Rapid quantitative detection method of cocaine suitable for field detection

A quantitative detection method and on-site detection technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of lack of rapid quantitative detection methods for cocaine, improve fluorescence quenching efficiency, improve analysis and detection sensitivity, and reduce background signals Effect

Active Publication Date: 2016-06-15
GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, there is still a lack of satisfactory cocaine rapid quantitative detection methods applicable to on-site detection in this area. Theref

Method used

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  • Rapid quantitative detection method of cocaine suitable for field detection
  • Rapid quantitative detection method of cocaine suitable for field detection
  • Rapid quantitative detection method of cocaine suitable for field detection

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Embodiment 1

[0049] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:

[0050] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 3 mm, and 6 μL of a mixed solution of PEI-UCPs and ACA-1 is added to each hydrophilic detection area , wherein the concentration of the water-soluble up-conversion fluorescent nanomaterial PEI-UCPs is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 2 μM;

[0051] (2) Prepare AuNPs-ACA-2 solution as described above;

[0052] (3) Concentration optimization of AuNPs-ACA-2 solution: Add 3 μL of 10 μM cocaine solution and 3 μL of AuNPs-ACA-2 solution of different concentrations to the hydrophilic detection area of ​​the paper-based detection device described in step (1) respectively (concentration 0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315μM), rea...

Embodiment 2

[0063] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:

[0064] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 2 mm, and 2.6 μL of a mixture of PEI-UCPs and ACA-1 is added to each hydrophilic detection area Solution, wherein the concentration of water-soluble up-conversion fluorescent nanomaterial is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 0.5 μM;

[0065] (2) Prepare AuNPs-ACA-2 solution as described above;

[0066] (3) Concentration optimization of AuNPs-ACA-2 solution: add 1.3 μL of 10 μM cocaine solution and 1.3 μL of AuNPs-ACA-2 solution of different concentrations to the hydrophilic detection area of ​​the paper-based detection device described in step (1) (Concentrations were 0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315 μM), reacted at 20...

Embodiment 3

[0072] A cocaine rapid quantitative detection method suitable for on-site detection, comprising the following steps:

[0073] (1) Prepare a paper-based detection device as described above, wherein the paper is made of cellulose filter paper, and the inner diameter of the circular hydrophilic detection area is 6 mm, and 24 μL of a mixed solution of PEI-UCPs and ACA-1 is added to each hydrophilic detection area , wherein the concentration of water-soluble up-converting fluorescent nanomaterials is 0.5 mg / mL and the concentration of single-stranded nucleic acid ACA-1 is 4 μM;

[0074] (2) Prepare AuNPs-ACA-2 solution as described above;

[0075] (3) Concentration optimization of AuNPs-ACA-2 solution: Add 12 μL of 10 μM cocaine solution and 12 μL of AuNPs-ACA-2 solution of different concentrations to the hydrophilic detection area of ​​the paper-based detection device described in step (1) respectively (concentration 0, 0.07, 0.105, 0.14, 0.175, 0.21, 0.28, 0.315μM), reacted at 3...

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Abstract

The invention discloses a rapid quantitative detection method of cocaine suitable for field detection, comprising the following steps: (1), preparing a paper base detector: using paper as a detection base, outlining a hydrophilic detection area on the surface of the paper by using a hydrophobic barrier, and covalently coupling water-soluble up-conversion fluorescent nano-material and aptamer ACA-1; (2), surface modifying gold nanoparticles with aptamer ACA-2 to obtain solution AuNPs-ACA-2; (3), deeply optimizing the solution AuNPs-ACA-2; (4), preparing a standard solution of cocaine; (5), drawing a standard curve for cocaine detection; (6) measuring cocaine concentration in bodily fluid to be detected.The unsolved problem with rapid quantitative detection in the field of cocaine detection is solved, rapid, quantitative and highly sensitive detection of cocaine concentration in a bodily fluid sample is implemented, and quantitative detection sensitivity is up to 0.05 MuMu.

Description

technical field [0001] The invention belongs to the technical field of rapid quantitative detection of cocaine, in particular to a paper-based detection method for rapid, quantitative and highly sensitive detection of cocaine based on up-conversion fluorescent nanometer materials. Background technique [0002] Drug abuse is one of the most serious social problems that endanger mankind at present, and cocaine is the most widely used one among all kinds of drugs. Cocaine abuse will pose a great threat to the human body, causing human heart rate to increase, shortness of breath, and even tremors, convulsions, convulsions and other phenomena, posing a huge threat to human security, social stability and world peace. Therefore, the study of new, sensitive, and quantitative cocaine detection methods has very important practical significance and application value for drug control, poison detection, and criminal identification. [0003] The traditional cocaine detection methods that...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/643G01N2021/6432
Inventor 刘志洪何梦媛朱勇王诗诗李贞葛依颖
Owner GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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