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Florfenicol quick detection kit and florfenicol quick detection method

A detection kit, florfenicol technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex pre-treatment, time-consuming, etc., to achieve the effect of reducing matrix effect, good sensitivity and specificity

Inactive Publication Date: 2020-05-12
CUSABIO TECH LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have high sensitivity and good reproducibility. Among them, the national standard method is liquid chromatography-mass spectrometry, but the pretreatment of samples is complicated and time-consuming, requiring professionals and specific instruments and equipment

Method used

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  • Florfenicol quick detection kit and florfenicol quick detection method
  • Florfenicol quick detection kit and florfenicol quick detection method
  • Florfenicol quick detection kit and florfenicol quick detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment one colloidal gold detection card

[0018] 1. Preparation of colloidal gold solution (trisodium citrate reduction method): Take 100 mL of 0.01% chloroauric acid aqueous solution, heat it to boiling with a constant temperature electromagnetic stirrer, add 2.5 mL of 1% trisodium citrate aqueous solution while stirring continuously, Continue stirring and heating for 10 min, the solution turns bright red. Cool at room temperature, restore to the original volume with deionized water, and store at 4°C for later use.

[0019] 2. Labeling of monoclonal antibodies: Take 100 mL of the prepared colloidal gold solution and adjust the pH to 8.0 with 0.1 mol / L potassium carbonate solution. While stirring, 2 mg of mouse anti-florfenicol was added, stirred for 15 min, and 2.5 mL of 25 mg / mL polyethylene glycol 20 000 (PEG 20 000) was added dropwise, and stirred for 20 min. Centrifuge at 20 000 r / min for 20 min, discard the supernatant, add 10 mL pH7.4 PB...

Embodiment 2

[0022] Example 2. The kit detects egg samples

[0023] Weigh 1g of the egg sample that was stirred evenly into a 10mL centrifuge tube, add 2mL of the sample extract, vortex for 1min, take 4 drops of the mixed solution, and add it to the sample hole of the Florfenicol test card for detection.

[0024] Take out the test card, put it flat on the table after opening, moisten the sample pad of the test card with the sample extraction solution before testing, then use the dropper attached to this product to absorb the test solution, add 4 drops to the sample hole; add the sample After 5 to 8 minutes, observe the color-developed area and judge the result. For the judged result, the concentration of florfenicol in the sample is further judged by the analytical instrument.

[0025] Result Judgment Principles:

[0026] Negative: The C line is colored, and the T line is visible to the naked eye, regardless of the depth of the color, it is judged as negative.

[0027] Positive: The C li...

experiment example 1

[0031] Experimental example 1 Analytical performance of colloidal gold immunochromatographic test strip

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Abstract

According to a florfenicol quick detection kit, the colloidal gold is taken as a marker. The competitive inhibition principle is adopted, so that a high-sensitivity florfenicol colloidal gold immunochromatography quantitative detection method is established. According to the invention, a colloidal gold immunochromatography technology is utilized; a nitrocellulose membrane is coated with a florfenicol antigen (test line) and goat anti-mouse IgG (quality control line), a labeled florfenicol gold-labeled antibody is sprayed on a gold-labeled pad, when a treated egg sample flows through an NC membrane, a red line is displayed on the NC membrane, and whether the sample contains florfenicol or not is judged according to whether a detection line (T line) exists or not.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a florfenicol detection kit and a detection method. Background technique [0002] Florfenicol (FF), also known as florfenicol, is a new veterinary-specific broad-spectrum antibacterial drug developed by Schering-Plough in 1988. It is widely used in aquatic products. and livestock and poultry farming. Studies have shown that florfenicol has hematotoxicity, embryotoxicity and immunosuppressive effects, and adverse reactions such as transient anorexia and diarrhea may occur after administration to animals. [0003] In the breeding process, the excessive use of florfenicol will cause its residue in animal food, which will pose a potential threat to the health of consumers and lead to the emergence of drug-resistant bacteria. Therefore, countries have successively stipulated the maximum residue limit standard of florfenicol in animal food. Florfenicol has certain immunotoxicity and embr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/558G01N33/577
Inventor 周陶鸿李诗瑶刘艳华权高余箭翔夏欣闫彩霞
Owner CUSABIO TECH LLC
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