Method for extracting ginseng essential oil through combination of eutectic solvent-supercritical technology and column chromatography

Abstract

The invention relates to the technical field of medicine extraction, in particular to a method for extracting ginseng essential oil through combination of a deep eutectic solvent-supercritical technology and column chromatography. The method comprises the following steps: (1) crushing ginseng root hair, adding the crushed ginseng root hair into a supercritical extraction kettle, and carrying out supercritical extraction under the combined action of liquid CO2 and an entrainer to obtain an extract; the entrainer comprises absolute ethyl alcohol and a eutectic solvent; and (2) concentrating the extract until no ethanol exists, diluting with water, adsorbing with macroporous resin, carrying out column chromatography, washing with water, eluting with 2%-5% ethanol, eluting with 10%-20% ethanol, collecting 10%-20% ethanol eluent, and drying to obtain the product. The method has the advantages of high yield, high ginseng alkynol content, short extraction period and no environmental pollution.

Description

Technical Field

[0001] The invention relates to the technical field of drug extraction, and in particular to a method for extracting ginseng essential oil by combining a deep eutectic solvent-supercritical technology with column chromatography. Background Art

[0002] Ginseng essential oil, also known as ginseng volatile oil, is a mixture of volatile substances extracted from ginseng rhizomes, with the special aroma of ginseng. Studies have shown that ginseng essential oil has a wide range of biological activities such as anti-inflammatory, antitussive, anti-fatigue, hypolipidemic, alcohol and intoxication prevention, central nervous system excitation and tumor inhibition. Ginseng essential oil contains complex ingredients and many types. The main chemical components include terpenes, alcohols, ketones, aldehydes, phenols, heterocyclic and alkane compounds. Among them, the research on polyacetylene alcohol substances represented by panaxynol is more in-depth.

[0003] Panaxynol, also known as falcarinol, has a chemical name of (9 Z )-1,9-diene-4,6-diyne-heptadecan-3-ol, molecular formula C 17 H 24 O, molecular weight 244.38. The structural formula of panaxynol is as follows: .

[0004] Panaxynol is a low-polar polyacetylene component, mainly distributed in plants such as Araliaceae, Umbelliferae, and Asteraceae. Studies have shown that panaxynol has a variety of pharmacological effects, including anticancer, anticoagulation, inhibition of prostaglandin degradation, antibacterial, blood pressure reduction, sedation, analgesia, prevention of atherosclerosis, neuronal cell protection, and anti-aging. Polyacetylene components represented by panaxynol and ginseng epoxy alkynol are the most abundant in ginseng, accounting for more than 90% of the total polyacetylene content.

[0005] Due to the presence of double bonds and triple bonds in the structure of polyacetylene compounds, they are unstable when exposed to light or heat. Different extraction and separation methods directly affect the yield of polyacetylene components, and thus affect the pharmacological activity of ginseng essential oil.

[0006] The traditional extraction methods of ginseng essential oil include steam distillation, solvent extraction, supercritical CO2 extraction, etc., as follows: 1. Steam distillation After the raw material powder is soaked in water in the distiller, it is directly heated and distilled, or the raw material is placed on a porous interlayer plate net. When the steam generated by the heated water at the bottom passes through the raw material, the volatile oil is heated and distilled out at the same time as the water vapor. The distillate is collected and the oil layer is separated after cooling. However, the raw material is easily coked by strong heat, which may change the composition and the volatile oil tastes bad, reducing its value as a spice. In addition, the volatile oil with low polarity contains water vapor, which is difficult to separate.

[0007] 2. Solvent extraction method Extraction is done by using organic solvents such as petroleum ether (30-60°C), carbon disulfide, and carbon tetrachloride. The extraction method can be reflux extraction or cold immersion. After the organic solvent is evaporated under reduced pressure, the extract is obtained. The extract is then dissolved in hot ethanol, and the essential oil is obtained after the ethanol is recovered. The biggest disadvantage of this method is that there may be a risk of solvent residue, which requires a strict purification process; the organic solvent used may be toxic and flammable, and the operation requires strict safety measures; the use and discharge of solvents may have an impact on the environment.

[0008] 3. Supercritical CO2 extraction Using carbon dioxide, ethane, nitrous oxide and other media, through a certain temperature and pressure to make them reach a supercritical state, used to extract aromatic volatile components from medicinal materials. However, the effect of extracting essential oils and ginsenosides from ginseng by this method needs to be further improved. For example, the prior art (Zuo Xu. Research on the anti-inflammatory activity of ginseng volatile oil [D]. Jilin University) discloses the use of supercritical CO2 to extract ginseng volatile oil, but its yield is only 0.5%.

[0009] At present, the yield of ginseng volatile oil in the existing technology is less than 3%, and the content of ginseng arginol needs to be further improved.

[0010] Therefore, it is very necessary to develop a method for extracting ginseng essential oil by combining a low eutectic solvent-supercritical technology with column chromatography that can solve the above-mentioned technical problems. Summary of the invention

[0011] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for extracting ginseng essential oil by combining a low eutectic solvent-supercritical technology with column chromatography, which has high yield, high ginsenoside content, short extraction cycle and no environmental pollution.

[0012] The present invention is achieved through the following technical solutions: A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, comprising the following steps: (1) Ginseng roots are crushed and added into a supercritical extraction kettle, and supercritical extraction is performed under the combined action of liquid CO2 and an entrainer to obtain an extract; the entrainer includes anhydrous ethanol and a low eutectic solvent; (2) The extract is concentrated until there is no ethanol, diluted with water, separated by column chromatography, adsorbed on a macroporous resin for column chromatography, washed with water, eluted with 2%-5% ethanol, and eluted with 10%-20% ethanol in sequence, and the 10%-20% ethanol eluate is collected and dried.

[0013] As an embodiment of the present invention, the water content of the ginseng roots in step (1) is not higher than 9%, which can be achieved by the following operations: washing the fresh ginseng roots, and drying them by low-temperature drying at a temperature of 30°C-60°C to avoid loss of ginseng essential oil. The water content after drying is not higher than 9%.

[0014] As an embodiment of the present invention, in step (1), the ginseng roots are crushed and then passed through a 20-60 mesh sieve.

[0015] As an embodiment of the present invention, the supercritical extraction in step (1) is performed at a pressure of 15 MPa-45 MPa, a temperature of 25° C.-60° C., and a time of 1-2 h.

[0016] As an embodiment of the present invention, the volume ratio of the anhydrous ethanol to the low eutectic solvent is 9:1-15:1.

[0017] As an embodiment of the present invention, the low eutectic solvent is an anhydrous system.

[0018] As an embodiment of the present invention, the dosage ratio of ginseng root to entraining agent is 1 g / mL-6 g / mL.

[0019] As an embodiment of the present invention, the flow rate of the liquid CO2 is 100-140 L / h.

[0020] As a preferred embodiment of the present invention, step (1) is as follows: Grind the ginseng roots and add them into a supercritical extraction kettle, continuously input CO2 into the supercritical extraction kettle, slowly increase the pressure of the supercritical extraction kettle to make the extraction pressure reach 15MPa-45MPa, and the extraction temperature is 25℃-60℃; after the pressure and temperature of the extraction kettle reach the rated value, slowly open the vent valve, and after the air in the kettle is exhausted, close the vent valve; input liquid CO2 and entrainer into the extraction kettle for supercritical extraction to obtain an extract.

[0021] As an embodiment of the present invention, the deep eutectic solvent comprises at least one of the following combinations: ① Combination A comprising polyol and sugar; ② A combination B comprising a polyol and a carboxylic acid; ③ A combination C comprising at least two polyols; ④ Combination D comprising a quaternary ammonium salt and a hydrogen bond donor; ⑤ Combination E comprising carboxylic acid and sugar.

[0022] As a preferred embodiment of the present invention, the hydrogen bond donor comprises at least one of polyols, sugars, urea and carboxylic acids.

[0023] As a preferred embodiment of the present invention, the polyol includes at least one of glycerol, propylene glycol and xylitol.

[0024] As a preferred embodiment of the present invention, the sugar includes at least one of fructose, maltose, sucrose, lactose, galactose and glucose.

[0025] As a preferred embodiment of the present invention, the carboxylic acid includes at least one of malic acid, citric acid and lactic acid.

[0026] As a preferred embodiment of the present invention, the quaternary ammonium salt includes choline chloride.

[0027] As a preferred embodiment of the present invention, the molar ratio of polyol to sugar in combination A is 1-4:1-4, more preferably 1-3:1.

[0028] As a preferred embodiment of the present invention, the molar ratio of the polyol to the carboxylic acid in combination B is 1-4:1-4, more preferably 1:1.

[0029] As a preferred embodiment of the present invention, the molar ratio of the two polyols in combination C is 1-4:1-4, and more preferably 1:1.

[0030] As a preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to the hydrogen bond donor in combination D is 1-4:1-4.

[0031] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to the polyol in combination D is 1-2.5:1-4.

[0032] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to the sugar in combination D is 1-4:1.

[0033] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to urea in combination D is 1:2.

[0034] As a more preferred embodiment of the present invention, the molar ratio of the quaternary ammonium salt to the carboxylic acid in combination D is 1-2:1-4.

[0035] As a preferred embodiment of the present invention, the molar ratio of carboxylic acid to sugar in combination E is 1-4:1-4, more preferably 1-2:1.

[0036] As a preferred embodiment of the present invention, step (2) is as follows: The extract is concentrated by vacuum distillation until there is no ethanol, diluted 20 times with water, separated by column chromatography, adsorbed on a macroporous resin for column chromatography, washed with water, eluted with 2%-5% ethanol, and eluted with 10%-20% ethanol, and the 10%-20% ethanol eluate is collected and dried to obtain the product.

[0037] As an embodiment of the present invention, the macroporous resin in step (2) includes at least one of D101, AB-8 and D4020 macroporous resins.

[0038] As an embodiment of the present invention, the drying method in step (2) is evaporation under reduced pressure.

[0039] The beneficial effects of the present invention are: The present invention adopts the low eutectic solvent-supercritical technology combined with column chromatography technology for the first time, and the yield of the ginseng essential oil extracted is greater than 1.0%, wherein the content of ginseng ynol is relatively high, and the content of ginseng ynol is greater than 10%.

[0040] The invention performs drying pretreatment on ginseng roots, thereby increasing the yield of ginseng essential oil and the content of panaxynol in the ginseng essential oil within a specific water content range.

[0041] The present invention further optimizes the volume ratio of anhydrous ethanol and the deep eutectic solvent, and the composition of the deep eutectic solvent, thereby further improving the yield of ginseng essential oil and the content of panaxynol in the ginseng essential oil.

[0042] The method of the invention has high yield, few impurities, short extraction time, no environmental pollution, and can efficiently prepare ginseng essential oil rich in panaxynol. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a chromatogram of the ginseng essential oil sample obtained under the composition of the DES-1 deep eutectic solvent in Example 1. The ordinate (%) represents the relative abundance.

[0044] Figure 2 This is the chromatogram of the panaxynol reference substance in Example 1. The ordinate (%) represents the relative abundance.

[0045] Figure 3 This is the chromatogram of the ginseng essential oil sample obtained in Example 2, and the ordinate (%) represents the relative abundance.

[0046] Figure 4 This is the chromatogram of the panaxynol reference substance in Example 2, and the ordinate (%) represents the relative abundance.

[0047] Figure 5This is the chromatogram of the ginseng essential oil sample obtained in Example 3, and the ordinate (%) represents the relative abundance.

[0048] Figure 6 This is the chromatogram of the panaxynol reference substance in Example 3. The ordinate (%) represents the relative abundance. DETAILED DESCRIPTION

[0049] The present invention will be further described below in conjunction with specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are exemplary only and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

[0050] Example 1 A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, comprising the following steps: (1) Wash the fresh ginseng roots and dry them by low-temperature drying at 45°C. The moisture content after drying is 5%.

[0051] (2) The dried ginseng roots are crushed and passed through a 40-mesh sieve, and added to a supercritical extraction kettle. CO2 is continuously introduced into the supercritical extraction kettle, and the pressure of the supercritical extraction kettle is slowly increased to make the extraction pressure reach 30 MPa. The extraction temperature is 45°C and the extraction time is 1.5 h. After the pressure and temperature of the extraction kettle reach the rated value, the vent valve is slowly opened to remove the air in the kettle and then the vent valve is closed. Liquid CO2 and entrainer are introduced into the extraction kettle for supercritical extraction to obtain an extract. The ratio of the amount of dried ginseng root to the entrainer is 1 g / 0.3 mL, the entrainer is a combination of anhydrous ethanol and a low eutectic solvent, the volume ratio of the anhydrous ethanol and the low eutectic solvent is 12:1, and the composition of the low eutectic solvent is shown in Table 1; the liquid CO2 flow rate is 120 L / h.

[0052] Table 1 Composition of deep eutectic solvents

[0053] (3) The extract is concentrated by vacuum distillation until there is no ethanol, diluted 20 times with water, separated by column chromatography, adsorbed on D101 macroporous resin, washed with water, eluted with 2% ethanol, and eluted with 10% ethanol in sequence, the 10% ethanol eluate is collected, and evaporated to dryness under reduced pressure to obtain ginseng essential oil.

[0054] Example 2 A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, comprising the following steps: (1) Wash the fresh ginseng roots and dry them by low-temperature drying at 30°C. The moisture content after drying is 7%; (2) The dried ginseng roots are crushed and passed through a 60-mesh sieve, and added to a supercritical extraction kettle. CO2 is continuously introduced into the supercritical extraction kettle, and the pressure of the supercritical extraction kettle is slowly increased to make the extraction pressure reach 45 MPa. The extraction temperature is 25°C and the extraction time is 2 hours. After the pressure and temperature of the extraction kettle reach the rated value, the vent valve is slowly opened to remove the air in the kettle and then the vent valve is closed. Liquid CO2 and entrainer are introduced into the extraction kettle for supercritical extraction to obtain an extract. The dosage ratio of the dried ginseng root to the entrainer is 1:1 (g / mL), the entrainer is a combination of anhydrous ethanol and a low eutectic solvent (DES-5), the volume ratio of anhydrous ethanol to the low eutectic solvent (DES-5) is 9:1, and the flow rate of the liquid CO2 is 100 L / h; (3) The extract is concentrated by vacuum distillation until there is no ethanol, diluted 20 times with water, and then separated by column chromatography. The extract is adsorbed on AB-8 macroporous resin for column chromatography, and washed with water, eluted with 5% ethanol, and eluted with 20% ethanol in sequence. The 20% ethanol eluate is collected and evaporated to dryness under reduced pressure to obtain ginseng essential oil.

[0055] Example 3 A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, comprising the following steps: (1) Wash the fresh ginseng roots and dry them by low-temperature drying at 60°C. The moisture content after drying is 4%. (2) The dried ginseng roots are crushed and passed through a 20-mesh sieve, and added to a supercritical extraction kettle. CO2 is continuously introduced into the supercritical extraction kettle, and the pressure of the supercritical extraction kettle is slowly increased to make the extraction pressure reach 15MPa. The extraction temperature is 60°C and the extraction time is 1h. After the pressure and temperature of the extraction kettle reach the rated value, the vent valve is slowly opened to remove the air in the kettle and then the vent valve is closed. Liquid CO2 and entrainer are introduced into the extraction kettle for supercritical extraction to obtain an extract. The ratio of the dried ginseng root to the entrainer is 6:1 (g / mL), the entrainer is a combination of anhydrous ethanol and a low eutectic solvent (DES-10), the volume ratio of anhydrous ethanol to the low eutectic solvent (DES-10) is 15:1, and the flow rate of the liquid CO2 is 140 L / h; (3) The extract is concentrated by vacuum distillation until there is no ethanol, diluted 20 times with water, and then separated by column chromatography. D4020 macroporous resin is adsorbed for column chromatography, and the extract is washed with water, eluted with 3% ethanol, and eluted with 15% ethanol in sequence. The 15% ethanol eluate is collected and evaporated to dryness under reduced pressure to obtain ginseng essential oil.

[0056] Comparative Example 1 The only difference from Example 2 is that the moisture content of the ginseng roots after drying is different, which is 20%. The other conditions are the same. Step (1) is specifically as follows: (1) Wash the fresh ginseng roots and dry them by low-temperature drying at 30°C. The moisture content after drying is 20%.

[0057] Comparative Example 2 The only difference from Example 2 is that the volume ratio of anhydrous ethanol to the deep eutectic solvent is different, which is 4:1, and the other conditions are the same.

[0058] Comparative Example 3 The only difference from Example 2 is that the volume ratio of anhydrous ethanol to the deep eutectic solvent is different, which is 30:1, and the other conditions are the same.

[0059] Comparative Example 4 The only difference from Example 1 is the molar ratio of the polyol to the sugar in the low eutectic solvent, specifically the combination of glycerol and fructose, the molar ratio of the two is 6:1, and the other conditions are the same.

[0060] Comparative Example 5 The only difference from Example 1 is that the molar ratio of the polyol to the sugar in the low eutectic solvent is different, specifically, the combination of glycerol and fructose, the molar ratio of the two is 1:5, and the other conditions are the same.

[0061] Test Example 1 Determination of ginseng essential oil yield and panaxynol content (1) Materials and instruments Methanol and acetonitrile were of UPLC-MS grade; formic acid was of MS grade; leucine enkephalin and sodium formate were purchased from Waters Technologies Corporation, USA; other reagents were of analytical grade. Panaxynol was purchased from Bio CuiRan Biotechnology Co., Ltd.

[0062] Waters Xevo G2-S Q-Tof quadrupole time-of-flight mass spectrometer, ACQUITYUPLC ultra-high performance liquid chromatograph, MasslynxTM V4.1 workstation; N-A35 nitrogen generator (Shanghai Jinlang Technology Co., Ltd.); PTX-FA2105 electronic balance (Fujian Huazhi Electronic Technology Co., Ltd.).

[0063] (2) Experimental methods Preparation of test and reference solutions: Weigh 10 mg of ginseng essential oil prepared in each example or comparative example, dissolve it in 10 ml of methanol to obtain a test solution, filter it through a 0.22 μm microporous membrane, and set it aside. Weigh 1 mg of ginsenoside reference substance, dissolve it in 10 ml of methanol to obtain a reference solution, filter it through a 0.22 μm microporous membrane, and set it aside.

[0064] (3) Instrument conditions Chromatographic conditions: A Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) was used, column temperature: 30 °C; sample manager temperature: 15 °C; flow rate: 1.5 ml / min; mobile phase A: 0.1% formic acid in water; mobile phase B: acetonitrile (0.1% formic acid); gradient elution program: 0-2 min, 30% A, 10% B; 2-26 min, 30%→0% A, 70%→100% B; 26-28 min, 0% A, 100% B; 28-29 min, 0%→30% A, 100%→70% B; 29-30 min, 30%A, 70% B.

[0065] Mass spectrometry conditions: Electron Spray Ionization (ESI) positive ion (ESI + ) mode, source temperature was 120 ℃; desolvation temperature was 400 ℃; cone voltage was 40 V; cone gas flow rate was 50 L / h; desolvation gas flow rate was 788.0 L / h; capillary voltage was 2.15 kV; MSE mode was used to fully collect mass spectrometry data, the low energy channel energy was set to 6 V, and the high energy channel energy range was set to 20 V~40 V; the mass scan range was set to the m / z 150~290 Da interval; the real-time calibration solution was 100 ng / ml leucine enkephalin (ESI + mode, m / z 556.2771), the real-time correction flow rate was 15 μl / min, the sample injection volume was 5 μl, and the Masslynx V4.1 workstation was used for data processing and management.

[0066] (4) Content determination method Record the peak area and determine the content of panaxynol in the ginseng essential oil sample by external standard method. Weigh the mass of ginseng essential oil and calculate the yield of ginseng essential oil.

[0067] (5) Experimental results The experimental results of Example 1 are shown in Table 2, and the experimental results of Example 2, Example 3 and Comparative Examples 1 to 5 are shown in Table 3.

[0068] Table 2 Example 1 Ginseng essential oil yield and ginsenoside content

[0069] Table 3 Ginseng essential oil yield and ginsenoside content in Example 2, Example 3 and Comparative Examples 1-5

[0070] The chromatograms of the ginseng essential oil sample and the ginsenoside reference substance under the composition of the DES-1 deep eutectic solvent in Example 1 are shown in Figure 1 and Figure 2 .

[0071] The chromatograms of the ginseng essential oil sample and the ginsenoside reference substance obtained in Example 2 are shown in Figure 3 and Figure 4 The chromatograms of the ginseng essential oil sample and the ginsenoside reference substance obtained in Example 3 are shown in Figure 5 and Figure 6 .

[0072] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A method for extracting ginseng essential oil by deep eutectic solvent-supercritical technology combined with column chromatography, characterized in that: The steps include: (1) Ginseng roots are crushed and added into a supercritical extraction kettle, and supercritical extraction is performed under the combined action of liquid CO2 and an entrainer to obtain an extract; the entrainer includes anhydrous ethanol and a low eutectic solvent; the water content of the ginseng roots is not higher than 9%; (2) The extract is concentrated until there is no ethanol, diluted with water, and adsorbed on a macroporous resin for column chromatography. The extract is washed with water, eluted with 2%-5% ethanol, and eluted with 10%-20% ethanol in sequence. The 10%-20% ethanol eluate is collected and dried.

2. The method according to claim 1, characterized in that The deep eutectic solvent comprises at least one of the following combinations: ① Combination A comprising polyol and sugar; ② A combination B comprising a polyol and a carboxylic acid; ③ A combination C comprising at least two polyols; ④ Combination D comprising a quaternary ammonium salt and a hydrogen bond donor; ⑤ Combination E comprising carboxylic acid and sugar.

3. The method according to claim 2, characterized in that The hydrogen bond donor comprises at least one of polyols, sugars, urea and carboxylic acids.

4. The method according to claim 2 or 3, characterized in that: The polyol includes at least one of glycerol, propylene glycol and xylitol; the sugar includes at least one of fructose, maltose, sucrose, lactose, galactose and glucose; the carboxylic acid includes at least one of malic acid, citric acid and lactic acid; and the quaternary ammonium salt includes choline chloride.

5. The method according to claim 2 or 3, characterized in that: The molar ratio of polyols to sugars in combination A is 1-4:1-4; the molar ratio of polyols to carboxylic acids in combination B is 1-4:1-4; the molar ratio of the two polyols in combination C is 1-4:1-4; the molar ratio of quaternary ammonium salts to hydrogen bond donors in combination D is 1-4:1-4; and the molar ratio of carboxylic acids to sugars in combination E is 1-4:1-4.

6. The method according to claim 5, characterized in that The molar ratio of polyols to sugars in combination A is 1-3:1; the molar ratio of polyols to carboxylic acids in combination B is 1:1; the molar ratio of the two polyols in combination C is 1:1; and the molar ratio of carboxylic acids to sugars in combination E is 1-2:

1.

7. The method according to claim 1, characterized in that In step (1), the ginseng roots are crushed and passed through a 20-60 mesh sieve.

8. The method according to claim 1, characterized in that The supercritical extraction pressure in step (1) is 15MPa-45MPa, and the temperature is 25°C-60°C; the macroporous resin in step (2) includes at least one of D101, AB-8 and D4020 macroporous resins.

9. The method according to claim 1, characterized in that: The volume ratio of the anhydrous ethanol to the low eutectic solvent is 9:1-15:1; and the low eutectic solvent is an anhydrous system.

10. The method according to claim 1, characterized in that The dosage ratio of the ginseng root to the entraining agent is 1 g / mL-6 g / mL, and the flow rate of the liquid CO2 is 100-140 L / h.

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