Gastric cancer tumor marker and application thereof

A tumor marker, gastric cancer technology, applied to gastric cancer tumor markers and their application fields, can solve the problems of low early diagnosis rate of gastric cancer, and achieve simple and accurate diagnosis of gastric cancer, simple, rapid and accurate detection, and is suitable for large-scale promotion and application. Effect

Pending Publication Date: 2020-11-06
上海尤里卡信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The vast majority of gastric cancer is adenocarcinoma. There are no obvious symptoms in the early stage, or non-specific symptoms such as epigastric discomfort and belching, which are often similar to the symptoms of chronic gastric diseases such as gastritis and gastric ulcer, and are easily ignored. Therefore, the early diagnosis of gastric cancer in my country is currently rate is still low

Method used

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  • Gastric cancer tumor marker and application thereof
  • Gastric cancer tumor marker and application thereof
  • Gastric cancer tumor marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Quantitative RT-PCR detection of AGR3 gene expression in gastric cancer tissue

[0041] 1. Tissue separation: The experimental tissue was derived from surgical patients with primary gastric cancer. Once the surgically resected stomach was isolated, the lesion and surrounding paracancerous tissues 5 cm away were quickly excised and stored in liquid nitrogen (-80°C). The diagnosis of cancer and paracancer is based on pathological diagnosis.

[0042]2. Extraction of ribonucleic acid (RNA): total RNA was extracted with a Trizol kit (Invitrogen), and the extraction method was referred to the instructions of the Trizol kit. When RNA is ready to be used, the RNA can be precipitated using the following method: add NaAc to 0.3M, centrifuge at 12,000×g for 5 minutes.

[0043] 3. Synthesis of cDNA:

[0044] (1) Add 4 μL of template RNA (0.5 μg / μL), 2 μL of 20 pmol / μL random primer, 5 μL of deionized water into the ice bath centrifuge tube, mix well, and centrifuge for...

Embodiment 2

[0056] Example 2 Quantitative RT-PCR detection of AGR3 gene expression in gastric cancer cell lines

[0057] The expression of AGR3 gene in human gastric epithelial cell line GES-1 and human gastric cancer cell lines BGC823, HGC27, AGS, NCI-N87 and SNU-1 was detected by quantitative RT-PCR. Wherein said cell line comes from Shanghai Cell Bank of Chinese Academy of Sciences.

[0058] According to the method described in Example 1, RNA was extracted from different gastric cancer cell lines, and cDNA was synthesized by reverse transcription. Using GAPDH as an internal control, and using the synthesized cDNA of different gastric cancer cell lines as a template, the PCR reaction was carried out according to the method described in Example 1. Agarose PCR amplification products were detected by gel electrophoresis, and the results were shown in figure 2 .

[0059] Depend on figure 2 The results showed that AGR3 gene was highly expressed in AGS, NCI-N87 and SNU-1 cells, but only ...

Embodiment 3

[0060] Example 3 Expression of AGR3 and changes in cell proliferation ability after knockdown and overexpression of AGR3 in gastric cancer cell lines AGS and NCI-N87

[0061] In vitro chemical synthesis of short hairpin ribonucleic acid (shRNA) has the characteristics of rapidity, simplicity and strong specificity, and has broad application prospects in anti-tumor therapy and other aspects. Using shRNA to study the effect of AGR3 gene on the growth and proliferation of AGS and NCI-N87 cells.

[0062] Two shRNA sequences were designed and synthesized for the AGR3 gene mRNA, respectively AGR3 (s1) and AGR3 (s2), respectively the nucleotide sequence shown in SEQ ID NO:5 and the nucleotide sequence shown in SEQ ID NO:6 ; An irrelevant sequence NC is set as a negative control, such as the nucleotide sequence shown in SEQ ID NO: 9; in addition, the full sequence of the AGR3 gene is chemically synthesized in vitro, as shown in SEQ ID NO: 1; an empty vector (vector) is set as a negati...

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Abstract

The invention provides a gastric cancer tumor marker. An amino acid sequence of the gastric cancer tumor marker is as shown in SEQ ID NO:2. Preferably, a nucleotide sequence of the gastric cancer tumor marker is as shown in SEQ ID NO:1. The invention further provides an application of a pair of gastric cancer tumor markers. The invention further provides a detection primer pair of the gastric cancer tumor marker and an application of the detection primer pair. The invention further provides an inhibitor of the gastric cancer tumor marker and an application of the inhibitor. The gastric cancertumor marker can enable gastric cancer diagnosis to be simple, accurate and rapid, improves the gastric cancer early diagnosis level, and is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, more specifically, to the technical field of tumor markers, in particular to a gastric cancer tumor marker and its application. Background technique [0002] Gastric cancer (gastric carcinoma) is a malignant tumor originating from the gastric mucosal epithelium. It ranks first in the incidence of various malignant tumors in my country. The incidence of gastric cancer has obvious regional differences. The incidence of gastric cancer in the northwest and eastern coastal areas of my country is higher than that in the south. region is significantly higher. The age of onset is more than 50 years old, and the ratio of male to female incidence is 2:1. Due to changes in diet structure, increased work pressure, and Helicobacter pylori infection, gastric cancer tends to be younger. Gastric cancer can occur in any part of the stomach, and more than half of them occur in the gastric antrum. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/90C12N15/61G01N33/574G01N33/573C12Q1/6886C12N15/11A61K31/7088A61P35/00
CPCC12N9/90G01N33/57446G01N33/57484G01N33/573C12Q1/6886A61K31/7088A61P35/00C12Y503/04001G01N2333/99C12Q2600/158
Inventor 梁翠霞谈竹君
Owner 上海尤里卡信息科技有限公司
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