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Primer composition, kit and method for rapidly detecting poisonous mushroom russule japonica based on LAMP

The technology of a primer composition and a kit, which is applied in the field of rapid detection of toxic organisms, achieves the effects of short time consumption, avoiding excessive waste, and simple reaction operation

Active Publication Date: 2022-01-18
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, there are no reports and patents about the rapid detection of poisonous russula russula japonicus both at home and abroad

Method used

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  • Primer composition, kit and method for rapidly detecting poisonous mushroom russule japonica based on LAMP
  • Primer composition, kit and method for rapidly detecting poisonous mushroom russule japonica based on LAMP
  • Primer composition, kit and method for rapidly detecting poisonous mushroom russule japonica based on LAMP

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Embodiment

[0034] A primer composition based on LAMP for rapid detection of poisonous mushroom Russula japonicus, comprising: forward inner primer FIP, reverse inner primer BIP, forward outer primer F3, reverse outer primer B3 and loop primer LB; the base sequence is as follows :

[0035] FIP: 5'-AGCTGGCTTTGAGAGGAAAGCTAATGGTTTCTTGATCAAGAAGGC-3' (represented by SEQ ID NO: 1);

[0036] BIP: 5'-TAGTAGGGTCTACTTTGCTGATCCTATAGGTTTGCAAAAGCCCAAG-3' (shown by SEQ ID NO: 2);

[0037] F3: 5'-TGTCGTGATATCTTCAACCTT-3' (shown by SEQ ID NO:3);

[0038] B3: 5'-AGTCTCTGACGAGACAGTT-3' (shown by SEQ ID NO:4);

[0039] LB: 5'-AGCAAAAGCCTCCAAATTCCAA-3' (represented by SEQ ID NO:5).

[0040]Russula genus materials such as Russula japonica were collected in the wild, and the specimens were collected in the Mycological Hebarium of Hunan Normal University (MHHNU).

[0041] The specific operation steps of Japanese red mushroom LAMP isothermal rapid detection are as follows:

[0042] A. DNA extraction of Russ...

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Abstract

The invention discloses a primer composition, a kit and a method for rapidly detecting poisonous mushroom russule japonica based on LAMP. The primer composition comprises a forward inner primer FIP, a reverse inner primer BIP, a forward outer primer F3, a reverse outer primer B3 and a loop primer LB. The LAMP primer composition, the kit and the method have very high specificity, can quickly, accurately, sensitively and conveniently detect whether a sample is toxic russule or not, are easy to distinguish from russule delicious which is eaten by ordinary people at ordinary times, can meet the requirements of basic disease control and medical units for quickly identifying a poison source after mushroom poisoning and can effectively treat and cure a patient, and excessive waste of medical resources is avoided.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of toxic organisms, and in particular relates to a primer composition, a kit and a method for rapid detection of poisonous russula russula japonicus based on a loop-mediated isothermal amplification (LAMP) technology. Background technique [0002] Russula japonica belongs to Basidiomycota, Agaricomycetes, Russulales, Russulaceae and Russula in classification. Russula russula is the species of the genus that is most likely to be eaten by ordinary people. It is very similar to the delicious red mushroom that ordinary people usually eat, and it cannot be distinguished through life experience. According to the 2019 and 2020 China Center for Disease Control and Prevention's annual investigation report on mushroom poisoning, 56 species of species that cause gastroenteritis symptoms were identified based on the poisoning cases. As a result, 289 people were poisoned, and the number of poisoning c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6844C12N15/11C12R1/93
CPCC12Q1/6895C12Q1/6844C12Q2531/119C12Q2565/113C12Q2565/125Y02A50/30
Inventor 陈作红龙攀何正蜜张平
Owner HUNAN NORMAL UNIVERSITY
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