126 results about "Drug extraction" patented technology

The invention discloses a medicinal and edible medicine extract, a medicinal composition and a preparation method thereof, and belongs to the technical field of medicinal and edible traditional Chinese medicines. The medicine extract comprises the following raw materials: hovenia dulcis thunb, lalang grass rhizome, lophatherum gracile, corn stigma, emblic leafflower fruit, medlar and pericarpium citri reticulatae. In addition, the invention also provides a preparation method of the medicine extract, which comprises the following steps: mixing the raw materials in proportion, adding 5-6 times of water, decocting twice, filtering and combining filtrate, and concentrating under reduced pressure until the relative density is 1.1-1.2 to obtain the medicine extract. In addition, the invention further provides a pharmaceutical composition which comprises the pharmaceutical extract, fructo-oligosaccharide liquid, corn oligopeptide powder, a freeze-dried complex microbial inoculant, oyster peptide powder and DL-malic acid. The medicinal extract provided by the invention has the advantages that the liver protection effect is also improved while the alcoholism relieving effect is improved.

The utility model discloses a multi-station perfluorohexanone quantitative filling system which comprises a filling operation table, a filling station area, a nitrogen drying device and a medicament extraction assembly. The utility model further discloses a multi-station perfluorohexanone quantitative filling method. The multi-station perfluorohexanone quantitative filling method comprises the following steps that S1, an inspection stage before filling is conducted; s2, a nitrogen replacement stage; s3, a medicament filling stage; s4, a nitrogen filling stage; and S5, a filling completion stage. According to the device, the filling process can be automatically completed through PLC control; a connector of the filling steel cylinder is a quick-release connector, and after filling is completed, the filling steel cylinder can be quickly replaced for next filling; according to the device, the water content in the perfluorohexanone filling process is controlled in two aspects, the overall function is complete, and the practicability is high.

The invention provides a method for extracting acacetin-7-O-glucuronide from dracocephalum moldavica, and relates to the technical field of medicine extraction. The method comprises the following steps: heating and extracting dracocephalum moldavica or dracocephalum moldavica products by adopting an alkaline solution, and precipitating the obtained extracting solution under the condition that the pH value is 1-3 to obtain a precipitate extract; mixing n-hexane, ethyl acetate, ethanol and water, and layering to obtain an upper phase solution and a lower phase solution; and dissolving the precipitate extract with a lower-phase solution, carrying out high-speed counter-current chromatography separation on the obtained sample solution, and collecting an acacetin-7-O-glucuronide fraction according to a chromatogram. According to the method, alkali extraction, acid precipitation and high-speed counter-current chromatography are combined, process conditions are optimized, the high-purity monomer of acacetin-7-O-glucuronide in dracocephalum moldavica is rapidly prepared, and the process is simple.

The invention relates to the technical field of medicine extraction, in particular to a method for extracting ginseng essential oil through combination of a deep eutectic solvent-supercritical technology and column chromatography. The method comprises the following steps: (1) crushing ginseng root hair, adding the crushed ginseng root hair into a supercritical extraction kettle, and carrying out supercritical extraction under the combined action of liquid CO2 and an entrainer to obtain an extract; the entrainer comprises absolute ethyl alcohol and a eutectic solvent; and (2) concentrating the extract until no ethanol exists, diluting with water, adsorbing with macroporous resin, carrying out column chromatography, washing with water, eluting with 2%-5% ethanol, eluting with 10%-20% ethanol, collecting 10%-20% ethanol eluent, and drying to obtain the product. The method has the advantages of high yield, high ginseng alkynol content, short extraction period and no environmental pollution.

The invention relates to the technical field of medicine extraction, and discloses a vacuum low-temperature distillation herbal medicine bath fine extraction process, which comprises the following steps: 1, pretreating raw materials; step 2, crushing and mixing; step 3, vacuum low-temperature distillation; step 4, filtering treatment; step 5, concentrating and drying; and step 6, carrying out post-treatment on the extract. According to the vacuum low-temperature distillation herbal medicine bath fine extraction process, the vacuum low-temperature distillation condition is accurately controlled, so that the heat-sensitive component retention rate is greatly increased, and the medicated bath effect is enhanced; in the aspect of impurity removal, various filtering and chromatography technologies are cooperated, so that the impurity content is greatly reduced, and the use safety is guaranteed; in terms of energy consumption, low-temperature and low-pressure operation achieves great energy conservation, and large-scale production is facilitated; after the extraction process is optimized, by means of microwave, superfine grinding, ultrasonic mixing and the like, the utilization rate of the herbal medicine is remarkably increased, high-quality, high-efficiency and high-safety innovation breakthrough is brought to the herbal medicine medicated bath industry, and the method has great market competitiveness and application and popularization value.

The invention belongs to the technical field of medicine extraction, and discloses a preparation method and application of a mangnolia officinalis extract. The preparation method is characterized by comprising the following steps: adding a weak base reagent into officinal magnolia bark coarse powder, freezing, and grinding; and adding a mixed solution of ethanol and ethyl acetate for ultrasonic extraction, carrying out solid-liquid separation, collecting a separation liquid, and removing a solvent from the separation liquid to prepare the magnolia officinalis extract. By adding the weak base reagent and freezing treatment, the damage to active substances in the mangnolia officinalis powder in the grinding process can be effectively reduced, the mangnolia officinalis raw material can be ground to be small in particle size, and the active components are not affected. And then the mangnolia officinalis powder with smaller particle size is extracted from the mangnolia officinalis by adopting a mixed solution of ethanol and ethyl acetate, and the proportion of the two solvents is controlled, so that the antibacterial effect and the oxidation resistance of the mangnolia officinalis extract can be remarkably improved.

The utility model discloses an insect repellent device for kiwifruit planting, comprising an unmanned aerial vehicle (UAV) body, a medicine storage box being arranged below the UAV body, a spraying mechanism being arranged on the UAV body, the spraying mechanism comprising a supporting frame, a connecting piece, a conveying pipe, a nozzle and a water pump, the supporting frame being mounted on the UAV body, the connecting piece being provided with a plurality of groups mounted on the supporting frame, the conveying pipe being mounted on the connecting piece, the nozzle being mounted on the bottom end of the connecting piece, the water pump being mounted on one side of the connecting piece, a medicine extraction mechanism being arranged below the medicine storage box, the medicine extraction mechanism comprising a vacuum pump, a fixed pipe, a connecting pipe, a threaded pipe and a medicine extraction head, the vacuum pump being mounted on the bottom surface of the medicine storage box, so as to solve the technical problems mentioned in the background technology that the spraying of the insect repellent is uneven, errors are likely to occur when the insect repellent is added manually, and the mixing of the insect repellent and water in the medicine storage box is uneven.

The invention relates to the field of flowing medicine discharging control and detection, in particular to a control and detection method applied to a flowing medicine discharging device. A specific embodiment of the method comprises the following steps: controlling a mobile medicine extraction device corresponding to a mobile medicine type to extract mobile medicines from a corresponding mobile medicine library; in response to determination of completion of extraction of the flowable medicine, controlling the flowable medicine extraction device to input the extracted flowable medicine into the liquid medicine storage test tube, and controlling the image acquisition device to acquire a medicine outflow video at a medicine outlet tube of the flowable medicine extraction device in real time, a medicine outlet pipe of the mobile medicine extraction device is transparent, and a liquid medicine storage test tube is transparent; and determining whether the input of the flowable drug is completed, and generating drug configuration completion information in response to determining that the input of the flowable drug is completed. According to the implementation mode, the accuracy and efficiency of liquid medicine discharging configuration are improved, and the liquid medicine extraction efficiency and safety are improved.

The invention discloses a medicine extraction system and method based on a molecular biological technology and chromatographic equipment, and belongs to the technical field of pharmaceutical separation and purification. The medicine extraction system based on the molecular biological technology comprises a medicine extraction module, the medicine extraction module comprises a chromatographic column, a constant flow pump, a sample injector and a collector, the output end of the constant flow pump is connected with an inlet of the chromatographic column, the sample injector is connected with the chromatographic column through a valve, and the collector receives eluent output by the chromatographic column; the operation module is used for acquiring physical and chemical data of the target product, determining first operation data of the chromatographic equipment according to the physical and chemical data, and generating a first matching vector according to the physical and chemical data and the first operation data; predicting a prediction extraction result of the target product according to the first matching vector and a preset clustering recognition space; and the control module is used for controlling the constant flow pump according to the first operation data and the prediction extraction result, and the production efficiency is improved by reducing the test verification work.

The invention discloses a pretreatment method and application of a mirabegron resin compound, and relates to the pretreatment method of the mirabegron resin compound, which comprises the following steps: extracting the mirabegron resin compound by an extraction solvent to obtain a sample to be detected for detection; wherein the extraction solvent comprises an aprotic solvent, an ionized solvent and a counter-ion solvent. According to the method, a multi-factor synergistic strategy is adopted, the extraction time of mirabegron in the mirabegron resin compound is remarkably shortened, the medicine extraction efficiency is remarkably improved, the extraction method is high in recovery rate, meanwhile, comprehensive optimization of quality is ensured, a foundation is laid for accurate determination of the medicine content, the consistency of the medicine effect is ensured, and the method is suitable for large-scale popularization and application. The requirements of links such as drug research and development, production and quality control are met.

The invention provides a preparation method of a dogwood extract and application of the dogwood extract in bone strengthening products, and belongs to the technical field of medicine extraction. The method comprises the following steps: (1) crushing a dogwood raw material, and immediately spraying an acid solution; (2) sequentially extracting the pretreated material by adopting an ethanol water solution containing citric acid and L-ascorbic acid as a first-phase extraction solvent and a phosphate buffer solution containing a compound enzyme preparation as a second-phase extraction solvent; (3) combining the extracting solutions, sequentially separating through an ultrafiltration membrane and at least two stages of nanofiltration membranes to obtain active fractions with different molecular weight intervals, and recombining the active fractions according to a preset proportion to obtain a refined solution; and (4) drying the refined liquid to obtain the dogwood extract. The preparation method provided by the invention has the advantages of high extraction rate of target active ingredients (especially aglycone), synergistic retention of multiple active ingredients, strong process controllability, suitability for large-scale production and the like.

The present invention belongs to the field of probe technology, and provides a trifunctional probe based on a photocrosslinking group, a preparation method thereof, and an application thereof. The method comprises the following steps: under a protective atmosphere, compound 1, compound 2, 1-hydroxybenzotriazole, dimethylformamide, carbodiimide, and N,N-diisopropylethylamine are mixed, and a condensation reaction is performed to obtain a trifunctional probe based on a photocrosslinking group. The probe provided by the present invention can be bonded with baicalein, TFAE, and scutellaria baicalensis medicated serum to form a corresponding probe, and compete with its original compound. Under excitation of different ultraviolet wavelengths, the probe can be cross-linked with small molecules and target proteins in traditional Chinese medicine, and accurately reflect the level of target protein labeling of the reactive group in the proteome through a "reporter group", and can also selectively enrich the labeled target protein, and achieve the identification of single compounds, compound combinations, and natural drug extract targets through subsequent experiments.

The invention discloses a traditional Chinese medicine preparation with a liver protection effect and a preparation method and application thereof, and belongs to the technical field of application of medicine extracts. The traditional Chinese medicine preparation is prepared from a gallnut extract, an auxiliary material and an ethanol solution, the mass ratio of the gallnut extract to the auxiliary material is 1: (1-2), and the ratio of the total mass of the gallnut extract and the auxiliary material to the mass volume of the ethanol solution is 100g: (2-5) mL. The traditional Chinese medicine preparation disclosed by the invention has the advantages that the antioxidant capacity of mice is improved, the anti-inflammatory capacity of mice is enhanced, the liver injury of the mice caused by toxicants such as acetaminophen and carbon tetrachloride is relieved, and the intestinal flora structure and liver metabolism condition of the mice are improved; when the compound is used for preparing the liver protection preparation, an antioxidant signal channel of a body can be activated, and oxidation and fibrosis damage of the liver can be relieved, so that the liver function of the body is improved.

The utility model discloses an anesthetic quantitative preparation device which comprises a bearing table used for placing a mixed anesthetic receiving container, a plurality of quantitative preparation units are arranged above the bearing table through a supporting structure, and each quantitative preparation unit comprises a drug temporary storage cylinder and a piston type preparation cylinder which are arranged in parallel. The two are communicated through a medicine pumping pipeline with a first one-way valve, and the medicine pumping pipeline is further communicated with a medicine feeding pipeline with a second one-way valve; wherein a lifting structure is arranged relative to the piston of the piston type preparation cylinder, and the lifting structure is used for adjusting the stroke of the piston, so that the anesthetic can be quantitatively sucked in from the medicine pumping pipeline or quantitatively sent out from the medicine feeding pipeline. The utility model aims to provide the quantitative anesthetic preparation device which can assist an anesthetist in mixing and preparing anesthetic, realizes quantitative suction and delivery of the anesthetic by accurately controlling the stroke of the piston through the lifting structure, is low in labor intensity, and is beneficial to ensuring that the content of each component in the mixed anesthetic is accurate.

Methods and systems for detecting and tracking drug extraction from one or more drug storage apparatus at a central server. The system involves one or more authenticated drug storage apparatus and the central server. An access detection computer circuit integrates with packaged drugs, creating detectable drug packaging. The access detection computer circuit provides drug access detection trigger signals when drugs are accessed from the detectable drug packaging. There is a communication device for transmitting secure communication messages between the components.

This application discloses a sustained-release formula and medicated pillow for the prevention and treatment of allergic rhinitis, including a pillow base with two symmetrically arranged sustained-release mechanisms and a drive mechanism in the bottom mounting compartment. The sustained-release mechanism consists of multiple sustained-release components, each containing a sustained-release tube and a drug storage tube, connected by a U-shaped connecting pipe and a one-way valve; the drug storage tube is inserted into a pierced tube with a filter screen, which balances air pressure and prevents contamination. The drive mechanism drives a sliding valve to reciprocate via a worm gear system, achieving quantitative drug extraction and precise sustained-release; the atomization design of the sustained-release port improves drug absorption efficiency, and the guide holes in the mounting compartment and the ventilation grooves on the pillow surface form a uniform diffusion channel. This solution solves the problems of cumbersome drug replacement, uncontrollable volatilization, and easy deterioration associated with traditional medicated pillows, offering advantages such as precise controlled release, convenient maintenance, and multiple safety protections. It allows for individualized drug administration based on drug characteristics and patient rhythms, significantly improving the effectiveness of sleep aid therapy and the user experience.

The invention relates to the technical field of medical drugs, and particularly discloses a honeysuckle flower and mango capsule and a preparation method thereof. The honeysuckle flower and mango capsule comprises a medicine-containing core material and a plant capsule shell, the medicine-containing core material comprises the following raw materials: a medicine extraction composition, a filling agent and an adhesive. The medicine extraction composition is prepared by adopting a specific extraction method, effective components in all plants can be rapidly extracted through reflux extraction and decoction extraction, damage to the effective components is reduced, and the extraction efficiency is high; and effective components in the raw materials are extracted more thoroughly through a percolation method, so that the purity and quality of the extract are improved. The medicine-containing core material further comprises a filling agent and an adhesive besides the medicine extraction composition, the stability of effective components in the medicine extraction composition can be improved, continuous and stable release of the medicine effective components is guaranteed, and the treatment effect of the honeysuckle flower and mango capsule is improved. The plant capsule shell is selected as a hard capsule and is not easily polluted by microorganisms, so that the stability of the medicine is improved, and the medicine is convenient for patients to take.

Methods and systems for detecting and tracking drug extraction from one or more drug storage apparatus at a central server. The system involves one or more authenticated drug storage apparatus and the central server. An access detection computer circuit integrates with packaged drugs, creating detectable drug packaging. The access detection computer circuit provides drug access detection trigger signals when drugs are accessed from the detectable drug packaging. There is a communication device for transmitting secure communication messages between the components.

The invention relates to the technical field of traditional Chinese medicine extraction equipment, and discloses a medicinal material extraction workshop clean automatic feeding device which comprises a rotary medicinal material conveying mechanism. According to the clean automatic feeding device for the medicinal material extraction workshop, a screw raw material conveying principle is utilized, medicinal materials can be ground into fine particles firstly in the feeding process, then the fine particles are quantitatively discharged downwards, and therefore the effective extraction degree of the medicinal materials in the medicinal material extraction process is improved; the device has the functions of instantly opening medicinal material feeding and closing medicinal materials, so that the capability of controlling the feeding amount of the medicinal materials is improved.

The invention discloses a preparation method and application of a mulberry leaf extract, and belongs to the technical field of natural medicine extraction. The preparation method comprises the following steps: S1, crushing mulberry leaves, adding water, extracting, filtering, collecting filtrate, cooling and centrifuging the filtrate, and retaining clear liquid; s2, enabling the clear liquid to pass through macroporous adsorption resin, and collecting effluent; s3, carrying out microfiltration and concentration on the effluent, and collecting a concentrated solution; and S4, adding ethanol into the concentrated solution, standing for crystallization, and drying the crystal under reduced pressure to obtain the mulberry leaf extract. The yield of the extract prepared through the preparation method is high, the content of mulberry leaf polysaccharide in the prepared extract is high, and the mulberry leaf extract can be applied to feed for improving the body length of crucian carps, feed for improving liver health of crucian carps and feed for improving intestinal health of crucian carps.

The invention discloses an L-carnitine-based ionic liquid aqueous two-phase system as well as a preparation method and application thereof, and belongs to the technical field of aqueous two-phase system separation. The prepared L-carnitine-based ionic liquid aqueous two-phase system is a novel ionic liquid aqueous two-phase system which is more efficient when active substances are extracted (extraction and separation can be achieved by stirring for 30 min and centrifuging for 5-8 min at the speed of 6000-8000 rpm), the extraction rate of vanillin, eugenol, p-hydroxybenzaldehyde, syringaldehyde and vanillic acid reaches 85.37% or above, and the L-carnitine-based ionic liquid aqueous two-phase system is a novel ionic liquid aqueous two-phase system. The method can be applied to the fields of organic compound extraction, medicine extraction, environmental protection and the like, and has a great development prospect.

The medicine extraction equipment comprises a control box and a smashing box installed on the control box, the smashing box comprises a feeding port in the top and a discharging port in one side, the discharging port extends into an extraction box through a connecting pipe, a main discharging pipe is arranged at the bottom of the extraction box, and the main discharging pipe is connected with the control box. An inverted U-shaped siphon is arranged on one side of the extraction box, one end of the siphon extends towards the inner bottom of the extraction box, the other end of the siphon penetrates through the extraction box and extends vertically and downwards, a heating tank is further arranged on the side, away from the control box, of the extraction box, and the top of the heating tank is communicated with a steam conveying pipe; the steam conveying pipe is connected with one end of the condenser, and the other end of the condenser extends into the extraction box through a pipeline; the medicine extraction equipment and the extraction process are stable in structure, easy and convenient to operate, capable of avoiding manual intervention and impurity pollution in the extraction process, capable of improving medicine active ingredients and suitable for application and popularization.

The present invention provides a method for extracting flavonoid components in safflower, and relates to the technical field of drug extraction. The present invention mixes safflower with alkali solution for heating extraction to obtain an extract; the extract is mixed with acid, and precipitated under the condition of pH value of 1 to 3 to obtain a precipitated extract; the precipitated extract is dissolved and separated by preparative liquid chromatography to obtain Fr1 to Fr6 fractions; the Fr1 to Fr6 fractions are respectively concentrated to dryness to obtain luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D-glucuronide, diosmetin-7-O-β-D-glucuronide, cynarin, acacetin-7-O-glucuronide, and acacetin in sequence. The present invention quickly realizes the acquisition of high-purity monomers of the main flavonoid components in safflower, and the purity of each component is 98 to 99%.

The invention discloses a herbal medicine extraction and concentration device, and relates to the technical field of herbal medicine extraction and concentration, the herbal medicine extraction and concentration device comprises a tank body and a feeding pipe, the feeding pipe is fixedly communicated with the top of the tank body, the top end of the tank body is provided with a tank cover, the tank cover is rotatably connected with a turntable, the top surface of the turntable is fixedly connected with a belt wheel, and the top surface of the tank cover is fixedly connected with a servo motor I; a belt is in transmission connection between an output shaft of the first servo motor and the belt wheel, a top supporting plate is fixedly connected to the top face of the tank cover, and a second servo motor is fixedly connected to the side, close to the belt wheel, of the top supporting plate. Impurities in an extracting solution are filtered and separated in real time through a filter disc, the impurities are prevented from entering a concentration link, extraction and concentration are carried out at the first time after filtering and next to an extraction and concentration step, crystals or precipitates are prevented from being generated again in a solution waiting period, and the impurities are prevented from changing physical properties of the solution; insoluble impurities are prevented from being attached to the heating surface to form a heat insulation layer.

The invention discloses a phoebe zhennan water-soluble extract as well as an extraction method and application thereof, and belongs to the technical field of medicine extraction. The method comprises the following steps: S1, crushing a phoebe zhennan raw material, adding 6 times of water, extracting twice, 1 hour each time, and combining filtrates; s2, concentrating the filtrate obtained in the step S1 under reduced pressure until the volume is 1: 1, standing at low temperature, and taking supernate; and S3, centrifuging the supernate obtained in S2, discarding the precipitate, concentrating under reduced pressure, and drying at low temperature to obtain the phoebe zhennan water-soluble extract. The invention discloses a phoebe zhennan water-soluble extract and an extraction method and application thereof. The phoebe zhennan water-soluble extract has efficient broad-spectrum antiviral activity and can effectively inhibit respiratory virus activity.

The invention relates to a monomolecular micelle aqueous solution as well as a preparation method and application thereof, and belongs to the technical field of biomedicine. The monomolecular micelle aqueous solution is formed by assembling an amphiphilic polyether ester multi-block copolymer in an aqueous phase. The preparation method comprises the following steps: dissolving an amphipathic multi-block copolymer in a good solvent, slowly adding deionized water into the amphipathic multi-block copolymer, or slowly dropwise adding the amphipathic multi-block copolymer into a large amount of deionized water, self-assembling to form a monomolecular micelle, and removing the good solvent in water through dialysis to obtain a monomolecular micelle aqueous solution. And the amphiphilic polyether ester multi-block copolymer has good biocompatibility and degradability. The prepared monomolecular micelle has excellent stability and cannot be dissociated along with the dilution of the polymer concentration, and the problem that the current common micelle is dissociated along with the dilution of the polymer concentration is solved. The prepared monomolecular micelle has a good solubilizing effect on water-insoluble drugs, and can be used in the fields of drug extraction, drug delivery, controllable release and the like.

The application discloses a kind of based on HPLC Jian'erle granule multi-component fingerprint construction and active evaluation method, belong to the technical field of drug analysis.The application is based on ultra-high performance liquid chromatography technology, by optimizing drug extraction and chromatographic detection method, provide the fingerprint construction method of analysis Jian'erle granule drug quality;The method comprises the steps of ultrasonic dissolution Jian'erle granule, with 0.1% phosphoric acid and acetonitrile as mobile phase for liquid chromatography detection.According to the construction method provided by the application, a fingerprint consisting of 12 characteristic peaks is obtained, of which 10 characteristic peaks are identified to compounds.The results of the methodological experiment show that the construction method provided by the application has a similarity of >0.999 between 10 batches of Jian'erle granules, and has outstanding specificity, precision, repeatability and stability.The application provides a new strategy for efficient quality detection and quality control of Jian'erle granules, and has a broad application prospect.

The extraction device comprises a grinding groove, a top cover is arranged above the grinding groove, the top cover is fixed through a buckle assembly, a feeding port is formed in the surface of the top cover, a grinding assembly is arranged on the inner side of the grinding groove, and an extraction box is arranged below the grinding assembly. When a user uses the device, through the arrangement of a corresponding structure, the whole device can complete extraction work, the device is smaller and more exquisite and convenient to carry, the top cover and the extraction box can be taken down, the inner side can be conveniently cleaned, meanwhile, the extraction box can contain extraction liquid through a liquid inlet, and the extraction efficiency is improved. And the whole extraction process is cleaner.

The invention provides an extraction method of an asiaticoside compound, and relates to the technical field of medicine extraction. The method comprises the following steps: mixing a crude centella asiatica product with a first solvent (ethanol or methanol) for extraction, concentrating an extracting solution to be dry, dissolving an obtained first concentrate with a second solvent (n-amyl alcohol or isoamyl alcohol saturated aqueous solution), mixing with a third solvent (water saturated n-amyl alcohol or isoamyl alcohol) for extraction, collecting an organic layer, and concentrating to be dry, so as to obtain the centella asiatica extract. And dissolving the obtained second concentrate with a fourth solvent (methanol or ethanol), mixing the dissolved second concentrate with a fifth solvent (dichloromethane or acetonitrile), and carrying out precipitation to obtain a precipitate which is an asiaticoside compound product. Compared with a column chromatography method, the method for extracting the asiaticoside compound by adopting a solvent method has the advantages of simplicity in operation, large sample treatment capacity, easiness in solvent recovery and application and the like, is suitable for industrial production, and can be used for extracting the asiaticoside compound with high content (not less than 70wt%) from a crude product of centella asiatica.

The present invention discloses a method for extracting the effective components of antlers by combining deep eutectic multiple extractions, belonging to the technical field of drug extraction. This method sequentially performs antler pretreatment, deep eutectic solvent preparation, deep eutectic extraction, alcohol extraction, water extraction and enzymatic hydrolysis to extract the effective components of antlers. The present invention utilizes choline chloride and glycerol in a molar ratio of 1:3 to prepare a deep eutectic solvent, optimizes the comprehensive extraction process, and obtains a protein content of 0.230~0.310 mg / kg, an amino acid content of 325~350.23 mg / kg, a polysaccharide yield of 9.0~11.61%, and a total extract yield of 20.99%. The present invention is non-toxic and pollution-free, has high extraction efficiency, simple extraction operation, and is more environmentally friendly for component extraction; at the same time, the extraction process and detection technology are optimized to shorten the detection time and make the detection results more accurate.