Kit for determining antianxiety/hypnotic type drugs in serum and plasma through liquid chromatography tandem mass spectrometry and application thereof

Abstract

The invention provides a kit for determining antianxiety/hypnotic type drugs in serum and plasma through liquid chromatography tandem mass spectrometry. The kit comprises the following constituents: drug standards comprising bromazepam, clonazepam, diazepam, lorazepam, midazolam, nitrazepam, oxazepam and Temazepam; drug internal standard compounds comprising alprazolam-d5, clonazepam-d4, diazepam-d5, lorazepam-d4, midazolam-d4, nitrazepam-d5, oxazepam-d5 and Temazepam-d5; drug extraction compositions comprising, by volume, 60% of methanol solution, 20% of acetonitrile solution, 10% of isopropanol solution and 10% of purified water; negative plasma; and diluent: 50% of carbinol water solution. The kit can be used for simultaneously determining antianxiety/hypnotic type drugs and active metabolites thereof, and has the advantages of short determination time and high flux.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular to a kit for detecting anxiolytic / hypnotic drugs in serum plasma by liquid chromatography tandem mass spectrometry and its application. The kit is suitable for detecting anxiolytics in serum plasma by tandem mass spectrometry / Hypnotic drugs and their metabolites. Background technique [0002] Anti-anxiety / hypnotic drugs are good medicines that effectively help sleep and improve sleep. They can avoid the serious harm of insomnia to the human body. They are used to treat insomnia and improve sleep quality. They are mainly used for symptomatic treatment of anxiety and irritability. Common anti-anxiety / hypnotic drugs include phenobarbital, meproton, chlordiazepoxide, and diazepam. However, these drugs have certain side effects: 1. Nausea or vomiting; 2. General malaise or fatigue; 3. Tachycardia 4. Anxiety or agitation; 5. Decreased postural blood pressure; 6. Coarse tremors ...

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Problems solved by technology

[0004] Sedatives also belong to the category of anti-anxiety drugs. Currently on the market, a journal published in "Chromatography" in 2010 "Ultra-high performance liquid chromatography-tandem mass spectrometry detects 10 kinds of sedative drugs remaining in pork and pig kidneys" , although the target objects of its detection are pork and pig kidney, it is still not directly detecting sedative drugs and their metabolites in human serum and / or plasma

Similarly, although there are other literatures that also disclose methods for detecting anxiolytic drugs by liquid chromatography tandem mass spectrometry, none of them directly detect anxiolytic drugs and their metabolites in human serum and / or plasma.

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