechanical compass deviation azimuth instrument, the multi-working mode calibrator is provided with digital devices, and therefore, mechanical errorcan be decreased; and error can be calibrated in time through an algorithm, so that the multi-working mode calibrator has high operational repeatability and is low in cost during update. The multi-working mode calibrator is just slightly influenced by surrounding environments and is very suitable for on-site inspection.

The invention discloses an anti-infectious hematopoietic necrosis virus (IHNV) monoclonal antibody 6G7, and preparation and application thereof. The monoclonal antibody 6G7 is secreted by a hybridoma cell strain IHNV-6G7; the preservation number of the cell strain is CCTCC C201360. The hybridoma cell strain IHNV-6G7 can stably generate the monoclonal antibody 6G7. The antibody has the advantages of being high in valence, high in sensitivity, strong in specificity, strong in affinity with natural antigen and the like. The invention also provides a quick test strip containing the monoclonal antibody, and a kit thereof. By adopting a fluorescent nanometer microspheres immune chromatography detection technology, the monoclonal antibody 6G7 has the characteristics of being good in sensitivity, specificity, stability, repeatability, reproducibility and the like. Meanwhile, the test strip and the kit are simple and convenient to use, high in test speeds, and applicable to a field test; the testing requirements of food safety, slaughter, testing mechanisms and the like can be met.

The invention discloses a primer probe composition and probe for real-time fluorescence RAA detection of cyclosporidia DNA. The primer probe composition comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO.1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO.2. The nucleotide sequence of the probe is shown as SEQ ID NO. 7. In addition, the invention also discloses a kit containing the primer probe composition and application of the primer probe composition. The cyclosporidia can be rapidly and effectively detected, the detection accuracy, the specificity and the sensitivity are high, and the detection kit is sensitive, stable and effective.

According to a florfenicol quick detection kit, the colloidal gold is taken as a marker. The competitive inhibition principle is adopted, so that a high-sensitivity florfenicol colloidal gold immunochromatography quantitative detection method is established. According to the invention, a colloidal gold immunochromatography technology is utilized; a nitrocellulose membrane is coated with a florfenicol antigen (test line) and goat anti-mouse IgG (quality control line), a labeled florfenicol gold-labeled antibody is sprayed on a gold-labeled pad, when a treated egg sample flows through an NC membrane, a red line is displayed on the NC membrane, and whether the sample contains florfenicol or not is judged according to whether a detection line (T line) exists or not.