37results about How to "Convenient, fast and accurate detection" patented technology

Method of manufacturing a three-dimensional object according to which the object is built layer-wise by solidification of a building material, wherein a test specimen is built which is excited to oscillate after being built and wherein natural frequencies of the oscillations are determined.

The invention discloses a monoclonal antibody against human MxA. The monoclonal antibody against the human MxA is generated by a hybridoma cell line CCTCC NO: C200916, and the monoclonal antibody is of IgG1 subtype and kappa type; the monoclonal antibody has good specificity and high affinity, can identify a prokaryotic expression product of the human MxA, an eukaryotic expression product of the human MxA, interferon or human MxA gene expression products induced by other factors, simultaneously can identify denatured protein and non-denatured protein of MxA expression protein, and well reflects the existence form of a polymer of the protein; therefore, the monoclonal antibody can simply, conveniently, quickly and accurately detect the expression level of the human MxA protein.

The invention discloses a primer, a probe and a kit for detecting clonorchis sinensis through a RAA fluorescence method. The kit comprises the primer, the probe, a RAA fluorescence universal reactant,a reaction buffer solution, a negative quality control substance, and a positive quality control substance. The kit carries out isothermal amplification detection at a temperature of 30 to 42 DEG C,the detection is finished within 5 to 20 minutes; the operation is simple, and the kit can be co-used with a small instrument, is portable, has the characteristics of rapidness, sensitivity, accuracy,and good repeatability, is suitable for basic detection and on-site detection, and has a good application prospect.

The invention discloses a high-precision head lamp assembly detecting tool, comprising a base (1), wherein a frame constructed according to surrounding metal plate data of a head lamp assembly of an actual vehicle to place the head lamp assembly and a positioning block for simulating installation of the actual vehicle to fix the head lamp assembly are arranged on the base (1); the frame is composed of a turnover mechanism I (3) and a turnover mechanism II (4) on the upper side as well as a turnover mechanism III (5), a turnover mechanism IV (6) and a fixed profile (8) on the lower side; and the turnover mechanism I (3), the turnover mechanism II (4), the turnover mechanism III (5) and the turnover mechanism IV (6) are all of rotary openable structures. The stimulated actual head lamp is matched with the vehicle body, so that a tail lamp assembly can be conveniently, quickly and accurately detected; the accuracy of mounting holes positions, the plane difference fit precision and the clearance fit precision can be detected, and the unqualified parts can be adjusted and modified in time according to the detection data, so that the quality of the product is improved and the attractiveness of the assembled vehicle is promoted.

The invention discloses a primer, probe and detection method for detecting pasteurella multocida by an RAA fluorescence method. The primer and the probe are suitable for detection by the RAA fluorescence method, can accurately detect pasteurella multocida plasmids and positive samples, and do not have cross reactions with mycoplasma, streptococcus, escherichia coli, eperythrozoon, neospora caninum, toxoplasma gondii and healthy cow blood, and the specificity reaches 100%. The detection method is quick, and high flux is easy to realize, besides, the detection time is reduced, and the detectioncost is reduced. A method for quickly detecting pasteurella multocida DNA based on the RAA fluorescence method, provided by the invention, is high in sensitivity, and the reaction detection sensitivity reaches 10copies/[mu] L.

The invention discloses a primer pair, a probe, a kit and a method for fluorescence quantitative PCR detection of murine encephalomyocarditis viruses. The detection method takes cDNA of brain issues of a mouse as a template, SEQ ID No:1 and SEQ ID No:2 as primers and SEQ ID No:3 as the probe to perform fluorescence quantitative RT-PCR amplification and acquire a fluorescence signal. The fluorescence quantitative RT-PCR detection method disclosed by the invention can realize quick, accurate, convenient, rapid and specific detection of murine encephalomyocarditis virus nucleic acids in a mouse body, and the sensitivity, the repeatability and the specificity can be ensured.

The invention discloses a primer and a probe for detecting Nipah viruses by an RAA fluorescence method as well as a detection method, wherein the primer and the probe are suitable for RAA fluorescencemethod detection, Nipah virus plasmid can be detected, cross reaction with Hendra virus plasmid, foot and mouth disease viruses, porcine parvoviruses, pseudorabies viruses, porcine circoviruses, epidemic type b encephalitis viruses, reproductive and respiratory syndrome viruses, hog cholera viruses and infectious gastroenteritis viruses can be avoided, and the specificity reaches to 100 percent;the detection method is rapid, high flux is easy to realize, the detection time and the detection cost are reduced; and according to the method for rapidly detecting Nipah virus DNA based on the RAA fluorescence method, the sensitivity is high, and 74 copies/reaction is realized under the probability that the reaction detection sensitivity is 95 percent.

The invention provides a method and culture medium for specifically detecting sclerotinia sclerotiorum through 3-(2-pyridyl) methyl-2-(4-chlorphenyl) imino group thiazolidine or a derivative thereof with an imido-thiazolidine structure. The method provided by the invention can be used for specifically inducing the mycelia of the sclerotinia sclerotiorum to generate outstanding green pigments in a culture medium shake flask or a flat plate which contains 0.1-100.0 micrograms/milliliter of a detection reagent and has the characteristics of high specificity and high sensitivity and stability. The method comprises the following steps: ultrasonically leaching the green mycelia by using 90% ethanol; and then regulating pH to 3.0 by using 0.1 M of HCl to obtain the green pigments, wherein the green pigments have characteristic absorption peaks in a region with the wavelength of 610-630 nanometers. The detection reagent has certain prevention and control effects on the sclerotinia rot of colza.

The invention discloses primers, probe and detecting method for detecting giardia lamblia through an RAA fluorescence method. The primes, probe and detecting method have high specificity, sensitivityand repeatability, the giardia lamblia can be conveniently, rapidly and accurately authenticated, operation is simple and convenient, the detection time is short, and detection can be completed within15 min. The high flux can be rapidly and easily realized through the detecting method, the detection cost is greatly reduced, sensitivity is high, and the sensitivity of each time of reaction detection reaches 100 Copies per reaction.

The invention provides a fluorogenic quantitative PCR detecting method of vibrios. The method comprises the steps that DNA of bacterial colonies obtained from culturing sample bacteria is extracted, fluorogenic PCR amplification is carried out on the DNA of the bacterial colonies through specific primers, and the specific primers are one of toxR gene primer pairs indicated by a sequence table SEQ ID No.1-2, flaA gene primer pairs indicated by a sequence table SEQ ID No.3-4, and pyrH gene primer pairs indicated by a sequence table SEQ ID No.5-6. The method is high in specificity and sensitivity, the pathogenic bacterium variety can be further determined as vibrio fluvialis or vibrio anguillarum or vibrio alginolyticus, the minimum concentration capable of being detected is 1.0*10<-6> nmol/L, 1.0*10<-7> nmol/L and 1.0*10<-8> nmol/L, detecting is convenient, fast and accurate, and great significance in preventing vibriosis, one of main bacterial diseases of maricultural animals, is achieved.

The invention discloses a taillight assembly detector. The detector comprises a pedestal (1), the pedestal is provided with a frame and positioning blocks and points for fixing a taillight assembly after simulating practical automobile assembly and installation, and the frame is established according to sheet metal data in the circumference of taillights of real vehicle and used for placing the taillight assembly; the frame is composed of an upper profile (3) and a first overture mechanism (4) in the upper side, a first sliding mechanism (5) and a second sliding mechanism (6) in the lower side and a lateral profile fixed at one side of the first overture mechanism (4) and the first sliding mechanism (5); the upper profile (3) is connected with the second sliding mechanism (6); and the first overture mechanism (4), the first sliding mechanism (5) and the second sliding mechanism (6) are of movable adjustable structures respectively. According to the invention, practical vehicle assembly is simulated, detection is carried out conveniently, rapidly and accurately, the accuracy of the positions of installation holes, the surface difference and gaps are detected, and the unqualified condition can be modified according to detection data to improve the quality of products.

The invention belongs to the technical field of aquaculture oxygenation and in particular relates to a wave pushing and water stirring machine. The wave pushing and water stirring machine comprises afloating ring, wherein the floating ring is fixedly provided with a plurality of brackets; a water pulling plate is arranged at one end of each bracket and a fixed seat is arranged at the other end ofeach bracket; the fixed seat is arranged at the center of the floating ring; the fixed seat is provided with a first motor; the first motor comprises a machine shell and a main shaft; the fixed seatsleeves the main shaft; a limiting seat sleeves the other side of the machine shell; the limiting seat is used for preventing the machine shell and the main shaft from rotating at the same time through a fixed rod. The invention aims at providing the wave pushing and water stirring machine which has the advantages of stable structure, high oxygenation efficiency, long service life and various functions.

The invention provides a kit for detecting four psychiatric drugs or metabolites thereof in an in-vivo biological sample, wherein the kit comprises: (1) a mixed standard solution TDM detection tube, of which the preparation method comprises: adding an internal standard solution and a mixed standard solution into the TDM detection tube, and drying the tube; and (2) a sample solution detection TDM detection tube, of which a preparation method comprises the following steps: adding the internal standard solution into the TDM detection tube, and drying the tube; (3) a reconstitution solution being a methanol-water solution containing formic acid; (4) a specification, recording a use method of the kit. The kit is stored at the temperature of -20 to -15 DEG C. Liquid-liquid extraction and an internal standard UPLC-UV method are adopted, and separation of common psychiatric drugs and active metabolites thereof in human serum or hair is achieved; the mobile phase is free of salt additives, so that switching of different methods is facilitated. Therefore, the kit has good clinical detection value and economic significance while the detection time is greatly shortened, and has good application prospects.

The invention relates to a hole depth detection device of an automobile brake system bracket. The hole depth detection device comprises a clamping and positioning part and a detecting part, wherein the clamping and positioning part comprises a bottom plate (1), a positioning plate (2), an X-direction tightening pin (3) and a Y-direction positioning column (4); and the detection part comprises a detection column (5). The bottom plate (1) is provided with the positioning plate (2), the Y-direction positioning column (4) and the X-direction tightening pin (3), and used for positioning and clamping a detected workpiece. The detection column (5) can be inserted downward into a detection hole corresponding to the position of a detected workpiece hole, the upper end of the detection column (5) is engraved with a graduated range so as to confirm whether the hole depth is qualified or not. The hole depth detection device provided by the invention has the characteristics of convenient detection, accurate data, easy operation and the like, has an actual using effect for detection of the automobile brake system bracket hole depth, enables the production and detection efficiency of the automobile brake system bracket to be improved greatly, and greatly reduces the processing reject ratio of the automobile brake system bracket.

The invention discloses a snow density detection method which is characterized in that when the snow density is detected, a section of snow with a preset volume is cut from top to bottom, the snow is heated and melted, then the weight of the snow is detected, and then the snow density is obtained by dividing the weight by the volume. The method has the advantage that the snow density parameter can be detected more conveniently, quickly and accurately.

The invention provides a method for detecting basic lanthanum carbonate impurities in a lanthanum carbonate raw material or a preparation thereof in a solid state. The method comprises the following steps: fully grinding lanthanum carbonate or a preparation thereof, adding different amounts of basic lanthanum carbonate, fully and uniformly mixing the mixture with corundum according to a certain ratio to prepare a series of standard curve samples, scanning each standard curve sample by using a powder X-ray diffraction method, and drawing a standard curve to obtain a linear equation; fully and uniformly mixing to-be-detected lanthanum carbonate or a preparation thereof with corundum according to a certain ratio to prepare a test sample; carrying out measurement based on the same way, substituting a ratio of the diffraction intensities of the characteristic peaks of the basic lanthanum carbonate and corundum in the test sample into a linear equation to calculate the content of basic lanthanum carbonate in the sample. The method can be used for analyzing and detecting basic lanthanum carbonate impurities in lanthanum carbonate or lanthanum carbonate preparations. The method has advantages of simple operation, wide linear range, good linear relation, high precision, high accuracy, high durability and high detection sensitivity.